In this study, we investigated the effect of bisphosphonate on the osteoblastic differentiation of human dental stem cells (hDPSCs). In the first experiment, we evaluated the effect of bisphosphonate on the differentiation of hDPSCs into osteoblasts by alkaline phosphatase staining after culturing hDPSCs. As a result, on day 13, the osteogenic differentiation of hDPSC was suppressed at 5 μM in clodronate and 2 μM in zolendronate. In NBP, osteogenic differentiation is more suppressed. In second experiment, cytotoxicity and proliferation test, the cell proliferation (examined by MTT assay) was more suppressed as the concentrations of zolendronate were larger than those of alendronate and clodronate. Western blotting, a third experiment, was found that AKT phosphorylation was inhibited in cell signaling proteins involved in cell proliferation inhibition and death by bisphosphonate concentration. In human dental stem cells, bisphosphonates inhibit osteoblast differentiation, and this phenomenon is clearly observed in NBPs (zolendronate), and it has been found that it is related to AKT phosphorylation of cell signaling proteins.

Recently, serious construction site accidents are increasing. At present, the accident rate of the whole industry is 0.5 ('15), while the accident rate of the construction industry is 0.75 ('15), which is 50% higher than the accident rate of the whole industry. In order to reduce the accident rate of the construction industry to the level of the accident rate of the whole industry, there are various methods such as compliance with specifications, drawing and specification, thorough supervision of management, most importantly, it is necessary to recognize that the safety management of construction work is not the work of the builder only, and that it is the task of all participants of the construction work (contractor, designer, builder, construction management). In addition, it should be based on the institutional part to support that. As a part of this, design for safety was introduced to prevent disasters occurrence in construction work. Design for safety was introduced to prevent disasters occurrence in construction work. In this study, we intend to elaborate domestic and overseas institutions and revitalization plans.

Recently, serious construction site accidents are increasing. At present, the accident rate of the whole industry is 0.5 ('15), while the accident rate of the construction industry is 0.75 ('15), which is 50% higher than the accident rate of the whole industry. In order to reduce the accident rate of the construction industry to the level of the accident rate of the whole industry, there are various methods such as compliance with specifications, drawing and specification, thorough supervision of management, most importantly, it is necessary to recognize that the safety management of construction work is not the work of the builder only, and that it is the task of all participants of the construction work (contractor, designer, builder, construction management). In addition, it should be based on the institutional part to support that. As a part of this, design for safety was introduced to prevent disasters occurrence in construction work. Design for safety was introduced to prevent disasters occurrence in construction work. In this study, we intend to elaborate domestic and overseas institutions and revitalization plans.