This study was conducted to examine effects of plant growth regulators and light resources on the formation of multiple shoot and plant regeneration of Hovenia dulcis var. koreana Nakai. Stem and shoot tip were cultured on MS medium or WPM supplemented with various plant growth regulators. At the single treatment, the highest shoot formation was obtained when stem explants were cultured on WPM supplemented with kinetin 1.0mg·L-1. MS medium containing NAA 0.1 and TDZ 0.1mg·L-1 gave the best results for shoot induction rate and shoot growth in combination treatments. Of the BAP and kinetin tested, BAP 0.5mg·L-1 on WPM was found to be more effective for shoot growth from shoot tip. Under white fluorescent light treatment, shoot growth was much higher than blue, red LED treatments. Root induction from in vitro growth of plantlet was the best on WPM supplemented with 1.0mg·L-1 IBA. The results suggest that selection of plant growth regulators and light resources could be important factor to achieve an efficient in vitro growth.