Phenolic compounds are naturally occurring secondary metabolites from various plants. These compounds play important roles in plant such as antioxident, antibacterial and anticancer effects. The purpose of this research was to analyze the phenolic compounds contents in Kenaf leaf and stem using High Performance Liquid Chromatograpy. Kenaf is widely distributed throughout the country and third largest fiber crop. Recently, interest in this crop is growing. But, there is little research on their functional material. The obtained results showed that phenolic compounds were significantly various among the examined cultivars of Kenaf. Especially, phenolic compounds contents of leaf were higher than those of stem. The highest concentration of phenolic compounds was in the E41 leaf(24358.99㎍/g) and the lowest concentration was in the Kenaf (in korea) stem(988.21㎍/g). The results of this experiment showed that its contents were different in leaf and stem. These results suggested that Kenaf can be used for functional plant.

Gangjeong is a Korea traditional sweet cookie. Since long ago, Koreans have made Gangjeong at home whenever they have a feast such as a wedding or an ancestral rite. Gangjeong is made of rice or brown rice, honey, and oil, and has many sizies and shapes. They soak the fried pieces of Gangjeong in malt syrup, and produce different colored cookies by covering them with flour made from sesame, beans, pine nuts, pine pollen or grains of boiled rice dyed various colors. Recently, types of Gangieong has varied as the well-being trends. This experiment was conducted to examine the concentration of phenolic compounds the 24 types of Gangjeong finished goods and raw material. The total average concentration of phenolic compounds was 2340.66㎍/g and the most and the least were detected in cinnamon powder(21143.10㎍/g) and rice(49.40㎍/g), respectively. Cinnamon, green-tea powder and others had the highest content of phenolic compounds in the raw materisls. And the finished gangjeong, rice plus curry products exhibited the highest content of total phenolic compounds(1221.60㎍/g). Among the individual phenolic compounds, benzoic acid showed the highest (379.24㎍/g) concentration and sallicylic acid was the lowest (0.34㎍/g) consitutent.

Cereal grains are sort of crops like barley, sorghum, millet, oats, rye and many others. Research of other scientists were shown that whole grain consumption helps lower the risk of cardiovascular disease, ischemic stroke and gastrointestinal cancers. They are rich source of carbohydrates, protein, fats and oils, and have many health-promoting components such as vitamins, minerals, and phytochemicals, which include phenolic compounds. This research was conducted quantitative analysis about phenolic compounds in 15 lines of cereals by high performance liquid chromatography(HPLC). Samples are 5 of Sorghum bicolor Moench, 5 of Setaria italica, 5 of Panicum miliaceum L. they are harvested from Kyungbook-bongwha in 2009. The phenolic compounds contents of Moktak-sorghum (1187.298㎍/g), Hwanggeumchal-sorghum(897.7417㎍/g) and Hwanggeum-sorghum(762.8306㎍/g) are the highst, those have dark color between Sorghum bicolor Moench. Analysis Among the contents of Sorghum bicolor Moench (+)Catechin(avg. 139.95㎍/g) and Benzoic acid(avg.116.695㎍/g) were higher contents than others. Myricetin(avg.46.15㎍/g) and Quercetin(avg. 47.4368㎍/g) were many contents in Steria italica. Chlorogenic acid(avg. 46.258㎍/g) and Quercetin(avg. 43.0496㎍/g) were many contents in Panicum miliaceum L. The result of this experiment is considered to be the basic data of the functional food manufacturing by utilizing domestic cereal grains.

In this study, we examined the variation of 30 phenolic compounds in the mungbean (Vigna radiata (L.) Wilczek) germplasms. Recent studies showed that the potential health benefits from the antioxidnant activity of the phenolic compounds. The average concentration of phenolic compounds among the 70 mungbean germplasms was 2424.9 μg g-1. Among the mungbean germplasms, 25 kinds of mungbean germplasms were higher than others. Specially, 69 (IT 182280) (3836.63 ㎍ g-1) and 63 (IT 180525) (3491.51 ㎍ g-1) revealed highest levels of the total phenolic compounds. On the other hand, 27 (IT 154078) (1624 ㎍ g-1) was revealed lowest concentration of the total phenolic compounds. Among the individual phenolic compounds, rutin (avg. 1776.09 ㎍ g-1) showed highest concentrations, pyrogallol (144.31 ㎍ g-1), gentisic acid (119.19 ㎍ g-1) and chlorogenic acid (78.24 ㎍ g-1) were relatively higher than other phenolic compounds. The germplasm of 69 (IT 182280) had the highest rutin (3156.87 ㎍ g-1) concentraion. While, the biochanin A (avg. 2.02 ㎍ g-1) and formonetin (avg. 2.61 ㎍ g-1) had the lowest levels among the mungbean germplasms. This study indicateded that determination of the functional substances in mungbean germplasms and it is more valuable for the future crop cultivation and food industries.

