Immunohistochemical localization of vimentin, glial fibrillary acidic protein (GFAP), myelin basic protein (MBP), neuron-specific enolase (NSE) were performed to confirm the canine schwannoma diagnosed histologically. Biopsy samples from skin masses occurred on a 6 year old female dog and a 13 year old female dog were used for immunohistochemical analysis. Histologically, the tumor tissues showed the characteristics of schwannoma mainly composed of predominantly fusiform or spindle cells. The cells are arranged in small interwoven bundles with intervening fibrous and mucinous stroma that imitate a herringbone pattern. The cell nuclei have an elongated, wavy, buckled appearnce and closely bunched together. In high magnification, mitotic figure, nuclear hyperchromatism, pleomorphism and poorly defined cytoplasms were noted. the vimentin was highly expressed in the cytoplasm of all neoplastic cells. Positive reactions against NSE and GFAP, widely used to suggest a neural origin of cell types, are observed in cytoplasms of tumor cells. These results can be used as a reference data for definitive diagnosis of canine schwannoma.