초 록 잠자의 중요한 병인 역병(Phytopthora infestans)은 우리나라의 주요씨감자 생산지대인 중부 고랭지대에서 매년 심한 피해를 초래하고 있는데 만일 포장에 역병이 처음 관찰된 후 약제를 살포치 않으면 식물체의 지상부는 14일 이내에 완전고사 하게 된다. 규칙적인 약제살포로 역병방제가 가능하지만 더욱이 역병발생예보에 따라 적기에 약제살포하므로써 방제효과는 물론 생산비를 절감할 수 있게 된다. 본 시험은 1970-1977년 8년간에 걸쳐 대관령의 기상상태와 역병발생 상황을 조사한바 7일 평균기온과 7일 적산강수량을 사용한 Hyre씨의 이동그라프(Moving graph)법에 의한 예보는 정확성이 높지 않았으나 여기에 상대습도와 7일 평균최저기온을 첨가하므로서 비교적 "Moving graph" 법에 의한 예보의 정확성을 높일 수가 있었다. 그러나 아직도 보다 정확한 역병예찰을 위하여 계속적인 조사시험이 필요하다고 생각된다. 또한 약제를 살포하는 곳에서의 역병에 감수성인 품종의 수량은 역병발생정도와 강우량과 밀접한 관계가 있었으며 특히 대관령 지방에서 감자 파종 후 역병발생까지의 일수와 파종 후 90일 수량으로 그 해의 씨감자의 수확양과 공급량을 미리 예측할 수 있게 할 것으로 사료된다.

This report describes the inception, development and extensive use over 30 years of elliptical polarimeters at the Uni\-versity of Pennsylvania. The initial Mark I polarimeter design utilized oriented retarder plates and a calcite Foster-Clarke prism as the analyzer. The Mark I polarimeter was used on the Kitt Peak 0.9 m in 1969-70 to accomplish a survey of ap\-proximately 70 objects before the device was relocated to the 0.72 m reflector at the Flower and Cook Observatory. Suc\-cessive generations of automation and improvements included the early-80’s optical redesign to utilize a photoelastic modulated wave plate and an Ithaco lock-in amplifier–the photoelastic modulating polarimeter. The final design in 2000 concluded with a fully remote operable device. The legacy of the polarimetric programs includes studies of close binaries, pulsating hot stars, and luminous late-type variables.

Crop protection strategies in organic horticulture aim to prevent insect pest and plant disease problems through utilization of non-chemical based control means. In order to develop a model for management of plant diseases and insects in organic cucumber cultivation, we compared efficacies between chemical pesticide spraying system and biological control means in semi-forcing and retarding cucumber cultivation during 2005 and 2006. Conventional chemical spray program using various chemical pesticides was applied 5 - 10 days intervals, while two different non-chemical pesticide application programs using two formulated biopesticides Topseed™ and Q-fect™， Suncho™, and Sangsungje™(biocontrol agents 1) and using egg-yolk and cooking oil(EYCO)， Bordeaux mixture, Suncho™, and ‘Sangsungje™(biocontrol agents 2) were applied 5 - 7 days intervals during entire cucumber cultivation period. Efficacy of both biocontrol agents programs was effective to comparable to conventional chemical pesitice spray program to control plant diseases such as powdery mildew and downy mildew as well as insect pests such as aphids and thrips which are known as major threats in cucumber organic cultivation. In this study, we established and evaluated an effective and economic crop protection strategy using various biological resources can be used to control plant diseases and pests simultaneously in organic cucumber cultivation field.

