The purpose of this study was to investigate emotional images and preference of knitwear by tone on tone combination. The subjects were 357 university students in Daejeon and Chungnam province, and the measuring instruments were 6 stimuli manipulated by color and tone combination type of background and pattern in the tone and tone combination, and self-administrated questionnaires consisted of emotional images items, preference items, and subjects' demographics attributions. The data were analyzed by Cronbach's α, factor analysis, t-test, MANOVA and Duncan's multiple range test, using SPSS program. The results were as follows. First, four factors (attractiveness, conspicuity, mildness, and activity) are emerged on emotional images of knitwear. Second, color had main effects on emotional images and preference. Gray color was perceived as most attractive image and more preferred than others. Third, tone combination type had some effects on emotional images. Vivid tone background/light tone pattern was perceived more attractive image but less conspicuous and mild than light tone background/vivid tone pattern. Forth, subjects' gender had an effects on conspicuous image. Male was perceived more conspicuous image on knitwear stimuli than female. Fifth, color and subjects' gender had interaction effects on attractiveness image and preference. Male perceived that blue is more attractive and preferred than female.