In this study, effects of nutrient and inorganic carbon on single cell emergence during the cultivation of microalgae were observed using colonial green algae, Pediastrum duplex. The concentration of inorganic carbon had significant effect on single cell emergence and its growth, but nitrogen and phosphorus concentration showed minor effects. According to P. duplex cultivation experiment, single cell started to be emerged around 500~750 mg-C/L of inorganic carbon concentration and it was bloomed dramatically at the higher values. And growth of P. duplex was started to be surpressed at the single cell formation concentration. From the results, it could be said that when we operate the microalgae systems for cultivation/harvesting or wastewater treatment, in order to avoid single cell formation, inorganic carbon should be maintained to the proper level

In order to evaluate the applicability of GWLF model which can efficiently estimate non-point and point source pollutant loadings in rural watershed including urban district, the model was applied to an experimental watershed. The model was calibrated using observed data such as daily runoffs, sediment yields, T-N, and T-P. Simulated daily runoffs and sediment yields by the model using calibrated parameters were in food agreement with the observed data. There were difference between the simulated and observed nutrient loading which was considered resonable. The simulated results by the model showed that T-N, T-P and sediment yields were dependent on the amount of stream runoff discharge and land use. GWLF model is believed to applicable to estimate amount of pollutant loading of non-point source pollution for the water qualify control of agricultural watersheds.