The development of humanized culture system of human embryonic stem cells (hESCs) hold promise for therapeutic applications. However, conventional culture system contain animal-derived components such as fetal bovine serum and mouse embryonic fibroblasts that bear a risk of transmitting non-human pathogens and incorporation of non-human immunogenic molecules to hESCs. In this study, we developed an efficient xeno-free hESCs culture system using humanized materials, the CELLstartTM, human foreskin feeder and xeno-free medium containing knockOutTM SR XenoFree (XF-medium) without animal-derived material. The hESCs were gradually adapted to the XF-medium; 25:75, 50:50, 75:25 and 100:0. Two karyotypically normal hESC lines, SNUhES4 and H1, were used for the experiments of xeno-free culture condition. The attachment rates at xeno-free culture system were 52.6±12.4%, 67.0±16.6%, 59.0±13.9%, 28.3±2.9% in SNUhES4, 79.3±5.4%, 53.8±20.9%, 69.4 ±6.4%, 59.8±12.6% in H1 and the spontaneous differentiation rates were 42.2±12.7%, 31.4±2.9%, 40.8±14.5%, 55.2±35.5% in SNUhES4, 35.6±8.5%, 36.4±13.5%, 48.4±7.8%, 80.1±6.0% in H1 in the first four passage. Although the attachment rates were low and the spontaneous differentiation rates were high compared to that of conventional system in the early passages using this humanized culture condition, hESCs in this culture condition were found to maintain hESC characterizations; morphology, expression of cell surface markers and stable karyotype. Our results indicate that simplified compositions of humanized culture system can be applicable to the further optimization for a xeno-free culture of hESCs without the loss of pluripotency and contamination from xenogenic sources.

Controllable transgenic expression systems in transgenic animal model are valuable to the development of therapeutic approaches in human medical fields. The aim of this study was to 1) produce a transgenic cloned dog using inducible tetracycline vector system, and 2) investigate whether the transgenic cloned dog could be induced the transgene expression using doxycycline (Doxy). Canine fetal fibroblasts were infected with retroviral vectors designed to express the enhanced green fluorescent protein (eGFP) gene under the control of tetracycline-inducible promoter. For somatic cell nuclear transfer (SCNT), nucleus of an in vivo matured oocyte was removed and an eGFP expressed cell cultured with 1 ㎍/㎖ of Doxy was injected. After electrical fusion and chemical activation, the reconstructed embryos were transferred to a recipient and pregnancy diagnosis was performed by ultrasonography. Experiment I evaluated the mean fluorescence intensity (MFI) of infected cells while the cells were cultured in the presence of 1 ㎍/㎖ of Doxy for 5 days, and then in the absence of Doxy for 7 days using fluorescence-activated cell sorter. Experiment II was designed to produce an eGFP controllable transgenic cloned dog via SCNT. For verification of transgenic dog, experiment III was performed Southern Blot analysis and observation in vivo regulation of eGFP expression in the cloned dog treated with 100 ㎎/㎏ of Doxy every 2 days for 2 weeks under ultraviolet light. In experiment IV, western blot was used to detect eGFP increase and decrease in skin tissues of transgenic dog under the presence or absence of Doxy. In the results of experiment I, the MFI for infected cells was rapidly increased to approximately 42.3 times after 3 day-treatment compared to pre-treatment and quickly decreased 3 days after ceasing the treatment. In experiment II, a total of 203 embryos were transferred to nine recipients and three pregnant delivered three pups (Tet-on eGFP 0, Tet-on eGFP 1, and Tet-on eGFP 2) by C-sec and Tet-on eGFP 2 among them is still alive. All cloned pups were genetically identical to the donor cell. Tet-on eGFP 2 showed an apparent in vivo eGFP expression on her body after Doxy administration in experiment III. The result of Sothern blotting showed that the transgene insertion was detected from the three cloned dogs and all organs of Tet-on eGFP 1. Experiment IV indicated that a robust eGFP expression in skin tissue of Tet-on eGFP 2 rapidly increased after Doxy treatment and gradually decreased to basal level on 9 weeks after ceasing the treatment. In conclusion, we report here for the first time an inducible transgenic system in canine species and it can stably induce the transgene expression at intended time. This study has demonstrated the capacity to generate transgenic model dog which could regulate the transgene and it would contribute to human medical research fields.