probiotics strains promoting the health are a collection of microorganisms that improve or restore microbial populations in the intestines. In this study, Leuconostoc probiotics was isolated from fermented gimchi and identified. Angelica dahurica, containing abundantly antioxidant activity, imperator, is a wildly grown species of angelica native. Before fermentation, total phenolics compound were 48.83 ± 4.9 GAE mg /g in the Angelica dahurica extract. After fermentation total phenolic compounds were 97.7 ± 12.6 GAE mg/g. The total amount of phenol in the fermented product was 30.2% higher than that before fermentation. The total flavonoid content before fermentation was 9.86 ± 4.3 mg / g and the total flavonoid content was 37.17 ± 7.4 mg/g after fermentation, which was 82.3% higher than before fermentation. The DPPH radical scavenging activity, superoxide radical scavenging activity, hydroxy radical scavenging activity and Fe++ chelating antioxidative activity of the Angelica dahurica extract were 41.6 ± 7.1%, 65.7 ± 8.4%, 55.26 ± 9.4% and 17.5 ± 4.6%, respectively. After fermentation, they were 60.3 ± 12.6%, 78.8 ± 8.3%, 56.9 ± 4.9% and 36.6 ± 8.9%, respectively. Therefore, the present study suggests that the fermentation using the probiotics strain of the Angelica dahurica extract can be used as a functional health food and cosmetic material with increased antioxidant capacity.

Hairy root culture of ginseng is industrially prospected because the cultivation period of ginseng is relatively long. In this study, the effect of medium concentration and sucrose concentration on hairy root culture of ginseng was evaluated. The optimization of ginseng hairy roots transformed by Agrobacterium rhizogene were performed liquid medium. The MS(Murashinge & Skoog basal medium) concentration was selected with 1/2 strength MS and the optimal sucrose concentration was determined at 2-3%(w/v). At the optimum culture condition, The yield (the ratio of weight of grown hairy root cultures to weight of fresh ginseng hairy roots) and production rate of ginseng root were 19.42 times and 5.73 g/l-day. The major ginsenosides were Rb group, Re and Rg1. The produced total ginsenoside content in the solid medium was 9.87 (mg/g) and increased 1.34 times in the liquid medium (13.23 mg/g). In solid culture, the contents of ginsenosides Rb, Re and Rg1 were 2.14, 3.65 and 1.87 mg/g, respectively. In liquid culture, the contents of ginsenosides Rb, Re and Rg1 were 3.54, 4.12 and 2.63 mg/g, respectively.

본 연구는 국내에서 절화국화를 재배하고 있는 27개 농가를 선정하여 시설과 재배현황, 수확 후 관리 및 유통 실태를 조사하였다. 재배농가들의 60%는 PE필름 비닐하우스에서 토양 관비재배 방법으로 절화국화를 재배하고 있었으며, 전남과 부산은 스탠다드 국화를 주로 재배하고 있었다. 자가채취 또는 업체에서 구매한 삽수를 플러그 육묘하여 발근묘를 생산하고 있었으며, 전남지역에서는 주로 재배상에 직접 삽목하고 있었다. 절화국화를 수확한 후 전처리로 열탕처리 또는 수돗물로 물올림을 실시하는 농가가 66.6%로 나타났다. 예냉은 70.4% 농가에서 실시하지 않았으며, 예냉하는 농가들은 주로 2~4℃에서 실시하고 있었다. 절화국화를 수확한 후 유통 물량을 조절하기 위해 70.4%의 농가에서 저장을 하고 있었으며, 2~4℃에서 48시간 이상 저장하고 있었다. 절화국화를 출하 전에 주로 개인별로 선별하고 있었으며, 일부 수출 농가에서만 공동선별을 하고 있었다. 유통에 있어서 모든 농가들은 국내로 출하하고 있었으며, 이들 농가 중 44.4%는 수출을 겸하고 있었다. 국내 시장으로 출하하는 경우, 63.0%의 농가에서 화훼경매장을 이용하고 있었다.

