In other to elucidate the neuroprotective effects of Haeahwan on oxidant-induced neurotoxicity in cultured rat spinal motor neurons, the colorimetric assay such as MTT, NR, LDH, Lipid peroxidation was also carried out after cultured spinal motor neurons from neonatal rat were treated with the medium containing various concentrations of Methylmercuric chloride(MMC) for 8 hours. In addition, the protective effect of Haeahwan water extract extracts on the neurotoxicity induced by oxygen radicals was examined in these cultures. The results were as follows : 1.Survival rate of cultured spinal motor neurons were remarkably decreased by MMC depending on different kinds of concentrations. 2.NR50 of MMC as 50μM. 3.Haeahwan water extract inhibited significantly the increase in LDH activities induced by MMC.
Weigi(衛氣) is defensive energy which moves outside the conduits, permeating the surface of the body and warding off exogenous pathogens ; superficial resistance of the body. Yinggi(營氣) is constructive energy which moves though the conduits and nourishes all the organs This volume commets on creation and gathering of Yinggi(營氣)-Weigi(衛氣). Therefore subjicts can be chosen Yong Wi Sang Whei(營衛生會). This volume contents three chapters ChapterⅠdeals that yingi(營氣) is created from zhuogi(終期) ; dense part of food essence or waste gas, e.g., the air expirde or flactus discharged; and wei is created from qinggi(淸氣); ⑴ fresh air usually referred to the air inspired in the lung ⑵ food energy or nutrient esp. the clarified thin part of food essence ⑶ clear up the evil heat from the Qi system: a method in treating febrile diseases. Chapter Ⅱ deals with the difference between a youth and adult. Chapter Ⅲ deals with the relation between yinwei(영위) and Triple Burners.
Yun gi doctrine is on five elements motion and six kinds of natural factors. Nature moves in a cycle. This phenomen on occurs periodically. Therefore everthing rotates including climate and temper. This circulation maintains the balance of nature. Yin and Yang is the two fundamental principles or forces in the universe, ever opposing and complementing each other-an ancient philosophical concept used in traditional Oriental medicine to refer to various antitheses in anatomy, physiology, pathology, diagnosis and tertment, e,g., feminine, interior, cold and hypofunction being Yin while masculine, exterior, heat and hyperfunction are Yang. Wuxing is the five evolutive phases of the five elements. That yin-yang, wuxing express the balance of nature well. This volume contents five chapters. ChapterⅠ deals with the five circuit phases and six atmospheric influences not match yin-yang. ChapterⅡ deals that Wuyun gets possession through the sky. ChapterⅢ deals with the movement's change of sitian, zaitian. ChapterⅣ deals with Liupi's movement and its effect upon human body. ChapterⅤ deals with the rule of yungi's influence.