This study is about a kind of organic waste, food waste, and was conducted by using an alternative medium for the cultivation of oyster mushrooms to examine applicability. The study investigated the cultivation characteristics for major cultivated varieties of oyster mushrooms and the growth characteristics within food waste mediums. In addition, NaCl desalinization of food waste is being pointed out as a problem when using the medium, therefore, the effects of the medium's composition were examined by using calcium carbonate. Calcium carbonate is used for desalinization of NaCl. This study investigated the use of a total of 6 cultivars of the oyster mushroom, the Suhan (PL.1), Chiak No. 5 (PL.2), Chiak No. 7 (PL.3), Samgu No. 5 (PL.4), Samgu No. 8 (PL.5), Jangan No. 5(PL.6). In terms of medium, the optimum growth of the mycelium of the oyster mushroom was observed in the PDA medium. The optimal culture conditions was pH 6.0 in Samgu No. 8 (PL. 5) and pH 5.5-6.6 in another cultivar. In terms of food waste, the mycelium’s growth were investigated and found insufficient in the bulk of cultivars. but Chiak No. 7 (PL.3) was superior to that of other cultivars relative to the mycelium’s growth. (Mycelium’s growth: Chiak No. 7 (PL.3) = Jangan No. 5 (PL.6) > Samgu No. 8 (PL.5) > Samgu No. 5 (PL.4) > Suhan (PL.1) > Chiak No. 5 (PL.2)). In the test of desalinization using Calcium carbonate, the early salt concentration was 4.3%. When treating the calcium carbonate (1 ~ 10%) in the food waste, a change of salt concentration less than 5% of the calcium carbonate could be observed. Also, when treating the calcium carbonate (1 ~ 10%, w/w) in the food waste medium, a great change of EC could not be observed either. Therefore, the addition of calcium carbonate to food waste does not affect the growth of the mushroom, thus we had to speculate about the possibility of using food waste in the cultivation of oyster mushrooms.