family in the Brassica genome sequences by computational approach. The MITE family showed a total of 264bp length including 36bp terminal inverted repeats and remained 2bp (TA) targets it eduplication by its insertion. By searching the genome database of Brassica species, 516, 227, and 15 members were identified from 470Mbp of Brassica oleraceae, 154Mbp of B.rapa and 15Mbp of B.napus, respectively, indicating that there are approximately 692, 760, 1235 copies in B.oleracea, B.rapa and B.napus genomes,respectively. A total of 225 relatively intact MITE members, 146,68, and 11 members, which showed >80% sequence similarity and sequence coverage were identified and retrieved for MITE analysis from B.oleracea, B.rapa and B.napus genomes, respectively. Out of 225 MITE family members 159 having full structure of MITE and 66 having the truncated end either in right TIR or left TIR. Insertion polymorphism due to insertion or non-insertion of MITEs showed high level of polymorphism among accessions intra and inter species of Brassica. The new MITE would provide abetter tool for study molecular breeding in Brassica species and also helpful to understand their contribution in evolution and diversification of the highly duplicated Brassica genome.

Perilla is a genus as a member of the mint family Lamiaceae which is known to contain lots of volatile metabolite. Perilla has been called as ‘deulkae’ indicating ‘wild sesame’ that means it has been maintained in Korea with long history. It has been very friendly used as edible oil and as fresh leaf vegetable. Perilla oil is valued for its medicinal benefit because it contains best amounts of unsaturated fatty acids, especially for the alpha-linolenic acid, known to omega-3 fatty acid, among all of the plant oils. It also include many beneficial phytochemicals. However, little study is conducted on their genetics. Here, we announce construction of well normalized and full length enriched-perilla cDNA library from a whole plant of one cultivar ‘Youngho-deulkae’ and their sequence characterization to provide useful resources for genetics, breeding and metabolite engineering. By sequencing of 5,760 cDNA clones, we 5,438 high quality EST sequences. Sequence trimming and assembly resulted 3,995 unigenes which consists 1,004 contigs and 2,991 singletones. Unigenes that showed little homology at the DNA sequence level with known genes in other plants even though they showed similarity at the protein domain level based on BLASTN, BLASTX, and TBLASTX. This study may provide good resources for initiation of further genomics, comparative genomics, functional genomics such as metabolic engineering and molecular breeding.

Experiments were conducted to investigated the change of primary dormancy and viability of Chinese milk vetch(CMV) seed buried in soil both as seed and pod with seeds under CMV-rice cropping system during the period of 2007～2009. The freshly harvested CMV seed alone and pods with seeds were buried in rice field at 0, 5 and 10cm depths and determined change of seed dormancy and viability at one to three month intervals for 10 months. The CMV seed had high dormancy of 95%, showing only 4～5% germination at the beginning in June but the seed dormancy was gradually broken at rice harvest time in autumn, showing 25 to 35% for seed and 55 to 61% for pod with seeds. The viability loss was faster in the seed than in the pod with seeds regardless of depths of placement in the soil base on decayed seeds. Also the seed placed on the soil surface lost viability faster than the 5～10 burial depths. However, appreciable number of CMV seeds still remained at even 4 months after burial in soil. These results indicate that seed dormancy was enable CMV plant to regenerate naturally from the remained soil seed bank at rice harvest time in autumn.

To facilitate the introgression of F. esculentum into the traits of F. homotropicum, several accessions of the hybrids between these two species were pollinated with F. esculentum as the recurrent parent. In vitro embryo rescue was performed to increase the recovery of backcross progenies. The F2 generation was more amenable than F1 hybrids to produce backcross progenies. The F1 hybrids were backcrossed twice with common buckwheat (pin-type F. esculentum, recurrent backcrossing). Also, alternate backcrosses with common buckwheat and F. homotropicum (congruity backcrossing) were carried out. Pollen tube growth of BCF1 x F. esculentum (thrum) and F. homotropicum x BCF1 was disturbed penetration exceeded for all initial interspecific hybrids, and its requirement was proportionally lower when the common buckwheat used as the recurrent parent and as the last parent of congruity hybrids. Effects of both common buckwheat and F. homotropicum on seed success rate for hybridization were observed. Growth of hybrid embryos before rescue, regeneration of mature hybrids all increased recurrent and congruity backcrosses, inter-crosses between F1 plants and selected fertile plants of the second congruity backcrosses.

Field resistance is defined as the resistance that allows effective control of a parasite under natural field condition and is durable when exposed to new races of that parasite. To identify the genes for field resistance against rice blast, quantitative trait loci (QTLs) conferring field resistance in japonica rice cultivars were detected and mapped using SSR markers. QTL analysis was carried out in 190 RILs population from the cross between Suweon365 (moderately resistance) and Chucheong (highly susceptible). Fourteen QTLs for nine blast races inoculated were detected on chromosomes 1, 2, 4, 5, 6, 7, 11 and 12. They explained from 6.4 to 39.7% of total phenotypic variation. Eight QTLs for blast nursery screening in 4 regions for three years were detected on chromosomes 1, 2, 4, 5, 11 and 12. The phenotypic variation was explained by each QTL ranged from 5.9 to 38.0%. Three BC2F5 backcrossed progeny lines were developed to transfer the QTLs into the susceptible cultivar Chucheong as a recurrent parent. A NIL4 containing two QTLs Qbl6.2 and Qbl7 for blast races showed the reaction 6 to 7 in blast nursery in 2007 and 2008, respectively. Two lines NIL143 and NIL93 containing Qbl11.2 and Qbl12.1 for QTLs related with field resistance, respectively, were 3 to 4 reactions in blast nursery.

