Insect eggshell and cuticle/exoskeleton play vital roles in protecting them from natural environmental stresses. However, these chitinous cuticular extracellular matrices must be degraded at least in part during embryo hatching and molting/ecdysis periods to accommodate continuous growth all the way to the adult stage. In this study, we investigated the functional importance of groups I and II chitinases, TcCHT5 and TcCHT10, in the turnover of chitinous cuticle during both embryonic and post-embryonic development in Tribolium castaneum. RNAi and TEM analyses revealed that TcCHT10 is required for digestion of chitin in the serosal cuticle for embryo hatching as well as in the old cuticle during post-embryonic molts including larval-pupal and pupal-adult metamorphosis. TcCHT10 appears to be able to substitute for TcCHT5 in all these vital physiological events except for the pupal-adult molting in which TcCHT5 is indispensable for complete digestion of chitin in the old pupal cuticle.

한국임업진흥원은 2012년 1월 설립된 산림청 산하의 공공기관으로서 산림과학기술의 보급과 임업 및 임산업 의 고부가가치 창출을 위한 임업 서비스를 제공하고 있으며, 국내 산림분야 활성화 및 글로벌 경쟁력 확보에 기여하고 있다. 특히, 소나무재선충병으로부터 국가 산림자원 보호를 위해 소나무재선충병모니터링센터를 2016년 1월에 설치하였으며, 최근 산림병해충이 산림을 비롯하여 생활권, 농림지 등에 돌발대발생하여 국민생 활에 불편을 초래하고 재산피해를 야기함에 따라 2021년 1월에 산림병해충모니터링센터로 확대 개편하여 산림 병해충 피해 예방과 감소를 위한 다양한 역할을 수행하고 있다. 주요 역할로는 주요돌발외래 산림병해충의 예찰 및 발생피해현황 모니터링, 방제약제 등록시험 운영 및 방제품질 관리, 예찰방제 전문가 양성 교육, 대국민 홍보 등이 있으며 본 발표를 통해 현재까지 산림병해충 관리를 위한 수행 내용과 결과를 공유하고자 한다.

최근 국내에서는 꿀벌 대량소실 현상이 2022년부터 전국적으로 발생하고 있다. 우리나라 뿐 만 아니라, 전세계 적으로 양봉산업에 큰 위협이 되고 있는 봉군붕괴현상은 2016년 미국에서 세계 최초로 보고되었다. 국내에서는 2022년 민관 합동조사 결과, 이상기온, 응애, 말벌 등이 주요 원인으로 지목되었다. 대량소실 현상을 보인 양봉농 가와 정상 농가의 병원체 검출 비교 결과, 유의성있게 검출이 증가되는 병원체는 발견되지 않았다. 그러나, Tyrophagus mite, Trypanosome, Lake Sinai virus, Apis mellifera filamentous virus 등의 신종 응애, 원충 및 바이러 스 감염이 추가로 확인되었다. 국내에서 새롭게 감염이 확인된 기생충과 병원체가 대량소실, 나아가 봉군붕괴현 상에 직간접적으로 영향을 주었을 것으로 사료되며, 지속적인 조사와 연구개발을 통해 기후등 환경변화에 따른 신종 질병 검색과 대책을 마련해야 할 것이다.

Spent fuels (SFs) are stored in a storage pool after discharge from nuclear power plants. They can be transferred to for the further processes such as dry storage sites, processing plants, or disposal sites. One of important measures of SF is the burnup. Since the radioactivity of SF is strongly dependent on its burnup, the burnup of SF should be well estimated for the safe management, storage, and final disposal. Published papers about the methodology for the burnup estimation from the known activities of important radioactive sources are somewhat rare. In this study, we analyzed the dependency of the burnup on the important radiation source activities using ORIGEN-ARP, and suggested simple correlations that relate the burnup and the important source activities directly. A burnup estimation equation is suggested for PWR fuels relating burnup with total neutron source intensity (TNSI), initial enrichment, and cooling time. And three burnup estimation equations for major gamma sources, 137Cs, 134Cs, and 154Eu are also suggested.

