본 연구에서는 단기간의 UV-A 조사가 시금치(Spinacia oleracea L.)의 생장과 생리활성물질에 미치는 영향을 평가 하였다. 시금치 묘는 200μmol·m-2·s-1 PPFD, white LED, 광 주기 12시간, 온도 20°C, 상대습도 70%, 이산화탄소 농도 500μmol·mol-1의 수직농장 모듈에서 재배되었다. 파종 후 5 주된 묘는 7일 동안 20W·m-2와 40W·m-2의 두 가지 에너지 수 준에서 연속적으로 UV-A(피크파장: 385nm) 조사한 후 생육 특성, 광합성 파라미터, 이미지 형광, 총 페놀 함량, 항산화도, 그리고 총 플라보노이드 함량을 분석하였다. 결과적으로, UV-A20W 처리는 시금치의 생체중과 건물중을 증가시켰다. 하지만, UV-A 처리구와 대조구 사이의 광합성 파라미터에는 유의한 차이가 나타나지 않았다. 광계Ⅱ의 최대양자수율 (Fv/Fm)은 모든 UV-A 처리에서 7일동안 지속적으로 감소했 다. 또한, UV-A20W 처리에서 식물체당 총 페놀 함량과 항산화 도는 처리 7일째 증대되었으며, 총 플라보노이드 함량은 처리 5일째부터 유의적으로 증가하였다. 이러한 결과는 UV-A LED 보광이 수직농장과 같은 폐쇄형 식물 생산 시스템에서 재배되는 시금치의 생장과 품질을 향상시킬 수 있음을 시사 한다.

Ultra-violet (UV) light is one of abiotic stress factors and causes oxidative stress in plants, but a suitable level of UV radiation can be used to enhance the phytochemical content of plants. The accumulation of antioxidant phenolic compounds in UV-exposed plants may vary depending on the conditions of plant (species, cultivar, age, etc.) and UV (wavelength, energy, irradiation period, etc.). To date, however, little research has been conducted on how leaf thickness affects the pattern of phytochemical accumulation. In this study, we conducted an experiment to find out how the antioxidant phenolic content of kale (Brassica oleracea var. acephala) leaves with different thicknesses react to UV-A light. Kale seedlings were grown in a controlled growth chamber for four weeks under the following conditions: 20°C temperature, 60% relative humidity, 12-hour photoperiod, light source (fluorescent lamp), and photosynthetic photon flux density of 121±10 μmol m-2 s-1. The kale plants were then transferred to two chambers with different CO2 concentrations (382±3.2 and 1,027±11.7 μmol mol-1), and grown for 10 days. After then, each group of kale plants were subjected to UV-A LED (275+285 nm at peak wavelength) light of 25.4 W m-2 for 5 days. As a result, when kale plants with thickened leaves from treatment with high CO2 were exposed to UV-A, they had lower UV sensitivity than thinner leaves. The Fv/Fm (maximum quantum yield on photosystem II) in the leaves of kale exposed to UV-A in a low-concentration CO2 environment decreased abruptly and significantly immediately after UV treatment, but not in kale leaves exposed to UV-A in a high-concentration CO2 environment. The accumulation pattern of total phenolic content, antioxidant capacity and individual phenolic compounds varied according to leaf thickness. In conclusion, this experiment suggests that the UV intensity should vary based on the leaf thickness (age etc.) during UV treatment for phytochemical enhancement.

최근, 소비자들이 건강관리에 대한 관심과 중요도가 높아짐 에 따라 화학적인 농약을 사용하지 않은 유기농 천연 식품을 선호하게 되었다. 새싹 채소는 건강증진과 암, 심혈관질환 등의 질병을 예방하는 효과가 있어, 고기능성이고 안전한 식품 인 새싹 채소의 소비가 크게 증가하고 있는 추세이다. 이 연구의 목적은 여러가지 새싹 채소 7종의 생육 그리고 생리활성 물질 함량에 대한 광의 영향을 조사하는 것이다. 새싹 채소용 케일, 다채, 브로콜리, 적양배추, 알팔파, 홍빛 열무 그리고 무 종자를 파종한 뒤 새싹들을 광조건(20°C, RGB 6:1:3, 130μmol ·m-2·s-1, 12시간 광주기), 암조건 아래에서 각각 7일간 재배하였다. 처리 후 4일째부터 7일째까지 1일 간격으로 샘플을 채 취하였고 생체중과 건물중, 초장, 총 페놀 함량, 항산화도를 측정하였다. Brassica 종(케일, 다채, 브로콜리, 적양배추)과 Medicago 종(알팔파)은 암조건에서 생체중 값이 유의적으로 높았지만 생리활성 물질 함량은 광조건에서 유의적으로 증대 되었다. 이에 반해 Raphanus 속(홍빛 열무, 무)는 광조건에서 생체중이 유의적으로 증대되었지만 생리활성 물질 함량은 암 조건에서 유의적으로 높은 값을 나타내었다. 다양한 새싹 채소의 생육과 생리활성 물질 함량 사이에서 음의 상관관계가 관찰되었다. 본 연구는 다양한 새싹 채소의 생육 및 영양학적 가치에 대한 광의 영향을 확인하였고 고품질의 새싹 채소를 생산하는데 있어 수확시기의 중요성을 강조한다.

It is valuable research area regarding to developing manufacturing process of biogas from organic wastes take a side view of alternative for fossil energy and to improve the environmental atmosphere leads to decrease the greenhouse gas be discharged. The regulation which is prepared by environmental department shows that biogas as a transportation fuel is required to purity of above 95%, while it of natural gas is above 88%. However, in this situation it is necessary to prepare the regulation which is distributed by department of transportation as for the fuel be connected to vehicles as well in order to take step forward, and it is suggest to develop the technics of biogas this country's own original one, not be the technics imported from foreign countries, and also to turn to higher practical use of biogas for transportation area, since it shows far much less ratio have compared to other application areas.

Biodiesel is one of the most important renewable energy, since it is one of the most important alternative and eco-friendly energy source compared to fossil energy, especially for diesel engineequipped vehicles. It is now coming up for preparation of biodiesel as the alternative that applies the hetrogeneous catalyst, super critical state and various methods used in order to reduce the stages of the processes be converted and separation stages started from oil and fat which are originated from animals and wastes, and those new methods bring eco-friendly as well, though some of those are stand on research step so far. It is necessary that nation provides the tax exemption program for the supplier and strengthen the research system for the farming crops relate to fat and oil. It can improve the development of biodiesel which sustains stability against oxidation and keeps good mobility over the low temperatures in the winter season by new additives and develops new processes to show good harmony with diesel engine.

The electrochemical analysis of silver ion was performed using cyclic voltammetry (CV) and square-wave (SW) stripping voltammetry, and electrode cell systems were fabricated with graphite pencil electrode (GE) of working, reference and counter electrodes. Also electrolyte was the use of sea water as electrolyte solutions instead of ionic controlled solutions. The optimum analytical conditions for the cyclic and stripping parameters were determined using GE. The results approached the microgram working ranges of SW(ug/L) and CV(ug/L) Ag, and the optimum conditions were applied to frog's tissue and the food samples.

A voltammetric assay of phenol ions was investigated using three electrode systems of graphite pencil working, reference and counter electrodes. Under optimum analytical parameters, square wave stripping working ranges were attained at a mili range of 10~80 mg/L and a micro range of 20~90 ug/L using seawater electrolyte. The developed sensor was applied to tap water and the human body system of a smoker. It was found that the methods can be applied to in vivo fluid or medicinal diagnosis.