Concentrations of major minerals(Ca, P, Na, K, Mg) and trace elements(Fe, Zn) were measured in human milk samples collected from 19 selected, healthy lactating women at 2∼5 days, and at 4, 8 and 12 weeks postpartum. Decreases in the contents of major minerals and trace elements were found with the increase of time postpartum. Mean Ca contents of 2∼5 days, 4 week, 8 week and 12 week in human milk were 243.02±50.90㎍/㎖, 295.0±65.70㎍/㎖, 295.0±41.50㎍/㎖, 349.90±83.50㎍/㎖, respectively. And mean P contents of 2∼5 days, 4 week, 8 week and 12 week in human milk were 134.70± 49.40㎍/㎖, 134.90±19.70㎍/㎖, 117.60±18.90㎍/㎖, 130.60±28.20㎍/㎖, respectively. Ca/P of them were 1.81, 2.19, 2.20, 2.68, respectively. Mean Mg contents of them were 22.30±5.60㎍/㎖, 25.70±3.70㎍/㎖, 25.10±3.60㎍/㎖, 32.10±4.50㎍/㎖, respectively. And mean Na contents of them were 171.20±90.50㎍/㎖, 158.70±47.00㎍/㎖, 104.50±24.70㎍/㎖, 115.90±12.50㎍/㎖, respectively. Mean K contents of them were 506.10±156.10㎍/㎖, 520.90±55. 00㎍/㎖, 370.10±29.00㎍/㎖, 468.40±75.70㎍/㎖, respectively. Na/K of them were 0.34, 0.30, 0.28, 0.25, respectively. Mean Fe contents of them were 2.80±1.80㎍/㎖, 2.70±0.40 ㎍/㎖, 2.60±0.40㎍/㎖, 2.10±0.10㎍/㎖, respectively. And mean Zn contents of them were 3.70±1.60㎍/㎖, 2.78±9.70㎍/㎖, 2.70±0.70㎍/㎖, 2.30±0.20㎍/㎖, respectively. These determinations will provide the basic information on the variability of major minerals and trace elements as lactation proceeds and on the composition of components between term and preterm milk.