Alcohol intake is known to affect various organs in the human body, causing reduction of salivation in the oral cavity. Hypo-salivation effect of alcohol is a common feature, but the mechanism in salivary glands is still poorly studied. Therefore, in this study, the changes in salivary secretion and water channel protein (aquaporin5, AQP5) in salivary glands of mice were investigated after ethanol administration. Animals were divided in to 4 groups with the control, 4 g/kg ethanol, 8 g/kg ethanol and 16 g/kg ethanol administration groups. One hour after ethanol administration, saliva was collected from the oral cavity, and the animals were killed and parotid and submandibular glands were extracted to analyze the histopathology, AQP5 immunihistochemistry and AQP5 protein level. According to the results, the salivation rate decreased irrespective of the ethanol dose in mice, and viscosities increased with increase in ethanol dose. However, there were no pathological changes in parotid and submandibular glands due to ethanol administration. Expression of AQP5 in parotid and submandibular glands decreased with increase ethanol administration These results indicate that the reduction of salivary secretion due to acute alcohol intake is closely related to decrease of the water channel protein such as AQP5 in parotid glands and submandibular glands, rather than the damage of salivary glands.

Purpose The Jeju black cattle are a type of traditional Korean native cattle with a characteristic black fur that covers the entire body. The Jeju black cattle are rare breed and designated as national natural monuments in 2013. It is necessary to improve reproductive techniques for the preservation and proliferation of Jeju black cattle. Methionine acts as a precursor amino acid for glutathione in protection of cells from oxidative damage, and plays a vital role in detoxification. Low sperm motility causes infertility because when sperm do not have progressive motility, it was unable to reach the ovum. The purpose on this study was to investigate whether intake of L-methionine improves sperm motility and fertility. Materials and Methods 6 Jeju black cattles over 10 years of age are raised in Subtropical Livestock Research Institute, National Institute of Animal Science, RDA. 6 Jeju black cattles was fed with L-methionine with 10g/day during 6 weeks. Semen of Jeju black cattle were collected by artificial vaginal technique. Collected sperm was diluted with AndroMed® extender and cryopreserved in 0.5 ml French straws. Fresh and freeze-thawed sperm viability and motility were evaluated by CASA. Results The viability and progressive motility of fresh spermatozoa(live spermatozoa means: from 74.41% to 80.22%, progressive motility means: from 80.60% to 95.40%) and freeze-thawed spermatozoa(live spermatozoa means: from 51.26% to 62.05%, progressive motility: from 29.68% to 45.44%) incresed after L-methionine ingestion. Conclusion The intake of L-methionine in genetically valuable cattle over 10 years of age seems to be useful for improving sperm function.

This summer, Korea had to deal with extreme heat wave. In this research, fruit flies (Drosophila) were used to analyze the behavior of insects at a high temperature. For the experiment, three male and female fruit flies were put in each of 4 media vials. One vial was incubated at 25°C, the average temperature in August. In three vials, fruit flies were incubated in each 28°C, 30°C, and 32°C for the experiment. The breeding coefficient and death rate were calculated by counting the number of imago fruit flies in each vial and how many of them died. Also, we observed various behavioral differences. The air speed velocity of fruit flies was measured to compare the difference in behavior of fruit flies. Finally, size of them were observed by dissecting microscope. From this research, we can realize the correlation between global warming and the ecosystem of insects.

The purpose of this study was to analyze the effects of manual therapy on lower extremity alignment in pelvic malalignment. The subjects were 20 adults with pelvic malalignment. They were divided into two groups: manual therapy group (n=10) and stretching exercise group (n=10). Each group performed the intervention two times per week for 4 weeks. The lower extremity alignment was measured by pelvic deviation, functional leg length inequality, and plantar pressure distribution, which were measured between pre- and post-test. In the result of pelvic deviation, there was a significant difference between the pre- and post-test of the manual therapy group and stretching exercise group. In the result of the functional leg length inequality, there was a significant difference between the pre- and post- test of the manual therapy group. In the result of plantar pressure distribution, there was a significant difference between the pre- and post- test of the manual therapy group. These findings suggest manual therapy improves the pelvic deviation, functional leg length inequality, and plantar pressure distribution in the pelvic malalignment.

