The content of luteolin in the different parts of Lithospermum erythrorhizon was determined by high performance liquid chromatography (HPLC). Luteolin was quantified by a reverse-phase column with gradient solvent program (water : ACN = 90 : 10 to 77 : 23 for 60 min). UV detection was conducted at 330 nm. The content of luteolin was measured in the seeds (0.28 mg/g), stems (0.30 mg/g), and roots (0.16 mg/g) of L. erythrorhizon. The presence of luteolin in Lithospermum species is important in agricultural crop production for increasing the amounts of clinically available medicine and health supplements.
The chemical composition of essential oil from the perennial herbaceous plants (Houttuynia cordata, Filipendula glaberrima, Peucedanum japonicum, and Ainsliaea acerifolia) was determined by GC/MS spectrometric analysis with the aid of NBS, Wiley Library and RI indice searches. The major constituents identified were α-phellandrene (18.97%), γ-terpinene (12.32%), decanal (8.72%), 1-decanol (10.92%), decanoic acid (12.12%), and 2-undecanone (12.32%) from H. cordata, farnesol (2.83%), l-α-terpineol (2.72%), benzenmethanol (2.03%), (Z)-3-hexen-1-ol (4.32%), and T-muurolol (2.07%) from F. glaberrima, α-phellandrene (14.25%), endobornyl acetate (3.84%), heptanal (47.52%), octanal (2.65%), (E,E)-2,4-decadienal (2.75%), and octanoic acid (4.52%) from P. japonicum, and geyrene (9.74%), β-cubebene (11.15%), berkheyaradulen (22.32%), β-elemene (6.21%), (-)-A-selinene (4.85%), benzaldehyde (4.52%), and benzenacetaldehyde (3.40%) from A. acerifolia.
한국특산 섬오갈피나무 및 지리산오갈피나무의 잎과 줄기에서 추출한 주요 정유성분으로, 섬오갈피나무의 경우에는 δ-3-carene (31.34%), l-limonene (17.01%), β-elemene (4.53%), trans-p-menth-2-ene-1,8-diol (3.13%), 1,8-cineole (4.73%), 1-dodecen-3-yne (2.64%), (Z)-3,7-dimethyl-1,3,6-octatriene (3.21%), 지리산오갈피나무의 경우에는 delta-3-carene (14.78%), limonene (7.24%), t-ocimene (7.22%), α-terpinolene (8.76%), gamma-elemene (4.32%), β-selinene (7.72%), veridifloral (3.25%), dodecane (2.44%) 등의 성분함량이 높음을 알 수 있었다. 특히 δ-3-carene는 섬오갈피나무 및 지리산오갈피나무에서 가장 높은 함량을 나타내었다.