The effects of conventional rolling (CR) and differential speed rolling (DSR) on the microstructure and mechanical properties of a copper alloy sheet were investigated in detail. A copper alloy with thickness of 3 mm was rolled to a 50% reduction at ambient temperature without lubrication with a differential speed ratio of 2:1; sample was then annealed for 0.5h at various temperatures from 100 to 800˚C. Conventional rolling, in which the rolling speed of the upper and lower rolls is identical, was performed under the same rolling conditions. The shear strain introduced by the CR showed positive values at positions on the upper roll side and negative values at positions on the lower roll side. However, the shear strain showed a zero or positive value at all positions for the samples rolled by the DSR. The microstrucure and mechanical properties of the as-rolled copper alloy did not show very significant differences between the CR and DSR for the microstructure and mechanical properties. However, those properties showed very significant differences in the case of the annealed samples. The effects of rolling method on the microstructure and mechanical properties of the as-rolled and subsequently annealed materials are discussed in terms of the shear strain.
저온 스트레스에 대한 내성을 지닌 신품종 톨 페스큐를 개발할 목적으로 CaMV35S 프로모터 하류에 NDP kinase 2 유전자가 항상적으로 발현하도록 제작한 벡터를 Agrobacterium법을 이용하여 톨 페스큐에 도입하였다. Hygromycin이 첨가된 선발배지에서 내성을 나타내며 재분화된 형질전환 식물체를 pot로 이식하여 기내순화 시킨 후, Southern blot 분석을 실시하여 본 결과, NDP kinase 2 유전자가 형질전환 식물체의 g
환경스트레스에 강한 내성을 지닌 신품종 톨페스큐를 개발할 목적으로 산화스트레스에 의해 강하게 유도되는 SWPA2 promoter 하류에 CuZnSOD와 APX 유전자가 엽록체에 동시에 발현하도록 제작한 벡터를 Agrobacterium법을 이용하여 톨 페스큐에 도입하였다. Hygromycin이 첨가된 선발배지에서 내성을 가지며 재분화된 형질전환 식물체를 pot로 이식하여 기내 순화시킨 후, Southern 분석을 실시하여 본 결과, 발현벡터의 T-DNA