본 연구는 능경봉(평창군, 강릉시)지역에 대하여 2008년 8월 과 2013년 4월부터 동년 10월까지 총 7회에 걸쳐 관속식물 분포 를 조사하였다. 전체 조사된 분류군은 75과 261속 383종 4아종 48변종 5품종으로 총 440분류군이 자생하는 것으로 확인되었 다. 주요 식물로 한국특산식물이 15분류군, 산림청지정 희귀식 물 중 약관심종이 11분류군, 환경부 지정 식물구계학적 특정식 물 중 4등급이 11분류군, 3등급이 22분류군으로 확인되었다. 귀 화식물은 총 29분류군으로서 귀화율은 6.6%, 도시화지수 9.0% 로 나타났다. 그밖에 대한약전 및 대한약전외한약(생약)규격집 에 수재된 약용식물은 60분류군으로 조사되었다.

Human embryonic stem cells (hESCs) have the potential for use in regenerative medicine and in the field of basic research. Therefore, effective cryopreservation and storage of hESCs are important for preservation of newly established cell line for various purposes. Despite poor survival and slow recovery after thawing, the conventional slow freezing method is most commonly used for cryopreservation of hESCs due to its simplicity and ease of use for freezing a large number of hESCs appropriate to clinical applications. Here we controlled the clump size (Group Ⅰ; 400～450 ㎛, Group Ⅱ; 800～900 ㎛, and Group Ⅲ; 1500～1700 ㎛) of hESCs at 5 days after plating using a glass pipette during cryopreservation in order to obtain a larger amount of hESCs after thawing. Attachment rates differed significantly (P<0.05) in each of the three groups and the average of attachment rate of GroupⅡ was highest in SNUhES4 and H1. In particular, the attachment rate of Group Ⅱ in SNUhES3 showed a significant improvement with ROCK inhibitor Y-27632. These results indicate that clump size and cell-cell adhesions of GroupⅡ are appropriate for cryopreservation compared to the Group Ⅰ and Group Ⅲ. This method increased cell viability and reduced the recovery time leading to various experiments, and therefore has an advantage for use with hESCs like newly established in particular. We demonstrated that use of this effective cryopreservation method with control of the clump size of hESCs can effectively improve the attachment rate and survival of post-thaw hESCs with and without Y-27632.

"Sunny Gold", an Asiatic lily variety was released by the National Horticultural Research Institute (NHRI), in 2006. A cross was made between a yellow colored Asiatic lily variety "Mona" and a light yellow colored Asiatic lily variety "A93-15(Sunray x Foxtrot)" in 1996. Selection was made in the natural cultivation condition from the progenies derived from the cross between "Mona" and "A93-15" in 1998. The flower characteristics and performance of "Sunny Gold (line name was Wonkyo C1-88)" were investigated from 2000 to 2005. Although its flowering time is middle of the June, year-round cut flower production was possible by storing the bulbs at -1 or -2℃. It has unspotted, upward facing, and yellow petals with no fragrance. The plant height is 87.3 cm. The width of flower is 14.2 cm. The weight and circumstance of bulb are 15.2 g and 10.9 cm, respectively. For forcing culture, it is necessary to add calcium to the fertilizer or remove side scales to prevent leaf scorch. Botrytis disease should be properly controlled especially in wet season.

"Pinky Smile", an Asiatic lily variety was released by the National Horticultural Research Institute (NHRI), in 2006. A cross was made between a light pink colored Asiatic lily variety "Marlene" and a white colored Asiatic lily variety "Pinguin" in 1998. Selection was made in the natural cultivation condition from the progenies derived from the cross between "Marlene" and "Pinguin" in 2000. The flower characteristics and performance of "Pinky Smile (line name was Wongyo C1-82)" were investigated from 2001 to 2005. Although its flowering time is early June, year-round cut flower production was possible by storing the bulbs at -1 or -2℃. It has unspotted, upward facing, and ivory-pink (RP62A+W155B) petals with no fragrance. The plant height is 107 cm. The width of flower is 13.9 cm. The weight and circumstance of bulb are 39.3 g and 15.4 cm, respectively. For forcing culture, it is necessary to add calcium to the fertilizer or remove side scales to prevent leaf scorch. Botrytis disease should be properly controlled especially in wet season.

A new gerbera variety 'Claret' was released by the National Horticultural Research Institute (NHRI) in 2006. A cross was made between 'Rako' with pink and semi-double and 'Sessile' with orange and single in 2000. After investigation of the characteristics for 6 years (from 2000 to 2006), it was selected specially for the use of cut-flower. 'Claret' is vigorous cultivar with purple and semi-double. It produces a large yield with continuous supply of flowers and has adequate peduncle height and brilliant color. We believe that 'Claret' has a great potential for expanding cultivated area of domestic mini-gerbera cultivar and increasing farmers-income.

A new gerbera variety 'Peach Honey' was released by the National Horticultural Research Institute (NHRI) in 2006. A cross was made between 'Ansofie' with white and semi-double and 'Lilabella' with pink and semi-double in 2000. After investigation of the characteristics for 6 years (from 2000 to 2006), it was selected specially for the use of cut-flower. 'Peach Honey' is vigorous cultivar with salmon and semi-double. It produces a large yield with continuous supply of flowers and has adequate peduncle height and brilliant color. We believe that 'Peach Honey' has a great potential for expanding cultivated area of domestic gerbera cultivar and increasing farmers-income.

Wild rices are an important source of useful genes for resistance to diseases, insect pests, tolerance to abiotic stresses and yield increase. Interspecific hybrids were successfully produced between ten accessions of O. minuta (2n=48, BBCC) and O. sativa