Background : This study was carried out to provide the basic data for selection of excellent resources and true seeds of southern medicinal plant (Camellia japonica). Methods and Results : The germination rate at room temperature and 4℃ dry storage did not show a tendency according to seed weight. The germination rate of 4℃ wet sand storage increased with seed weight but showed very low germination rate at 120 days of storage. The germination rate at 4℃ wet filter paper storage was higher than 80% in 60 days, 90 days and 120 days storage, and there was no difference in germination rate with seed weight. The mean number of germination days was about 30 days in average at room temperature and 4℃ dry storage, but wet storage (sand, filter paper) was short as about 13 days. Conclusions : The seeding of Camellia japonica seeds (last autumn harvest) in the spring of the next year was evaluated as the most efficient way to 4℃ wet filter paper storage

Background : This text was conducted to investigate the fruit type of Camellia japonica in ko rea. Methods and Results : In fruit of Camellia japonica, For fruit length of 24.1～33.0mm was 6 3.7%, and width of 30.1～39.0mm was 60.2%, and weight of 10.1～25.0g was 62.1%. Fruit was near in circle shape, because length/width ratio 0.71～1.00 indicate 79.6%. Seed number of per fruit by weight was also tends to be which is much as fruit is heavy. In correlation b etween each of fruit character, Fruit width showed a positive correlation with the fruit length, fruit weight, seed number. Also, for seed number, the correlation coefficient is the fruit weigh t (0.643**), fruit width (0.640**) was significantly more than the length (0.532**). Conclusions : Camellia japonica was very various size, shape of fruit and seed number of per fruit.

The flower buds of Sophora japonica L (SF), as a well-known traditional Chinese medicinal herb, have been used to treat bleeding-related disorders such as hematochezia, hemorrhoidal bleeding, dysfunctional uterine bleeding, and diarrhea. However, no specific anti-cancer effect and its molecular mechanism of SF have been described. Thus, we performed in vitro study to investigate if treatment of SF affects activating transcription factor 3 (ATF3) expression and ATF3-mediated apoptosis in human colorectal cancer cells. The effects of SF on cell viability and apoptosis were measured by MTT assay and Western blot analysis against cleaved poly (ADP-ribose) polymerase (PARP). ATF3 activation induced by SF was evaluated using Western blot analysis, RT-PCR and ATF3 promoter assay. SF treatment caused decrease of cell viability and increase of apoptosis in a dose-dependent manner in HCT116 and SW480 cells. Exposure of SF activated the levels of ATF3 protein and mRNA via transcriptional regulation in HCT116 and SW480 cells. Inhibition of extracellular signal-regulated kinases (ERK) 1/2 by PD98059 and p38 by SB203580 attenuated SF-induced ATF3 expression and transcriptional activation. Ectopic ATF3 overexpression accelerated SF-induced cleavage of PARP. These findings suggest that SF-mediated apoptosis may be the result of ATF3 expression through ERK1/2 and p38-mediated transcriptional activation.

Reuse, recycling and recovery (3R) of industrial wastes are common and encouraging in Korea. Industrial symbiosis is one of typical methods for implementing 3R and has been defined as engaging “traditionally separate industries in a collective approach to competitive advantage involving physical exchange of materials, energy, water, and by-products”. The keys to industrial symbiosis are collaboration and the synergistic possibilities offered by geographic proximity. Recently, several countries in East Asia such as Taiwan, South Korea, Japan and China are promoting ‘designed’ symbiosis networks in various industrial complexes based on their national Eco-industrial Park (EIP) demonstration programs. Despite the recently promoted EIP initiatives worldwide, only very few studies furnish the operational and functional details of ‘designed’ industrial symbiosis networks, starting from the planning to implementation stage. The production of terephthalic acid (TPA) in Korea is 6.5 million ton based on 2011. The production of waste generated in the process of TPA is about 15,000 ton/yr and included useful materials like residue TPA as well as isophthalic acid (iPA), benzoic acid (BA) and p-toluic acid (p-TL). Until now, TPA waste was incinerated due to high caloric value. This study was conducted for recovery of valuable materials from industrial waste and utilization of recovered material as an example of Korean EIP. In this study, the experiment was performed to separate above four materials from the waste by solvent (methanol and water) extraction. The solubility of TPA (0.1g/100g) for methanol is relatively low compared to other materials. Also the solubility of BA (68g/L at 95℃) and p-TL (11.6g/L at 98℃) for water is relatively high in comparison with other materials. Finally, BA was separated from p-TL by molecular distillation system. The purity of recovered materials was analyzed by ultra-high performance liquid chromatography (Waters Xevo TQ system). The recovery rate and purity of BA is 90% and 93%. The recovered BA could be recycled the companies which are produced plastic plasticizer and paints.

Prevalence of the coronary artery anomaly is approximately 1% of the population who undergo coronary angiography. The anomalous origin of the right coronary artery (RCA) as a branch of the left anterior descending artery (LAD) is a very rare variation of single coronary artery. The anatomic variation has no clinical significance. However, some patterns of congenital coronary artery anomalies can cause clinical manifestations of myocardial ischemia, reducing myocardial perfusion. We report on a case of a 78-year-old man who had anomalous RCA arising from the proximal part of the LAD, which probably caused chest pain.

Everolimus is a secondary medication for metastatic renal cell carcinoma when vascular endothelial growth factor inhibitor treatment fails. Everolimus is reported to cause a variety of metabolic complications but complications in the eye are rare. Although serious eye complications have not been reported, secondary complications may result from the metabolic complications. We observed a case in which central retinal vein occlusion occurred, probably caused by metabolic complications following Everolimus treatment of RCC. We report this case.