As information technology advanced, customers’ service consumption process heavily shifted to the online environment, particularly social media and mobile. A new topic, customer engagement, has emerged along with the fast advancement of social media. Customer engagement refers to a type of customer behavior that is beyond purchase such as spreading word-of-mouth, providing recommendations to family and friends, interacting with fellow customers, and writing online reviews (Bijmolt et al., 2010; Verhoef, Reinartz, & Krafft, 2010). This study introduces the Flow theory, which is especially important to understand consumers’ online experiences, with the intention to shed light on how to better engage consumers in the hospitality industry (Bilgihan, Okumus, Nusair, & Bujisic, 2014; Hoffman & Novak, 2009). The purpose of this study is to examine the antecedents of flow and further investigate its influence on positive attitude and continuance intention among restaurant social media users. This study specifically examines restaurant customers who use social media through their smart phones in searching information and sharing experiences with others. A self-administered questionnaire was developed and a Structure equational modeling (SEM) was employed to test the proposed hypotheses. Study results support the hypotheses, indicating the importance of creating flow to increase customer engagement. Academically, this study contributes to the limited body of literature on flow experience and customer engagement in the hospitality context. Additionally, it provides practical insights for hospitality marketers on how to gain competitive advantages by strategically managing customer engagement on social media marketing through flow.

Recently, there is an increasing the pavement distresses such as rutting with an increase in heavy vehicles on the road in Mongolia. Rutting is the longitudinal depression in the wheel path in asphalt pavements and it causes a hydroplaning and severe safety concern for users. This study aims to develop paving material that can prevent rutting on the road pavement by improving the durability of the asphalt mixture in Mongolia. Therefore, this study was carried out using the technique of reinforcing the material by adding fibers to conventional asphalt mixture. Fibers have been used to reinforce various materials for many decades in various parts of the world. It is generally understood that asphalt is strong in compression and weak in tension. Adding fibers with high tensile strength can help increase the strength of a mixture[1]. A mixture of glass fibers was used in this study to evaluate the performance characteristics. In coordination with the City of Ulaanbaatar, The test section selected in this study was Peace Avenue in Ulaanbaatar. The test section was a bus lane with severe rutting by heavy vehicles. The designated road test section performed cutting and overlay using five asphalt mixtures: Glass Fiber-reinforced Asphalt, Hot Mix Asphalt(10mm, 19mm), Polymer Modify Asphalt(2 types). The performance survey was conducted after the summer. As a result, No noticeable cracks were observed in glass-reinforced mixture section and the rut-depth of the glass-reinforced mixture is lower than other mixtures[2].

Recently, Cambodia has been investing a lot of money in the construction and maintenance of roads, which are social infrastructures. However, damage to the existing pavement is accelerating due to the old age of the road pavement, poor drainage facilities and increase in heavy traffic. To solve this problem, a fundamental solution such as a high-performance asphalt mixture is required to extend the life of road pavement. In this study, a high performance glass fiber reinforced asphalt mixture developed in Korea was applied to Cambodia. Prior to field application, Marshall stability tests were performed on glass fiber reinforced asphalt mixtures, SBS modified asphalt mixtures and asphalt mixtures commonly used in Cambodia. The Marshall stability test showed that the glass fiber reinforced asphalt mixture and the SBS modified asphalt mixture had the same strength (about 1.3 times higher strength than the usual asphalt mixture). In addition, the test construction was carried out on the National Highway 2 of Cambodia for the evaluation of the performance of the three mixtures. In the future, the long-term performance evaluation of each mixture will be conducted through follow-up survey of the test construction area.