본 연구의 목적은 능성어와 자바리의 정자동결을 위한 간편한 실험법개발이다. 희석제와 동해 방지제가 정자동결에 미치는 효과를 파악하고자 운동성성과 생존율을 조사하였다. 동결실험에 서 300 mM glucose와 15% dimethylsulfoxide (DMSO)를 희석제와 동해방지제로 각각 사용하였 다. 동결실험결과, 능성어 정자는 동결 5개월 후 60% 이상의 운동성을 보였고, 자바리 정자는 동결 5개월후 90% 이상의 운동성을 보였다.

A three-staged cascade virtual impactor was designed, fabricated, and used as aerosol classifier in a dust sensor module. The dust sensor module consisted of the impactor, three commercial dust sensors, and four pumps. For the design of the impactor, three cut-off diameters of 2.5 ㎛, 2.0 ㎛, and 1.5 ㎛ were selected. Then three nozzle widths were determined from computational fluid dynamics (CFD) simulation with the three designed cut-off diameters. </br>Laboratory generated PM2.5 aerosols classified into each of three sizes, via the fabricated impactor, entered a dust sensor from which voltage signals were detected due to particle scattering by a laser diode in the sensor. The voltage signal data from the three sensors were converted to number concentrations of the dust particles utilizing correlation equations obtained from separately performed experiments. The number concentrations were in agreement with those obtained by aerodynamic particle sizer (APS).