Raman spectroscopy has been used to investigate the structure of coal tar pitch heat-treated up to 3000℃ by using 514.5 run Ar ion laser line. Four critical temperature ranges were found on pyrolyzing coal tar pitch, which correspond to four distinct processes from disordered carbons to the well-ordered graphite structure. The range of heat treat temperature (HTT) below 1000℃ corresponds to gas evolution during the pyrolysis of coal tar pitch. Above the HTT are correlated to rearrangements of enlarged molecules, growth of the molecules along the direction of plane, finally stacking in the normal direction of the plane, in the respective HTT ranges of 1000-2000, above 2000 and 2500-3000℃.

변색미(變色米) 발생(發生)에 관여(關與)하는 병원균(病原菌)과 해충을 분류동정(分類同定)한 결과(結果) 해충으로는 허리노린재과(科)에 속하는 Leptocorisa oratorius가 우점종(優占種)이었고 노린재과(科)인 Menida varipennis. Stollia ventralis 및 Nezara viridula 등이 관여(關與)하였으며 병원균(病原菌)으로는 Drechslera oryzae. Curvularia lunata, Trichoniella padwickii, Sarocladium oryzae, Alternaria tenuis 및 Fusarium solani 등이 관여(關與)하였다. 병원균(病原菌)과 해충의 복합발생시(複合發生時)에 변색미발생(變色米發生)이 더 심하였고 병원균(病原菌)만의 발생시(發生時)는 변색미(變色米) 발생(發生)에 주로 영향(影響)을 미쳤으며 노린재류만의 발생시(發生時)는 수량감수(收量減收)에 더 큰 영향(影響)을 주었다. 그리고 노린재류에 의한 벼 유숙기간(乳熟期間)의 흡즙(吸汁)은 병원균침입(病原菌侵入)을 조장(助長)하여 벼의 질적(質的) 변화(變化)와 양적(量的) 감소(滅少)에 크게 영향(影響)하였다.

주요 벼 해충의 경제적 피해 수준을 결정하고 약제살포 회수의 감소 가능성을 모색하기 위하여, 수원 등 4개 지역의 농가 포장 조건하에서 본 연구를 수행하였다. 무살포구 관행방제구 및 2.3의 관행 방제를 수정한 처리구를 두었으며, 해충 밀도 및 병의 발생에 대해서도 상세한 기록을 행하였다. I) 이화명충의 제1세대가 활발한 중부지방의 수원과 이천의 경우 수회 약제 살포구와 무살포구 사이의 수량의 차에 유의성이 없었으며, 평균 수량은 높아서 5.2-7.6t/ha였다. ii) 일본형 품종이나 다수성 품종인 통일 어느 품종에서도 수량의 감소를 인정할 수 없었던 고로 피해경율이 까지 상승한 제1세대 유충의 가해행위는 경제적 피해 수준 이하였다. iii) 유충에 의한 피해는 피해경이 대체되는 보상생장에 의해서 호전된다는 증거가 얻어졌다. 이화명충의 제2세대 개체군은 언제나 낮았다. iv) 수원과 이천에서 얻어진 결과를 볼 때 현재의 관행 약제 살포 회수 6-7회는 합당하지 않다. v) 남부지방인 이리의 경우 애멸구에 의한 줄무늬잎 마름병이 초기에 대발생하여 이로 인해 실질적인 수량의 감소가 일어났다. 이앙 직후에 애멸구의 평균 밀도는 주당 1-2마리였다. 이화명충도 이러한 수량의 감소에 영향을 주었을 가능성이 있다. vi) 이리에서 애멸구에 대해 수회의 약제 살포를 행하였으나 감수성 품종에서 급속하게 번져가는 줄무늬 잎마름병을 막을 수 없었으며, 그 주변 포장의 저항성 품종인 통일은 비교적 영향을 받지 않았다. vii) 끝동매미충, 벼멸구, 횐등멸구 및 벼잎굴파리는 중요성이 적은 해충이다.실효를 거둘 수 없는 반면 신문지 봉지는 피해율을 나타냈으나 숙기가 약간 지연되는 외에는 하등의 지장이 없었다. 5) 백색 전구는 비래 나방수가 10.8마리로 청색 0.95마리, 황색 전구 0.22마리에 비하여 많이 비래하였으나 방제효과 면에서는 기대하기가 어려운 것으로 본다의 밀도를 조사였다. 1. Carbofuran, Omethoate 액상근부처리에 비하여 캡슐 근부처리는 이화명충, 애멸구방제효과가 월등히 좋았고 Omethoate보다는 Carbofuran의 방제효과가 좋았다. 2. Carbofuran 1회 액상근부처리는 Carbofuran 2회 수면시용과 대등한 수도해충의 방제효과가 있었다. 3. A포장에서 보다 B포장에서 이화명충 방제효과가 좋았는데 그것은 B포장에서의 약제처리시기가 이화명충 방제적기에 해당하기 때문인 것 같다. 4. Carbofuran 1회 근부처리는 수도 전 생육기간에 걸친 수도해충방제가 가능한 것으로 보였다.이었다. 따라서 양자간의 상관관계를 밝히는데 도움이 되지는 못하였다. 그러나 본 연구기간 중 필자의 주장을 뒷받침 해 주는 중요한 새로운 정보를 입수할 수 있었다. 즉 1946년에 흰둥멸구의 대발생으로 충남, 전남지방에 피해가 심했으며 그중 피해액이 밝혀진 곳은 라주군에서 16만석, 서산군에서 19만석이었다. (당시 농사시험장 곤충담당관 이봉우씨 담, 현재선 교수 전) 1910년 이후의 우리나라에서의 벼멸구 생기록을 보면 항상 흰등멸구와 함께 발생하고 있으며 최근의 예찰등성적을 보아도 함께 나타나 있고, 해에 따라 1975년도와 같이 벼멸구가 대발생하는 수도 있

