눈꽃동충하초 분생자경속을 형성하는데 액체배양을 이용한 접종원의 배지는 PDB가 가장 우수하였으며, 분생자경속 형성에 유리한 배양온도는 20℃로 나타났다. 조명은 500lx.에서 가장 양호한 성장을 하였으며 광조사 시간은 18hr이 가장 유리한 분생자경형성 조건을 나타내었다. 알맞은 배지 조건은 번데기와 현미를 50 : 50 의 비율에 증류수를 현미에 1 : 1비율로 첨가한 혼합배지에서 높게 나타났다. 번데기배지는 원형상태와 분쇄상태 중에서는 분쇄된 상태에서 번데기배지의 공극률이 높아 균사의 침입이 용이하여 분생자경속 회수율과 균사의 배지내에 형성이 원형상태의 번데기보다 더욱 높게 나타났으며, 균사의 생장도 역시 분쇄한 번데기에서 더욱 빠르고 고르게 나타났다. 종균 접종량은 15㎖의 종균을 접종하면 분생자경속이 잘 형성되었다. 눈꽃동충하초 재배에 가장 안정하게 재배하려면 분쇄한 번데기에 현미와 혼합한 배지에 종균 15 ㎖를 접종하여 24 에서 발이를 시킨 후 20℃의 배양실에서 재배하는 것이 가장 좋은 것으로 나타났다.

본시험은 한국에서 채집되어 강원대학교 동충하초은행(EFCC)에서 보관 중인 눈꽃동충하초의 분포와 배양적 특성을 조사하였다. 눈꽃동충하초는 한국의 산이나 계곡에서 어렵지 않게 채집할 수 있으며 골고루 분포한다. 채집시기는 7∼9월로 나타났으며 9월말에 가장 많이 채집할 수 있으며 채집된 눈꽃동충하초의 종은 눈꽃동충하초(P. tenuipes)가 가장 높게 채집되었다. 눈꽃동충하초의 균사배양 최적배지는 PDA배지이며 SDAY와 HMA배지에서도 생장은 가능하며 균사생장에 가장 적합한 온도는 25℃이며 pH는 6.0∼7.0으로 나타났다. 적합한 영양원으로는 탄소원은 dextrose이었으며, 질소원은 tryptone가 가장 우수하였고, 무기염으로는 KH2PO4에서 가장 고른 성장을 보였다. C/N에서는 20 : 1의 비율이 가장 적합한 것으로 조사되었다.

This study was performed to investigate the feasibility of ion-exchange resins as soil amendment. To select resins, weinvestigate four kinds of ion exchange resins, which ions have the most adsorption efficiency of ions (N+, PO4−, K+). Inthe Cation (H+) vs Anion (OH−)=1:2 (w:w), it showed that the highest N, P, K adsorption efficiency was about 80%and reached pH 6~7 & EC 1~2dS/m after 20min. The ability to maintain ion adsorption, it was investigated that thegentle curve of the residual ion ratio of nitrate nitrogen, available phosphorus, exchangeable potassium was maintained.On the other hand, the control (Slow release fertilizer) was significantly reduced under artificial rainfall conditions. Inaddition, the results of three consecutive crop test were showed that the growth of crops decreased 41.8% than the earlygrowth in the control group, whereas decreased 8.3% in the treatment group (ion exchange resins). According to the resultsof soil analysis, it was reduced that the fertilizer ingredients (N 94%, P 74.5%, K 98.6%) in the control and reducedfertilizer ingredients (N 64%, P 60.3%, K 58.4%) in the treatment. Overall, it was estimated that the Ion-exchange resinscould be used as a soil amendment.

This study is about making artificial soil for wall greening using Spent Mushroom substrates (SMS) & Superabsorbent polymer (SAP). and developing new material for wall greening whilst monitoring the physico-chemistry & germination ability of the plant, plant growth and developmental condition of the artificial soil. It was found that the larger the particle size of the superabsorbent polymer the more absorption increased. This showed the plant’s germination rate in soil when Spent Mushroom substrates & Superabsorbent polymer (SAP) were mixed. In the Bermuda grass, the Artificial soil (SMS & SAP 0.5%) showed a 100% germination rate over a period exceeding 7 days compared to Peat-moss that showed a 94% germination rate. In the Kentucky blue grass, however, there was no difference between the Peat-moss and Artificial soil. When general perlite soil was compared with artificial soil in the chicory seed, the Artificial soil showed better results than the general perlite soil in length, leaf volume and fresh-weight. In the plant length, it showed an increase of over 105% (SAP 0.5% or more), 187% (SAP 1% or more). in the leaf volume, an increase of over 123% (SAP 0.5% or more), 145% (SAP 1% or more), in the fresh-weight an increase of over 130% (SAP 0.5% or more), 285% (SAP 1% or more). The artificial soil (SMS & SAP 1%) showed an excellent result in the plant growth and development. Thus, it was found that artificial soil using the SMS & SAP were the only suitable soil materials for wall greening hence suggesting that it could be used for the greening of a blighted area or desert and a variety of agriculture.

This study is about a kind of organic waste, food waste, and was conducted by using an alternative medium for the cultivation of oyster mushrooms to examine applicability. The study investigated the cultivation characteristics for major cultivated varieties of oyster mushrooms and the growth characteristics within food waste mediums. In addition, NaCl desalinization of food waste is being pointed out as a problem when using the medium, therefore, the effects of the medium's composition were examined by using calcium carbonate. Calcium carbonate is used for desalinization of NaCl. This study investigated the use of a total of 6 cultivars of the oyster mushroom, the Suhan (PL.1), Chiak No. 5 (PL.2), Chiak No. 7 (PL.3), Samgu No. 5 (PL.4), Samgu No. 8 (PL.5), Jangan No. 5(PL.6). In terms of medium, the optimum growth of the mycelium of the oyster mushroom was observed in the PDA medium. The optimal culture conditions was pH 6.0 in Samgu No. 8 (PL. 5) and pH 5.5-6.6 in another cultivar. In terms of food waste, the mycelium’s growth were investigated and found insufficient in the bulk of cultivars. but Chiak No. 7 (PL.3) was superior to that of other cultivars relative to the mycelium’s growth. (Mycelium’s growth: Chiak No. 7 (PL.3) = Jangan No. 5 (PL.6) > Samgu No. 8 (PL.5) > Samgu No. 5 (PL.4) > Suhan (PL.1) > Chiak No. 5 (PL.2)). In the test of desalinization using Calcium carbonate, the early salt concentration was 4.3%. When treating the calcium carbonate (1 ~ 10%) in the food waste, a change of salt concentration less than 5% of the calcium carbonate could be observed. Also, when treating the calcium carbonate (1 ~ 10%, w/w) in the food waste medium, a great change of EC could not be observed either. Therefore, the addition of calcium carbonate to food waste does not affect the growth of the mushroom, thus we had to speculate about the possibility of using food waste in the cultivation of oyster mushrooms.