The purpose of this study was to investigate the effect of kind and combination of sugars on the viability and acrosome damage of post-thaw spermatozoa in canine. The extender used was Tris-citric acid extender (Tris-buffer) supplemented with 20% Egg-yolk, 8% glycerol, 1% Equex STM paste, and 70 mM sugars such as monosaccharide (fructose and xylose) and disaccharide(trehalose). To evaluate of sugar combination, the sugars supplemented in Tris-buffer were combined such as single (fructose, xylose, trehalose), two combinations (Fruc+Tre, Fruc+xyl, Tre+xyl) and three combinations (Fruc+Tre+Xyl). The concentration of sperm collected were adjusted of 50106/ per straw for freezing. The frozen spermatozoa were thawed at 37 for 1 min and then analysis for CASA program in Livestock Improvement Main Center, NACF. The motility of post-thaw spermatozoa in Fruc+Tre was higher than those in fructose, trehalose, xylose, Fru+xyl, Tre+xyl and Fru+Tre+Xyl (79％ vs. 63, 66, 70, 71, 74 and 75%). The progressive motility after CASA analysis in Fuc+Tre group was also higher than those in fructose, trehalose, xylose, Fru+xyl, Tre+xyl and Fru+Tre+Xyl (67% vs. 53, 57, 60, 61, 62 and 64%). The acrosome damage of post-thaw spermatozoa stained was not significantly different among treatment groups such as fructose, trehalose, xylose, Fru+Tre, Fru+xyl, Tre+xyl and Freu+tre+xyl (17.7, 18.3, 28.0, 17.0, 19.7, 20.0 and 19.0%). The results indicated that the motility and progressive motility of post-thaw spermatozoa in Fru+Tre group was better, and acrosome normality was not different among all groups. The use of Tris-buffer supplemented with Fru+Tre as sugar for frezing of canine spermatosoa could be better and apply to semen banking and artificial insemination.

본 연구는 체내, 체외 소 배반포기 배의 GMP vitrification 후 활력도의 비교와 한우 수정란을 몽골 소에 수정란이식 후 산자생산 가능성을 조사하고자 실시하였다. 한우 수정란은 체외수정란 또는 과배란처리에 의한 체내수정란을 생산하여 GMP vitrification 방법으로 동결 후 몽고로 수송하였다. 수란우는 CIDR과 처리에 의하여 동기화를 유도하였다 체내수정란생산을 위하여 7두를 과배란처리하였다. 총 64개의 배반포기를 회수하였다. (pe

We have investigated the properties of the high-latitude cloud MBM 7 using the 3 mm transitions of CO, CS, HCN, HCO+,C3H2,N2H+, and SiO. The molecular component of MBM 7 shows a very clumpy structure with a size of ≤0.5 pc, elongated along the northwest-southeast direction, perpendicularly to an extended HI component, which could be resulted from shock formation. We have derived physical properties for two molecular cores in the central region. Their sizes are 0.1-0.3 pc and masses 1-2 M⊙ having an average volume density ~2×10 3 cm-3 at the peak of molecular emission. We have tested the stability of the cores using the full version of the virial theorem and found that the cores are stabilized with ambient medium, and they are expected not to be dissipated easily without external perturbations. Therefore MBM 7 does not seem to be a site for new star formation. The molecular abundances in the densest core appear to be much less (by about one order of magnitude) than the 'general' dark cloud values. If the depletions of heavy elements are not significant in the HLCs compared with those in typical dark clouds, our results may suggest different chemical evolutionary stages or different chemical environments of the HLCs compared with dense dark clouds in the Galactic plane.

The 3mm transitions to CO, 13CO, CS, HCO+, and HCN have been observed toward the compact HII regions in W58 using the 14m Daeduk Radio Telescope (DRT). Some of the observed lines show high-velocity wings resulted from outflowing materials of the compact HII regions. We derive the beam averaged column densities of the observed species and compare their relative abundances. The HCO+ abundance appears to be smaller by about an order of magnitude than those of 'typical' quiet molecular clouds. CS may be a good reference molecule in comparing relative abundances in different physical conditions.

