The World Vegetable Center (WorldVeg) maintains the world’s largest public vegetable germplasm collection. The Genebank contains over 65,000 accessions and consists of more than 130 genera and 330 species. It includes around 12,000 accessions of indigenous vegetables. The WorldVeg Korea Office (WKO) has been conducting regional adaptability evaluations in the fields of the National Institute of Horticultural and Herbal Science (NIHHS), with a focus on major solanaceous vegetable crops such as tomato, chili, and bell pepper. As climate change significantly affects vegetable crop productivity, the development of high-performing cultivars, tolerant to various abiotic and biotic stresses with enriched nutrients inside, is essential to enhance agricultural sustainability and human health. In this regard, the evaluation of growth and horticultural characteristics of pepper and tomato accessions from the WorldVeg will help contribute to the generation of new and improved cultivars, to address global challenges that affect food security, sustainability, and adaptation to climate change. Here, we summarize our activities and the results of the regional adaptability evaluation using the WorldVeg accessions of pepper and tomato germplasms.