Purpose: This study aimed to explore user experience with UV-C disinfection devices in clinical settings to obtain critical information for the development of domestic devices to meet the increasing demand for efficient environmental disinfection in healthcare settings, particularly in terms of effective multidrug-resistant organism control. Methods: A qualitative approach was employed involving 21 participants (infection control nurses, staff nurses, and device managers). Data were collected through five sessions of focus group interviews, and conventional content analysis was undertaken. Results: Four categories and 13 subcategories were identified: (i) Introduction and usage status of UV-C disinfection devices, with ‘Surge in demand for rapid response to infectious diseases’ and ‘Diversified application and management of UV-C disinfection devices based on needs’ subcategories; (ii) Advantages of using UV-C disinfection devices, with ‘Significantly reduced disinfection time,’ ‘Easy and simple operation,’ and ‘Providing a sense of reassurance from a visible confirmation of disinfection’ subcategories; (iii) Limitations of current UV-C disinfection devices, including ‘Ambiguous disinfection range and presence of disinfection blind spots,’ ‘Lack of standards for disinfection efficacy verification and management,’ ‘Safety concerns regarding ultraviolet radiation exposure,’ and ‘Issues related to maintenance of UV-C disinfection devices’ subcategories; and (iv) Expectations for domestic UV-C disinfection devices, including ‘Minimization of disinfection blind spots,’ ‘Variety in device sizes,’ ‘Auxiliary devices for enhancing usage efficiency,’ and ‘Clear protocols for device usage and maintenance. Subcategories.’ Conclusion: UV-C disinfection devices offer valuable benefits for infection control; however, improvements are needed to enhance their efficacy and usability. Practical recommendations include developing standardized safety protocols, enhancing UV-C coverage, and improving device mobility and maintenance to meet diverse healthcare needs. Such advancements in UV-C technology can significantly support effective infection control and operational efficiency in healthcare settings.