Background: The grading of Hanwoo (Korean native cattle) is based on four economic traits, and efforts have been continuously made to improve the genetic traits associated with these traits. There is a technology to predict the expected grade based on the 4 economic genetic SNP characteristics of Korean cattle calves using single nucleotide polymorphism (SNP) technology. Selection of highly proliferative, self-renewing, and differentiating satellite cells from cattle is a key technology in the cultured meat industry. Methods: We selected the Hanwoo with high and low-scored of genomic estimated breeding value (GEBV) by using the Hanwoo 50K SNP bead chip. We then isolated the bovine satellite cells from the chuck mass. We then conducted comparative analyses of cell proliferation, immunocytochemistry, qRT-PCR at short- and long-term culture. We also analyzed the differentiation capability at short term culture. Results: Our result showed that the proliferation was significantly high at High scored GEBV (Hs-GEBV) compared to Low scored GEBV (Ls-GEBV) at short- and long-term culture. The expression levels of Pax3 were significantly higher in Hs-GEBV bovine satellite cells at long-term culture. However, there were no significant differences in the expression levels of Pax7 between Hs- and Ls-GEBV bovine satellite cells at short- and long- term culture. The expression levels of MyoG and MyHC were significantly high at Ls-GEBV bovine satellite cells. Conclusions: Our results indicated that selection of bovine satellite cells by Hanwoo 50K SNP bead chip could be effective selection methods for massive producing of satellite cells.

This study investigated ethopabate (EPB) residues in edible tissues of broiler chickens given in drinking water and established the withdrawal time (WT) of EPB in poultry tissues. Twenty-four healthy Ross broiler chickens were orally administered with EPB at the concentration of 3.8 mg/L for 14 days (EPB-1, n=24) and 15.2 mg/L for 7 days (EPB-2, n=24) through drinking water, respectively. After the drug treatment, tissue samples were collected from six broiler chickens at 0, 1, 3, and 5 days, respectively. EPB residue concentrations in poultry tissues were determined using LC-MS/MS. Correlation coefficient values ranged from 0.9980 to 0.9998, and the limits of detection and quantification (LOQ) were 0.03~0.09 and 0.1~0.3 μg/kg, respectively. Mean recoveries in muscle, liver, kidney and skin/fat tissues were 95.9~109.8, 108.7~115.3, 89.9~96.6 and 86.7~96.8%, respectively, and coefficient of variations were less than 17.11%. At the end of the drug-administration period (0 day), EPB was detected at levels under the LOQ in all tissues from both the EPB-1 and EPB-2 groups. According to the results of EPB residue in Ross broiler tissues, withdrawal periods of both EPB-1 and EPB-2 in poultry tissues were established to 0 day. In conclusion, the developed analytical method is suitable for the detection of EPB in poultry tissues, and the estimated WT of EPB in poultry tissues will contribute to ensuring the safety of Ross broiler chickens.

본 연구는 금속 인공물을 감소시키기 위한 VAT(view angle tilting)와 SEMAC(slice encoding for metal correction) 기법 적용에 따른 온도 변화 범위를 관찰하고자 하였다. 제작된 인체 모방 팬텀을 활용하였고, 검사방법으로는 임상에서 실제로 사용하고 있는 고속스핀에코(fast spin echo, FSE) 기법의 영상 파라미터들을 그대로 이용하였다. VAT 와 SEMAC 기법은 FSE와 같은 파라미터로 설정한 다음 VAT 파라미터는 100%와 SEMAC 파라미터는 25로 설정하였다. 온도 측정 방법으로는 수소원자 공명주파수전이법(proton resonance frequency shift, PRFS)기법을 활용하였으며, 광 섬유 온도계(fiber-optic sensor, FOS)로 절대 온도를 측정한 후 비교 분석하였다. 온도 변화는 SEMAC 기법에서 기존 FSE 기법 (0.28℃±0.10℃)에 비해 1.63℃±0.12℃로 약 6배 상승하였고(SEMAC-FOS = 1.59℃), VAT 기법은 약 2배 증가(VAT-FOS = 0.51℃)가 확인되었다. 특히, SEMAC 기법은 VAT 기법(VAT-FOS = 0.51℃, VAT-PRFS = 0.54℃ ±0.02℃)과 비교하여 약 3배가 증가하여 가장 높은 온도 상승이 관찰되었다. 이는 SEMAC 기법 적용 시 자기공명영상 전자파 인체 영양에 대한 안전기준을 충족하기 위해 영상 파라미터 최적화 작업의 필요성을 시사한다.

Although influencers establish their reputation and gain popularity by demonstrating expertise toward a specific topic, there is a huge potential to extend their market by tapping into different topics. Specifically, by promoting different types of product categories. However, previous studies tend to have different predictions about the success of this practice. Such that, according to the match-up hypothesis, it is unlikely that the influencer can successfully promote different product categories. On the other hand, Stereotype Content Model (SCM) suggests that influencers might be perceived as competence that overgeneralized to other domains. By conducting a survey to 302 online consumers in Indonesia, this study aims to test two competing routes toward influencer’s success in promoting product categories other than their initial expertise within the fashion context. The findings of this study revealed the primacy of match-up hypotheses, even when the influencers are perceived as competent, it does not mean that consumers are willing to follow their recommendation if it is outside their expertise domain. Only when there is an influencer-product fit, consumers are willing to accept their recommendation. However, perceived competence of the influencers can promote acceptance to follow recommendation on different product categories only when it established trust on the influencer.

