산업과 기술의 발전으로 인해 수계로의 화학물질 배출이 증가하고, 이로 인해 환경오염과 인체 건강에 부정적인 영향을 미치 는 위험이 더욱 증가하였다. 따라서, 수질을 종합적으로 평가할 수 있는 생태독성평가의 중요성이 강조되고 있다. 본 연구에서는 렌즈프 리 그림자 이미징 기술을 활용한 Cellytics 플랫폼을 소개하며, 화학물질에 의한 로티퍼(Brachionus plicatilis)와 미세조류(Dunaliella tertiolecta) 의 생물학적 변화를 신속하게 측정하고 독성을 분석하는 기법을 제안한다. 이를 위해 로티퍼와 미세조류를 톨루엔에 각각 1분과 5분 동 안 노출한 뒤, Cellytics를 이용하여 로티퍼의 이동성과 미세조류의 형태 변화를 측정하여 독성을 평가하였다. 로티퍼의 이동성과 미세조류 의 형태변화는 모두 110.4 mg/L의 농도에서 대조군과 유의미한 차이를 나타내며(p<0.05), 이는 로티퍼의 생존율로 분석한 톨루엔의 LC50(552 mg/L)보다 낮은 농도였다. 본 연구에 따르면, 로티퍼와 미세조류를 전통적인 방식으로 최소 수 일 간 배양하여 얻을 수 있는 생 태독성평가 결과를 매우 짧은 시간(5분 이내)에 분석하고, 두 생물의 독성평가 결과를 신속하게 제공하여 현장에서 활용 가능한 신뢰성 높은 정보를 제공할 수 있음을 보여준다. 이는 독성평가를 이용하는 다양한 연구의 활용에 기여할 수 있으며, 환경보호 및 인체 건강 관 련 정책 수립에 도움이 될 것으로 기대된다.

Background: Ameloblastoma are benign but locally invasive neoplasms that represent 10% of all odontogenic tumors. Despite its benign characteristics, ameloblastoma has a high recurrence rate after treatment with a recurrence rate of 55-90%. It is important to identify the risk factors of recurrence to improve patient’s quality of life in oral and maxillofacial surgery. Methods: Patients who underwent surgery at the Department of Oral and Maxillofacial Surgery at Pusan National University Dental Hospital for 5 years from 2017-2021 and were diagnosed with ameloblastoma as a result of postoperative histological examination were included. The patients were divided into two groups, recurrent and non-recurrent, and comparative analysis was performed according to various factors. Results: First, when the lesion was involved with the inferior alveolar nerve (IAN), recurrence was more likely than when it was not. Next, recurrence occurs more often when cortical bone perforation is observed than when it is not. In particular, when resorption is shown on the lingual cortical bone, a remarkable tendency of recurrence is shown. Moreover, in radiographic characteristics, the multicystic type showed a higher recurrence tendency than the unicystic type. Conclusion: When the lesion is multicystic, perforating the cortical bone, infiltrating the adjacent soft tissue, or involving the IAN, a high recurrence rate is shown. The results of this retrospective analysis of the recurrence trend of ameloblastoma over a 5 years period are to contribute significantly to insight and reduction of recurrence rates in treatment for polymorphic lesion in oral and maxillofacial area.

The myxoma(odontogenic myxoma, myxofibroma) of the jaws is a benign, usually slow-growing infiltrative tumor of connectice tissue. This article describes a case history of the treatment of recurred odontogenic myxofibroma in the maxilla and further discusses the appropriate management of such cases with reference to the literature review. A 55-year-old male patient presented with a gingival swelling in the right side of maxilla. He had history of second time recurred disease. Microscopically, a high cellularity fibrous tumor with fibroblastic cells was observed. Tumor cells were composed of stellate and spindle shaped fibroblasts. Islands of odontogenic epithelium were seen in the specimen. The final diagnosis was made with odontogenic myxofibroma in consideration of twice-time recurrences and histological characteristics. Because of the high likelihood of recurrence, an accurate differential diagnosis of pathology at the time of initial visit is very important in determining treatment options, and immunohistochemical tests may be helpful. Also close following up is required.(155 words)

The purpose of this study was to identify the oral bacterial species in sequestra from patients with bisphosphonate-related osteonecrosis of the jaw (BRONJ). Fifteen patients with BRONJ (2 males and 13 females) were evaluated. Clinical features, radiographic findings, and bisphosphonate intake history were investigated. All patients were treated with surgical methods (curettage or sequestrectomy). Infected bone samples were collected from the affected BRONJ site. Ten bacterial species were selected for polymerase chain reaction (PCR) detection. Two to nine bacterial species were detected by PCR. Gram-negative species were predominant and all identified bacteria were anaerobes. Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola were detected at high levels. These are major pathogenic species in periodontal disease. Orthopantomographic radiographs showed generalized alveolar bone loss in most patients. These radiographic findings may provide evidence of chronic periodontitis as a pre-existing inflammatory disease. Most patients had experienced a predisposing dental procedure, such as tooth extraction. Sequestra (necrotic bone) infected with oral bacterial species may be an important risk factor for BRONJ. As such, prevention and management of BRONJ may rely on effective control of bacteria in the oral cavity.

우리나라의 조선해양플랜트 산업은 2011~2015년 기간에 우리나라 품목별 수출기여도에서 1~4위를 차지할 정도로 대한민국 경제성장을 견인하는 중요한 산업이다. 이 연구는 과거 건조에만 치중했던 우리나라 해양플랜트 시추설비의 산업구조에서 통합시운전의 서비스 공급이 가능한 비즈니스 모델을 검토함으로써 시추설비 시장에서 우리나라 조선소의 국가경쟁력을 향상시킬 수 있는 방안을 제안 하고자 하였다. 2014년에 시도된 주작-1 폐공을 활용한 시운전 서비스 모델과 이의 한계 사항을 극복하고 사업을 활성화하기 위한 새로운 제안 비즈니스모델인 수심 150미터급 시운전 전용 모델의 경제성을 검토하였다. 경제성 평가 결과, 수심 150미터급 모델이 경제적으로 더 효과적인 것으로 나타났다. 그리고 통합시추설비의 시운전이 연간 2회에서 3,4,5회로 늘어남에 따라 NPV와 IRR 및 B/C 비율이 모두 증가 하였으며, 1회당 사용료는 감소하는 것으로 나타났다. 따라서 해양시추설비의 통합시운전 사업은 우리나라에서 건조되어 인도되는 설비를 대상으로 시추 시운전을 적극적으로 유치하는 노력이 필요하다.

In this study, we investigated the effect of bisphosphonate on the osteoblastic differentiation of human dental stem cells (hDPSCs). In the first experiment, we evaluated the effect of bisphosphonate on the differentiation of hDPSCs into osteoblasts by alkaline phosphatase staining after culturing hDPSCs. As a result, on day 13, the osteogenic differentiation of hDPSC was suppressed at 5 μM in clodronate and 2 μM in zolendronate. In NBP, osteogenic differentiation is more suppressed. In second experiment, cytotoxicity and proliferation test, the cell proliferation (examined by MTT assay) was more suppressed as the concentrations of zolendronate were larger than those of alendronate and clodronate. Western blotting, a third experiment, was found that AKT phosphorylation was inhibited in cell signaling proteins involved in cell proliferation inhibition and death by bisphosphonate concentration. In human dental stem cells, bisphosphonates inhibit osteoblast differentiation, and this phenomenon is clearly observed in NBPs (zolendronate), and it has been found that it is related to AKT phosphorylation of cell signaling proteins.