Anthocyanins are the major pigments contributing to flower coloration. A 1584 bp 5' upstream sequence of ALCHS2 gene was isolated from Acapulco lily (Lilium Oriental hybrid cv. Acapulco). Computer-based analyses (GeneScan, AtPAN) predicted a CAATBOX1 and putative transcription factor-binding sites, including tissue-specific elements. When gALCHS7 promoter–gus fusion was introduced to petunia ('Dream Red'), all ten putative transgenic plants showed localized GUS activity in the anther, but five of them also showed weak GUS activity in the ovule. No distinctive signal in the leaf and petal was detected in the same stage. To clearly determine the operation of the promotor region, anther and ovule tissues of transgenic line 6 were fixed in paraffin for dark-field analysis. At 1 cm length of floral bud, a GUS signal was not observed in the anther, but weak expression was observed in the ovule. Before anthesis, GUS protein was highly expressed in the pollen, endothecium, and epidermis. Fluorometric GUS assays of individual organs taken from four transgenic plants demonstrated that all lines showed high GUS activity in the anther compared to 35S CaMV promoter (pBI1 121), except line 34. Using the truncated promoters by cis-acting elements, we found that minimal region (gALCHS7-7, 270 bp) displayed GUS expression only in the anther, though at weaker activity than in the original promoter.

아시아틱나리 ‘다프네(Daphne)’ 품종은 국립원예특작과학원에서 2009년에 육성되었다. 2000년 적색의 원예원 계통인A95-45(Avignon × Connecticut King)’ 를 모본으로 하고,황색의 아시아틱나리 ‘황금(Hyanggum)’ 품종을 부본으로 교배하였다. 2004년 교배실생 중에서 ‘A04-67’계통을 선발하였다. 2005년부터 2008년까지 조직배양에 의한 대량증식, 순화 및 양구를 거쳐 생육 및 특성검정을 수행하였다. 특성평가는 2009년에 ‘원교 C1-105호’로 수행되었고, 국립종자원에‘다프네’ 품종으로 출원 및 등록 되었다. ‘다프네’ 품종의 개화기는 6월 초순이다. 꽃은 상향으로 개화하고, 화색은 분홍색과 아이보리색의 복색(RHS, R61D+W115B)이다. 초장은77.8 cm이고, 꽃의 크기는 9.6 cm로 소륜이다. 꽃잎은 길이가 5.8 cm이고, 폭은 2.2 cm이다. 잎의 길이는 9 cm이고, 폭은 1.4 cm이다. 구근의 무게는 58.9 g이고, 구주는 17.2 cm이다. 주년재배를 위해서는 -1.5℃에 구근을 동결 저장하여정식시기를 달리하여 활용할 수 있다.

Shoot tips of chinese yam (Dioscorea opposita Thunb.) were cultured on MS medium containing 0.5 mg/L BA to produce micro-tubers in vitro. To stimulate the formation of shoots and micro-tubers, and produce large micro-tubers, the sections of micro-tubers were cultured on MS media with BA and IAA. The shoot multiplication, and the micro-tuber formation and growth were very effective on the media containing 2.0 mg/L BA and 0.5~1.0 mg/L IAA. Sucrose added to MS medium with 2.0 mg/L BA and 0.5 mg/L IAA to stimulate more micro-tuber growth. The medium added 50 g/L sucrose was very effective in the increase of plant fresh weight and micro-tuber growth. After 4 weeks' culture of micro-tuber sections on the medium with 2.0 mg/L BA, 0.5 mg/L IAA and 50 g/L sucrose, the liquid media were added into the same vessels. The micro-tuber growth was stimulated remarkably by the addition of liquid medium. The addition of 25 ml liquid medium containing 10 g/L activated charcoal, 3x MS salts and 250 g/L sucrose was the most effective in micro-tuber growth.