벼멸구 생태형 1, 2, 3의 형태의 차이를 구별하고자 장시형과 단시형 암수를 대상으로 형태적 변화를 관찰하였다. 암컷은 생식 부위인 Abdominal lateral lobes의 좌우 부위를 비교 조사하였다. 수컷은 앞다리, 가운데 다리, 뒷다리 부절의 Unguitractor plate를 조사하였다. 생태형 2의 단시형 암컷은 Abdominal lateral lobes의 기부가 잘라진 것으로 생태형 1과 3보다 많았다. Unguitractor plate의 경우 생태형 2는 장단시형 암 수 모두 부절 안쪽으로 함입되어 있었다.

우리나라에 발생하고 있는 벼멸구 생태형의 형태적 차이를 구명하고자 생태형 1, 2, 3의 단시형 암컷과 숫컷의 다리부분의 형태를 관찰하였다. 앞다리, 가운데 다리 그리고 뒷다리의 제3부절의 형태를 51개 부위에서 조사한 다음 통계학적 분석을 위하여 정준 판별 분석법을 도입하였다. 각 생태형간의 Mahalanobis distance는 숫컷의 경우 생태형 2와 3 사이에서 가장 짧았고, 암컷은 생태형 1과 2 사이에서 가장 길었다. Scatter plot diagram상에서 각 생태 형간 분리현상이 뚜렷하여 중심점이 각각 다르게 나타났고 각 생태형에 속하는 개체는 중심점 부근에 고르게 분포하였다. 각 생태형간의 Group membership 조사에서 암수 모두 각 생태형은 각각 동일한 생텨형을 분류되었다.

벼멸구 수컷 정소에서 염색체의 변이정도를 생태형 별로 관찰하였다. 세포 분열 지수는 생태형 3에서 가장 높았고 그 다음으로 생태형 1, 생태형 2이었다. Agmatoploidy, aneuploidy, 성염색체의 loose pairings과 같은 염색체의 구조적 변화는 생태형 1, 생태형 2, 생태형 3순으로 높았다. 감수분열 제1분열기 중기는 성염색체가 상염색체로부터 현격하게 분리되어 있는 세포의 수는 생태형 2에서 가장 높았다.

To find an alternative for synthetic pesticides, methanol extracts from 69 plant samples were tested for their insecticidal activity against two spotted mite (Tetranchus urticae Koch). Seven plant extracts including Angelica japonica showed over 80% insecticidal activity at 5000 mg/L. Extract of Prunus armeniaca seed showed high insecticidal activity at 3000 mg/L. As a naturally occurring pesticide, P. armeniaca could be useful as a new botanic insecticide.

Green manure crops play an inportant role in organic farming. Field experiment was conducted at paddy soi1 (fine loamy, mixed, nonacid, mesic family of Aeric Fluvaquentíc Endoquepts) ín 2008/2009 to 2009/2010 at the Natíonallnstítute of Crop Science (NICS), RDA, Suwon, Gyeonggí provínce, Korea. This expe꺼ment was carried out to evaluate the biomass of haíry vetch (Vicia víllosa) and growth of rice (Oryza satíva) by cjífferent seedíng rates. Seeding rates of hairy vetch consisted of 30, 60, and 90 kg ha-' by broadcasting before rice harvesting. The biomass af)d nitrogen production of hairy vetch were not significantly different between 60 kg ha-’ and 90 kg ha-’ of seeding rates. Also, rice yield was not siginicantly differnt between seeding rate 60 kg ha-’ of hairy vetch and conventional pratice for two years. Therefore, we suggested that seeding rate of hairy vetch should be reduced by continuous cropping and incoportion of hairy vetch under rice-based cropping system.