Respiratory distress resulting from a neuromuscular disorder is often misdiagnosed and not managed properly until development of severe respiratory failure. Amyotrophic lateral sclerosis (ALS) is a rare but archetypical motor neuron disease. Most cases manifest as weakness, muscular atrophy, and progressive paralysis of the limbs. Late stage of the disease is characterized by respiratory failure due to respiratory muscle weakness. In a few cases, 3% of patients, respiratory failure is the initial presentation, preceding other symptoms. We report on a case of ALS accompanied by acute respiratory failure and weaning failure, with a review of the literature.

Regarding carotenoids content, the genetic basis, heritability and combining ability in six red pepper inbred lines were investigated using full diallel crosses. Both additive and non-additive gene actions govern inheritance of carotenoids content. The mean square of array through variance and covariance analysis (Wr-Vr) was insignificant, which suggest that inbred lines involved in diallel cross may have no epistatic effects. The Vr/Wr graph revealed the influence of partial dominant gene action towards low carotenoids content and the absence of non-allelic interaction. The H2 component was smaller than the H1 and the [H2/4H1] component was 0.187 less than 0.25, indicating unequal proportion of positive and negative alleles in the parents. The estimates of broad and narrow sense heritability for carotenoids content were 0.956 and 0.832, respectively. The variance of general combining ability (GCA) was relatively higher than that of specific combining ability (SCA), which implied that the additive gene effects were predominant as compared to both dominant and epistatic effects for the accumulation of carotenoids in this genetic population. The values of GCA of ‘62024L1’ and ‘62067L2’ were higher than those of the other parents. These 2 inbred lines, therefore, can be considered as useful breeding materials to enhance fruit carotenoids content in other red pepper varieties.

Dandelion has been widely utilized for medicinal and edible purposes. This research was conducted to evaluate the effect of supplemental LED (light-emitting diode) light on germination, growth characteristics and anthocyanin content of dandelion (Taraxacum officinale) seedling using LED blue (460 nm), red (660 nm, R), blue + red (B:R=6:4) and fluorescent lamp light treatment. By LED illumination to T. officinale seed germination speed was delayed, and germination rate was the highest in the fluorescent light. The growth characteristics (plant height, number of leaves, root length and fresh weight) were greatly influenced by supplemental LED light compared with control treatment, and the growth promotion was the most effective in the red LED illumination. After 60 days of red and mixed LED light treatments, anthocyanin content of dandelion plants was significantly changed. The anthocyanin content was increased by 12~19 mg/100 g under the red LED and the mixed light conditions compared with the control and the blue LED. Results indicate that illumination with red and mix LEDs, compared with other light treatments, is beneficial for promotion of growth and anthocyanin content in dandelion.

The bulb storability of eight red and ten yellow onion (Allium cepa L.) cultivars grown primarily in Korea was evaluated. During storage, sprouting occurred earlier in red cultivars than in yellow cultivars. In addition, the ratio of sprouted to unsprouted bulbs increased more rapidly in red cultivars than in yellow cultivars. However, not all yellow cultivars had strong storabilities. Bulb storability of three yellow cultivars was as poor as that of red cultivars, suggesting only a slight possibility of a pleiotropic effect of color and bulb storability. Meanwhile, F3 lines of a red cultivar selected based on stronger storabilities showed intermediate storabilities between those of the red and yellow cultivars, implying that strong storability could be obtained by successive selection. In contrast, F1 hybrids from crosses between yellow and red breeding lines showed poor storability compared with the yellow cultivars, indicating that poor storability may be dominant over strong storability. The relationships between color and other traits that potentially affect storability, such as fresh and dry weight, water content, and firmness of bulbs, were evaluated. No significant differences in these traits were observed between the two colors, although the water content of yellow cultivars and the dry weight of red cultivars were slightly higher than their counterparts. Correlation analyses between bulb storability and other traits, including weight, water content, and firmness, also showed no significant correlations. In this study, no correlations were identified between bulb color, storability, and other traits. However, based on these results, red cultivars with stronger storabilities could be developed through successive selection of bulbs with stronger storabilities.

Triterpene saponins (ginsenosides) are main bioactive compounds in Panax ginseng. From mevalonic acid pathway, the isoprene units was synthesized that is precusor of triterpene saponin. This study provides the complete nucleotide sequence of the full-length cDNA involved in mevalonic acid biosynthesis pathway. We had a full-length cDNA sequences as followed; HMGS (1944bp, 1407bp ORF), HMGR (2120bp,1728bp ORF) and MDD (1760bp, 1263bp ORF) with partial poly (A). We were used real time PCR and High Performance Liquid Chromatography (HPLC) to analysis the expression level of the gene involved in MVA pathway and to ginsenoside contents, respectively. All cloned genes are constitutively expressed in all tested tissues but at different levels with the highest expression in fine roots. But ginsenoside contents is highest in leaf > fine root> lateral root > red berry> main root > petiol > stem. After treantment of methyl Jasmonate (MeJA), MVA pathway genes are highly expressed and ginsenoside contents was high at 72h.

Methyl jasmonate(MeJA) treatment promotes the plant secondary, especially triterpene saponin which is main compounds in Platycodon grandiflorum. We were performed 5,760 expressed sequence tags from MeJA treated hairy root. Total number of ESTs are 2,536 (811 contigs and 1,725 singletons) among them 2,299 are pass the annotation. The most abundant transcript were encoding fatty acid desaturase (FAD) and lots of secondary metabolite transcript were observed. Four MVA pathway genes (HMGS, HMGR, MK, MDD) and one MEP pathway gene (IDI) are identified. The unigene were classified into three major functional categories by standard GO (Gene Ontology) - cellular component, molecular function and biological process. In this study we were cloned four genes from ESTs : HMGS (1401bp ORF), MK (1167bp ORF), MDD (1254bp ORF), IDI (909bp ORF)