Background : We are Corporation of Reborn International that studies herbal medicine (Hydroponics Peanut sprout) and natural substance hydroponics. Our work is hydroponically grown peanut sprouts. This introduce, mouse allergic efficacy test and peanut tissue culture were performed. Methods and Results : The peanut sprouts have been widely used for long time of years in many Asian countries. P. sprouts germinated from peanuts, which contain 4 times higher resveratrol compounds than peanuts. The resveratrol have bioactivity effects as anti-inflammatory and anti-cancer. Especially, Our virgin premium peanut sprouts oil (cold pressure; low temperature extraction), natural substance oil produced mainly in peanut sprouts are used for valuable foods and cosmetic that supply essential fatty acids for humans as well as industrial raw functional materials production. In this work, large scale-up production of peanut sprout using hydroponics. Using extracts of peanut sprouts, various efficacy screening, animal test (in vivo, in vitro), and analyzes were also performed. Also, the culturing the peanut callus for increasing the contents of a specific useful ingredient were cultured in modified Murashige-Skoog/MS (sucrose 2%, agar 0.8%, pH 5.7) solid and liquid medium. It was examined that 0, 1, 3, 5, 10 ㎎/ℓ of NAA and 0, 1, 3, 5, 10 ㎎/ℓ of IBA were the callus and shoot induction. It's, developed sample model of food products of various formulations or form of including from peanut sprout extracts. Conclusion : The essential amino acids (composition unsaturated fatty acid) and resveratrol were abound to the extracts. Also, The blood circulation and allergy improvement effect was excellent. And toxicity were not detected in efficacy. Tissue culture screening work, It’s examined that〈 1.0 ㎎/ℓ of NAA and〈 3.0 ㎎/ℓ of IBA were the best composition. However, depending on the purpose of securing the functional material, a more detailed investigation is needed. So, future work is induce roots callus and shoot. The shown results can be applied as sources for functional food, cosmetic, pharmaceuticals, and material.