Switchgrass is a wild-perennial plant in North America, and rich in cellulose, making it attractive as a source for cellulosic ethanol. Especially, ethanol which is made from switchgrass produce more 540% energy better than the others. The objectives of this study were to examine the concentrations of phenolic compounds, monosaccharides and disaccharides which detected in some different part (upper, center, lower) of 34 species of switchgrasses. The total average of phenolic compound concentration was 4017.48 ㎍/g. Whereas it's concentration in the upper part was shown the highest (6669.09㎍/g) and the lower part was the lowest (1916.30㎍/g). The No.23 has the highest concentration of phenolic compounds but No.20 have the lowest concentration. In saccharides analysis, only arabinose and glucose are detected. The total average of monosaccharides and disaccharides concentration was 206284.75 ㎍/g. Whereas its concentration in the upper part was shown the highest (269738.18㎍/g) and the lower part was the lowest (167567.53㎍/g). The No.30 has the highest concentration of saccharides but No.2 have the lowest concentration. The obtained results shows that switchgrass are good sources of bio-ethanol and the upper part is the best among them.

Medicinal soybean, which has been proven to be effective in preventing cancer, heart disease, osteoporosis, and renal failure, is drawing people’s attention among other beans in recent days. This experiment was to examine the concentration of phenolic compounds and isoflavone in 53 kinds of soybeans cultivated and collected in Yeo-Ju, Gyeonggi-do region. The total average content of phenol is 29424.34㎍/g and the most and least phenol were detected in No. 35(814.98㎍/g) and No. 28(353.66㎍/g) respectively. Myricetin was detected the most resveratrol whereas detected the least amount which was less than 0.2% of the total amount among all medicinal soybean samples. Total average isoflavone content was 35910.56㎍/g and the most and least isoflavone was detected in No. 15 (996.66 ㎍/g ) and No.9 (476.18㎍/g )respectively . Malonylgenistin was detecteded the most genistein whereas detected the least amount which was less than 0.02 % of the total amount in all medicinal soybean samples. The result of this experiment is considered to be the basic data of the functional food manufacturing by utilizing domestic medicinal soybean.

Soybean(Glycine max(L.) Merill) is a good source of vegetable oil and high-quality protein. Soy foods have became popular food worldly because of its ingredients. Recently, as the well-being trends, the demand for soybean has increased in the West as well as in Asian countries. It contains valuable source of dietary isoflavones and phenolic compounds which are important secondary metabolites. The 33 germplasm cultivated in Yeoju, Gyeonggi-do region. The obtained results showed that isoflavones and phenolic compounds were significantly various. The highest compounds is malonyl genistin (230.89㎍𝑔-1), the lowest is genistin(0.70㎍𝑔-1) and the highest germplasm is Dunggeunseolitaekong (2222.60㎍𝑔-1) in isoflavone. In phenolic compounds, the highest compounds is myricetin (155.59㎍𝑔-1) and the lowest is vanillin (1.89㎍𝑔-1). Total containment of phenolic compounds is the highest in Dunggeunseolitaekong (803.76㎍𝑔-1) germplasm.

The leaves of Catharanthus roseus (L.) G. Don. is the source of pharmaceutically valuable terpenoid indole alkaloids (TIAs) such as anticancer drugs vinblastine and vincristine. In particular, vindoline and catharanthine are harvested from leaves and used for the semisynthetic production of vinblastine and vincristine. Because of this, catharanthine and vindoline can be used to screen for high-yielding TIA cultivars. In this study we determined the TIAs concentrations of the leaves of 77 different cultivars of C. roseus. Serpentine contents among the 77 germplasms showed highest concentration in Vinca Pacifica Apricot (519.0 μg/g DW) while vindoline (2082.3 μg/g DW) and catharnathine (2903.4 μg/g DW) were the highest in Vinca Pacifica Peach. Additionally, Vinca Pacifica Peach (5041.0 μg/g DW) had the highest total TIAs concentrations. The 77 germplasms were classified into seven types according to their characteristic: Vinca Sunshower, Vinca Sunstorm, Vinca Cooler, Vinca Pacifica, Vinca Mediterranean, Vinca Titan, Vinca Stardust. Among the seven types, Vinca Sunstorm (3120.4 μg/g DW) had the highest average total TIAs concentrations in C. roseus leaves. This study indicates that cultivars of Vinca Sunstorm type have the potential to be industrial important cultivars for the production of vinblastine and vincristine.