Many retailers and food service providers offer programs as part of their loyalty programs in which customers are given stamps for each purchase of a qualifying product or service and redeemed for a reward once a certain number of stamps have been accumulated. We identify which stimuli in such goal-directed motivational promotions induce customers to participate in the program.

The GMT-Consortium Large Earth Finder (G-CLEF) is the first instrument for the Giant Magellan Telescope (GMT). G-CLEF is a fiber feed, optical band echelle spectrograph that is capable of extremely precise radial velocity measurement. G-CLEF Flexure Control Camera (FCC) is included as a part in G-CLEF Front End Assembly (GCFEA), which monitors the field images focused on a fiber mirror to control the flexure and the focus errors within GCFEA. FCC consists of an optical bench on which five optical components are installed. The order of the optical train is: a collimator, neutral density filters, a focus analyzer, a reimager and a detector (Andor iKon-L 936 CCD camera). The collimator consists of a triplet lens and receives the beam reflected by a fiber mirror. The neutral density filters make it possible a broad range star brightness as a target or a guide. The focus analyzer is used to measure a focus offset. The reimager focuses the beam from the collimator onto the CCD detector focal plane. The detector module includes a linear translator and a field de-rotator. We performed thermoelastic stress analysis for lenses and their mounts to confirm the physical safety of the lens materials. We also conducted the global structure analysis for various gravitational orientations to verify the image stability requirement during the operation of the telescope and the instrument. In this article, we present the opto-mechanical detailed design of G-CLEF FCC and describe the consequence of the numerical finite element analyses for the design.

Background: Swallowing disorder is a common disease that can cause various complications such as malnutrition, dehydration, aspiration pneumonia, and poor quality of life, and can affect people of all ages, from infants to the elderly. Swallowing disorder rehabilitation is a complex process involving various skills such as exercise, diet modification, and posture adjustment. This study implements a rehab program for swallowing disorders using the Kinesio Tapping technique. Design: Prospective pilot case study. Objectives: To improve laryngeal detachment by providing external support to the muscles involved in swallowing using kinesio taping for conventional complex swallowing disorder rehabilitation. Methods: The study included 16 participants who were diagnosed with swallowing difficulties after a stroke and admitted to a rehabilitation ward in Ulsan. The average age of the participants, consisting of 9 males and 7 females, was 69.0 years (SD=1.23) and 64.0 years (SD=1.45), respectively. The stroke types were hemorrhagic and infarction, with onset periods ranging from 6 to 12 months (SD=1.11) in all participants. Each participant received a video fluoroscopy study (VFSS) for six months using the taping method developed by the research team. Kinesio taping techniques were developed by two professional physical therapists and one occupational therapist based on anatomical movements. Results: Significant differences were observed in self-training on the bed after the rehabilitation program compared to the initial period Kinesio taping can help improve laryngeal dislocation and reduce the risk of aspiration by providing external support to the muscles involved in swallowing. However, the effectiveness and optimal application techniques of kinesio taping in the rehabilitation of swallowing disorders are not yet clear. Conclusion: The results of this study could lead to the development of a new treatment protocol that incorporates kinesio taping as a standard therapy for the rehabilitation of swallowing disorders in bedridden patients.

With about 80% of the global economy expected to shift to the global market by 2030, exports of reverse direct purchase products, in which foreign consumers purchase products from online shopping malls in Korea, are growing 55% annually. As of 2021, sales of reverse direct purchases in South Korea increased 50.6% from the previous year, surpassing 40 million. In order for domestic SMEs(Small and medium sized enterprises) to enter overseas markets, it is important to come up with export strategies based on various market analysis information, but for domestic small and medium-sized sellers, entry barriers are high, such as lack of information on overseas markets and difficulty in selecting local preferred products and determining competitive sales prices. This study develops an AI-based product recommendation and sales price estimation model to collect and analyze global shopping malls and product trends to provide marketing information that presents promising and appropriate product sales prices to small and medium-sized sellers who have difficulty collecting global market information. The product recommendation model is based on the LTR (Learning To Rank) methodology. As a result of comparing performance with nDCG, the Pair-wise-based XGBoost-LambdaMART Model was measured to be excellent. The sales price estimation model uses a regression algorithm. According to the R-Squared value, the Light Gradient Boosting Machine performs best in this model.