Controllable transgenic expression systems in transgenic animal model are valuable to the development of therapeutic approaches in human medical fields. The aim of this study was to 1) produce a transgenic cloned dog using inducible tetracycline vector system, and 2) investigate whether the transgenic cloned dog could be induced the transgene expression using doxycycline (Doxy). Canine fetal fibroblasts were infected with retroviral vectors designed to express the enhanced green fluorescent protein (eGFP) gene under the control of tetracycline-inducible promoter. For somatic cell nuclear transfer (SCNT), nucleus of an in vivo matured oocyte was removed and an eGFP expressed cell cultured with 1 ㎍/㎖ of Doxy was injected. After electrical fusion and chemical activation, the reconstructed embryos were transferred to a recipient and pregnancy diagnosis was performed by ultrasonography. Experiment I evaluated the mean fluorescence intensity (MFI) of infected cells while the cells were cultured in the presence of 1 ㎍/㎖ of Doxy for 5 days, and then in the absence of Doxy for 7 days using fluorescence-activated cell sorter. Experiment II was designed to produce an eGFP controllable transgenic cloned dog via SCNT. For verification of transgenic dog, experiment III was performed Southern Blot analysis and observation in vivo regulation of eGFP expression in the cloned dog treated with 100 ㎎/㎏ of Doxy every 2 days for 2 weeks under ultraviolet light. In experiment IV, western blot was used to detect eGFP increase and decrease in skin tissues of transgenic dog under the presence or absence of Doxy. In the results of experiment I, the MFI for infected cells was rapidly increased to approximately 42.3 times after 3 day-treatment compared to pre-treatment and quickly decreased 3 days after ceasing the treatment. In experiment II, a total of 203 embryos were transferred to nine recipients and three pregnant delivered three pups (Tet-on eGFP 0, Tet-on eGFP 1, and Tet-on eGFP 2) by C-sec and Tet-on eGFP 2 among them is still alive. All cloned pups were genetically identical to the donor cell. Tet-on eGFP 2 showed an apparent in vivo eGFP expression on her body after Doxy administration in experiment III. The result of Sothern blotting showed that the transgene insertion was detected from the three cloned dogs and all organs of Tet-on eGFP 1. Experiment IV indicated that a robust eGFP expression in skin tissue of Tet-on eGFP 2 rapidly increased after Doxy treatment and gradually decreased to basal level on 9 weeks after ceasing the treatment. In conclusion, we report here for the first time an inducible transgenic system in canine species and it can stably induce the transgene expression at intended time. This study has demonstrated the capacity to generate transgenic model dog which could regulate the transgene and it would contribute to human medical research fields.

"Early Valley", is an early maturing potato cultivar with high yield potential. "Early Valley" is a clonal selection resulting from the cross between 'Suncrisp' and 'A87109-10'. It has medium plant height and light green foliage. "Early Valley" has medium flowering habit and white flowers. Tubers are smooth, yellow skin, light yellow flesh, round tuber shape, medium eye depth, and medium dormancy and good keeping quality. It has stable yield under wide range of climatic conditions. "Early Valley" is resistance to late blight, but moderately susceptible to common scab and hollow heart. This cultivar is also resistant to potato rotting at harvesting during the raining season. "Early Valley" has high level of antioxidant activity (about three times higher) and vitamin C (higher by 40%) than the 'Superior'. This cultivar has high level of tuber uniformity and capable of yielding 36.56 t/ha which is 17.07% higher than the control potato cultivar 'Superior' under optimum agronomical practices.

Bovine embryos produced by in vitro maturation, feretilization and development was examined for presevation and transfer. The fertilization medium used BO medium with 5 mM/ caffeine and 10/ heparin and adjusted to a pH of 7.2 to 7.4. The final concentration of spermatozoa was adjusted to 1 cells/ motile sperm during fertilization in vitro. At 8~10 hrs after insemination, the oocytes were transferred into CR1aa medium and cultured for 7 days. Embryos were preserved by vitrification method for transfer. When the embryos of early, blastocyst and expanded blastocyst stages were frozen-thawed, the proportions of embryos with normal morphology 83.6, 88.1 and 85.2%. (중략)

The purpose of this is to investigate the effects of vitrification in open pulled straws (OPS) on in vitro survival of porcine embryos. Blastocysts were produced by in vitro fertilization of slaughterhouse-derived, in vitro matured oocytes with frozen-thawed boar semen, and subsequent culture on granulosa cell monolayer. After frozen-thawing, embryos were culture in NCSU-23 medium with 5 mM hypotaurine, 4 mg/ BSA and 10 ng/ for 48 hrs to survival tests. When blastocysts were frozen-thawed by OPS methods, the embryos with normal morphology were 32.1, 34.5 and 38.9 % in early blastocyst, blastocyst and expanded blastocyat stages. The rates of partial damaged embryos were significantly (P<0.05) higher in early biastocysts than expanded blastocysts. In another experiment, the embryos frozen by OPS methods were cultured for 48 hrs for survival and developmental rates in vitro. The proportions of embryos hatched were 11.8, 20.2 and 33.3% in embryos frozen-thawed at stages of early blastocyst, blastocyst and expanded embryos. On the other hand, The proportions of embryo with normal morphology after culture were 23.5, 25.0 and 33.3% in embryos frozen-thawed at stages of early blastocyst, blastocyst and expanded embryos. These finding indicate the possible broader application for OPS methods that this procedure described is relatively harmless, that it can be used for blastocysts of different developmental stages.