Bioaerosols are airborne particles of biological origins including viruses, bacteria, fungi, and all varieties of living materials. In suitable hosts, bioaerosols are capable of causing acute or chornic diseases that may be infectious, allergenic, or toxigenic. Bioaerosols from outdoor air accumulate on filters of heating, ventilating and air conditioning (HVAC) system in large quantities and are able to multiply there under certain conditions. In this study, silver was coated on activated carbon fiber (ACF) filters by an electroless plating method, and their efficacy for bioaerosols removal was tested. Physical filtration and biological antimicrobial test were performed. SEM and XRD analyses were used to characterize the morphology and components of ACF filters. Electroless silver-plated ACF filters showed antimicrobial efficiency, whereas pure ACF filters did not. It was found that electroless silver plating did not influence the physical filtration efficiency of ACF filters.