Recent observations have shown that coronal magnetic fields in the northern (southern) hemisphere tend to have negative (positive) magnetic helicity. There has been controversy as to whether this hemispheric pattern is of surface or sub-surface origin. A number of studies have focused on clarifying the effect of the surface differential rotation on the change of magnetic helicity in the corona. Meanwhile, recent observational studies reported the existence of transient shear flows in active regions that can feed magnetic helicity to the corona at a much higher rate than the differential rotation does. Here we propose that such transient shear flows may be driven by the torque produced by either the axial or radial expansion of the coronal segment of a twisted flux tube that is rooted deeply below the surface. We have derived a simple relation between the coronal expansion parameter and the amount of helicity transferred via shear flows. To demonstrate our proposition, we have inspected Yohkoh soft X-ray images of NOAA 8668 in which strong shear flows were observed. As a result, we found that the expansion of magnetic fields really took place in the corona while transient shear flows were observed in the photosphere, and the amount of magnetic helicity change due to the transient shear flows is quantitatively consistent with the observed expansion of coronal magnetic fields. The transient shear flows hence may be understood as an observable manifestation of the pumping of magnetic helicity out of the interior portions of the field lines driven by the expansion of coronal parts as was originally proposed by Parker (1974).

In this paper we present a methodology to derive the temporal change of the magnetic shear angle from a series of vector magnetograms, with a high time cadence. This method looks for the minimum change of the shear angle between a pair of magnetograms, free from the 180° ambiguity, and then accumulates this change over many successive pairs to derive the temporal change of magnetic shear. This methodology will work well if only the successive magnetograms occurred in an active region are well aligned and its helicity sign is reasonably determined. We have applied this methodology to a set of vector magnetograms of NOAA Active Region 9661 on October 19, 2001 by the new digital magnetograph at the Big Bear Solar Observatory (BBSO). For this work we considered well aligned magnetograms whose cross-correlation values are larger than 0.95. As a result, we have confirmed the recent report of Wang et al. that there was the abrupt shear change associated with the X1.6 flare. It is also demonstrated that the shear change map can be an useful tool to highlight the local areas that experienced the abrupt shear change. Finally, we suggest that this observation should be a direct support of the emergence of sheared magnetic fields.

Recently, we have set up a new digital CCD camera system, MicroMax YHS-1300 manufactured by Roper Scientific for Hα observation by Solar Flare Telescope at Bohyunsan Optical Astronomy Observatory. It has a 12 bit dynamic range, a pixel number of 1300×1030, a thermoelectric cooler, and an electric shutter. Its readout speed is about 3 frames per second and the dark current is about 0.05 e-/p/s at -10°. We have made a system performance test by confirming the system linearity, system gain, and system noise that its specification requires. We have also developed a data acquisition software which connects a digital camera con-troller to a PC and acquires Hα images via Microsoft Visual C++ 6.0 under Windows 98. Comparisons of high quality Hα images of AR 9169 and AR 9283 obtained from SOFT with the corresponding images from Learmonth Solar Observatory in Australia confirm that our Hα digital observational system is performed properly. Finally, we present a set of Hα images taken from a two ribbon flare occurred in AR 9283.

We have developed a near real-time flare alerting system which (1) downloads the latest GOES-l0 1-8 Å X-ray flux 1-min data by an automated ftp program and shell scripts, (2) produces a beep sound in a simple IDL widget program when the flux is larger than a critical value, and (3) makes it possible to do a wireless alerting by a set of portable transceivers. Thanks to the system, we have made successful Ha flare observations by the Solar Flare Telescope in Bohyunsan Optical Astronomy Observatory. This system is expected to be helpful for ground-based flare observers.

Nonpotential characteristics of magnetic fields in AR 5747 are examined using Mees Solar Observatory magnetograms taken on Oct. 20, 1989 to Oct. 22, 1989. The active region showed such violent flaring activities during the observational span that strong X-ray flares took place including a 2B/X3 flare. The magnetogram data were obtained by the Haleakala Stokes Polarimeter which provides simultaneous Stokes profiles of the Fe I doublet 6301.5 and 6302.5. A nonlinear least square method was adopted to derive the magnetic field vectors from the observed Stokes profiles and a multi-step ambiguity solution method was employed to resolve the 180° ambiguity. From the ambiguity-resolved vector magnetograms, we have derived a set of physical quantities characterizing the field configuration, which are magnetic flux, vertical current density, magnetic shear angle, angular shear, magnetic free energy density, a measure of magnetic field discontinuity MAD and linear force-free coefficient. Our results show that (1) magnetic nonpotentiality is concentrated near the inversion line in the flaring sites, (2) all the physical parameters decreased with time, which may imply that the active region was in a relaxation stage of its evolution, (3) 2-D MAD has similar patterns with other nonpotential parameters, demonstrating that it can be utilized as an useful parameter of flare producing active region, and (4) the linear force-free coefficient could be a evolutionary indicator with a merit as a global nonpotential parameter.

In the present work we introduce a new flare activity indicator, MAD and examine its characteristics by analyzing a set of successive three days' observations of a typical active region, AR2372. The computed MAD is compared with conventional activity indicator such as separator. It is found that. (1) MAD traces very well the separator, (2) it. singles out. local discontinuity of magnetic field lines and (3) it. is a good measure of describing the evolutionary status of active region.

The test was carried out at a test field at the Wanju Eco-Farming Complex from 2009 to 2010 to figure out the proper application of fertilizers when growing rice at the EcoFarming Complex. The result showed that when compared to the basal application of compost as fertilizer, applying supplementary compost after natural re-seeding of chinese milk vetch (CM씨 helped balance soìl nutrìtìon and maìntained rice yields.

The development of humanized culture system of human embryonic stem cells (hESCs) hold promise for therapeutic applications. However, conventional culture system contain animal-derived components such as fetal bovine serum and mouse embryonic fibroblasts that bear a risk of transmitting non-human pathogens and incorporation of non-human immunogenic molecules to hESCs. In this study, we developed an efficient xeno-free hESCs culture system using humanized materials, the CELLstartTM, human foreskin feeder and xeno-free medium containing knockOutTM SR XenoFree (XF-medium) without animal-derived material. The hESCs were gradually adapted to the XF-medium; 25:75, 50:50, 75:25 and 100:0. Two karyotypically normal hESC lines, SNUhES4 and H1, were used for the experiments of xeno-free culture condition. The attachment rates at xeno-free culture system were 52.6±12.4%, 67.0±16.6%, 59.0±13.9%, 28.3±2.9% in SNUhES4, 79.3±5.4%, 53.8±20.9%, 69.4 ±6.4%, 59.8±12.6% in H1 and the spontaneous differentiation rates were 42.2±12.7%, 31.4±2.9%, 40.8±14.5%, 55.2±35.5% in SNUhES4, 35.6±8.5%, 36.4±13.5%, 48.4±7.8%, 80.1±6.0% in H1 in the first four passage. Although the attachment rates were low and the spontaneous differentiation rates were high compared to that of conventional system in the early passages using this humanized culture condition, hESCs in this culture condition were found to maintain hESC characterizations; morphology, expression of cell surface markers and stable karyotype. Our results indicate that simplified compositions of humanized culture system can be applicable to the further optimization for a xeno-free culture of hESCs without the loss of pluripotency and contamination from xenogenic sources.