To evaluate effects of ligninolytic enzyme type on the mycelial response and ligninolytic enzyme production during interspecific interactions among wood-rotting fungi, 4 fungal strains, Trichophyton rubrum LKY-7, Trichophyton rubrum LSK-27, Pycnoporus cinnabarinus, and Trichoderma viride, were selected. Regarding ligninolytic enzyme production, LKY-7 secreted laccase and manganese peroxidase (MnP), P. cinnabarinus secreted only laccase, and LSK-27 secreted only MnP in glucosepeptone medium, while T. viride did not produce any ligninolytic enzymes. In the co-culture of LKY-7 with P. cinnabarinus, the formation of aerial mycelium was observed and the enhancement of laccase activity owing to interspecific interaction appeared to be very low. In the co-culture of LKY-7 and P. cinnabarinus with LSK-27, a hypha-free clear zone was observed, which resulted in deadlock, and increased laccase or MnP activity was detected at the interaction zone. The interaction responses of LKY-7, P. cinnabarinus, and LSK-27 with T. viride were characterized by the formation of mycelial barrages along the interface. As mycelial barrages were observed at the T. viride territory and no brownish pigment was observed in the mycelial barrages, it is suggested that laccase and MnP are released as part of an offensive response, not as a defensive response. The co-culture of P. cinnabarinus with T. viride lead to the highest enhancement in laccase activity, yielding more than 14-fold increase in laccase activity with respect to the mono-culture of P. cinnabarinus. MnP activities secreted by LKY-7 or LSK-27 was generally low in interspecific interactions.

We introduce a simplified crossing wood drain disperses rain water concentration and is used to protect the road surface from erosion due to flooding over the unpaved road. The efficiency of a simplified crossing wood drain was also investigated. A structure of simplified crossing wood drain can be produced within 10 minutes and installed within 18 minutes. The cost of the this product is 1/5 of that of the existing crossing drain product. The production and installation cost can be reduced according to dexterity. In the context of such applications, the degrees of damage for a rubber pad, which preventing the rain overflow, were varied according to their materials. A type of 8.2㎜ thick fabric rubber was the most suitable in this study.

In case an average accumulation of the investigated area is 200㎥/ha with cutting age of 80 years applied, annual workload will be 77ha and require 5 teams; each team consisting of 5 persons, enabling stabled supply of lumbers of 15,500㎥ every year. If one cutover is less than 2ha with cutting age of 80 years applied, it's possible to protect the peculiar ecology and secure stabled labor, workload and lumbering. It may become model forest out of tropical forest management which is controversial all over the world. Of course this presupposes construction of access roads.