Controllable transgenic expression systems in transgenic animal model are valuable to the development of therapeutic approaches in human medical fields. The aim of this study was to 1) produce a transgenic cloned dog using inducible tetracycline vector system, and 2) investigate whether the transgenic cloned dog could be induced the transgene expression using doxycycline (Doxy). Canine fetal fibroblasts were infected with retroviral vectors designed to express the enhanced green fluorescent protein (eGFP) gene under the control of tetracycline-inducible promoter. For somatic cell nuclear transfer (SCNT), nucleus of an in vivo matured oocyte was removed and an eGFP expressed cell cultured with 1 ㎍/㎖ of Doxy was injected. After electrical fusion and chemical activation, the reconstructed embryos were transferred to a recipient and pregnancy diagnosis was performed by ultrasonography. Experiment I evaluated the mean fluorescence intensity (MFI) of infected cells while the cells were cultured in the presence of 1 ㎍/㎖ of Doxy for 5 days, and then in the absence of Doxy for 7 days using fluorescence-activated cell sorter. Experiment II was designed to produce an eGFP controllable transgenic cloned dog via SCNT. For verification of transgenic dog, experiment III was performed Southern Blot analysis and observation in vivo regulation of eGFP expression in the cloned dog treated with 100 ㎎/㎏ of Doxy every 2 days for 2 weeks under ultraviolet light. In experiment IV, western blot was used to detect eGFP increase and decrease in skin tissues of transgenic dog under the presence or absence of Doxy. In the results of experiment I, the MFI for infected cells was rapidly increased to approximately 42.3 times after 3 day-treatment compared to pre-treatment and quickly decreased 3 days after ceasing the treatment. In experiment II, a total of 203 embryos were transferred to nine recipients and three pregnant delivered three pups (Tet-on eGFP 0, Tet-on eGFP 1, and Tet-on eGFP 2) by C-sec and Tet-on eGFP 2 among them is still alive. All cloned pups were genetically identical to the donor cell. Tet-on eGFP 2 showed an apparent in vivo eGFP expression on her body after Doxy administration in experiment III. The result of Sothern blotting showed that the transgene insertion was detected from the three cloned dogs and all organs of Tet-on eGFP 1. Experiment IV indicated that a robust eGFP expression in skin tissue of Tet-on eGFP 2 rapidly increased after Doxy treatment and gradually decreased to basal level on 9 weeks after ceasing the treatment. In conclusion, we report here for the first time an inducible transgenic system in canine species and it can stably induce the transgene expression at intended time. This study has demonstrated the capacity to generate transgenic model dog which could regulate the transgene and it would contribute to human medical research fields.

The cloning of canids was succeeded in 2005, several years after the birth of Dolly the sheep and also after the cloning of numerous other laboratory and farm animal species. The delay of successful somatic cell nuclear transfer (SCNT)was due to the unique reproductive characteristics of the female dogin comparison to other domestic mammals, such as ovulation of immature canine oocyte and a requirement of 25 days for the completion of meiosis within the oviduct (Holst & Phemister, 1971). When the technology for the recovery of in vivo matured oocyte was established, the application of cloning also became possible and cloned dog offspring were obtained. This report summarizes the progress of technical procedures that are required for cloning canids and the application of this technique. The first cloned dog, Snuppy, was achieved using an in vivo-matured oocyte which was enucleated and transferred with an adult skin cell of male Afghan hound. After establishment of a criterion of well-matured oocyte for the improvement of SCNT efficiency, we obtained three cloned female Afghan hound and a toy poodle cloned from 14 year-old aged Poodle using SCNT through this factor. To date, cloned dogs appeared to be normal and those that have reached puberty have been confirmed to be fertile. Through application of canine SCNT technique, first, we demonstrated that SNCT is useful for conserving the breed of endangered animal from extinction through cloning of endangered gray wolves using inter-species SCNT and keeping the pure pedigree through the cloning of Sapsaree, a Korean natural monument. Secondly, we showed possibility of human disease model cloned dog and transgenic cloned dog production through cloning of red fluorescent protein expressing dog. Finally, SCNT can be used for the propagation of valuable genotypes for making elite seed stock and pet dog. In summary, dog cloning is a reproducible technique that offers the opportunity to preserve valuable genetics and a potential step towards the production of gene targeted transgenic cloned dogs for the study of human diseases.