The hypopharyngeal gland (HPG) of the honeybee worker produces royal jelly (RJ) and has a developmental cycle closely related to the division of labor. In this study, we investigated to compare the HPG acini diameter of differently aged worker bees with high royal jelly producing colony (HRC) or less producing colony (LRC). Additionally, we also evaluated whether the fresh weight of the head is a reliable indicator of the developmental status of HPG. The HRC showed a significantly higher RJ production about two-times as compared with those of the LRC. We measured the HG-diameters on days 1, 3, 6, 9, 12, 15. The microscopic analysis revealed that the acini size of the HRC was significantly larger than the LRC. In addition, the acini diameter of HRC was 15% longer than the LRC on the first day after emerging. It was shown that the fastest development during 3 days which is preparing for nurse the brood. The HPG acini diameters increased in both colonies in a similar fashion until day 12 and then decreased. We also compared the fresh head weight of the experimental colonies, differences were similar to the development of HPG. Therefore, high royal jelly production may have a positive correlation between HPG acini size and the fresh head weight.

The Riptortus (stinkbug) has a specialized symbiotic organ, M4 midgut, to harboring symbiont Burkholderia. M4 midgut is located in abdomen and surrounded with insect hemolymph. Recently our group demonstrated that symbiotic Burkholderia showed different physiology after adapting in M4 gut compare with in vitro cultured Burkholderia. And population of symbiotic Burkholderia in the M4 midgut is regulated by special organ. However, the molecular mechanism to prevent spreading and migrating symbiont bacteria to other host tissues from symbiotic organ is not clear. Therefore, we assumed that symbiont Burkholderia are susceptible to host humoral immunity after established infection in M4 midgut to prevent spreading and migrating into the other host tissues through Riptortus hemolymph. To prove this assuming, we tested the susceptibility and survival rate of symbiont Burkholderia in hemolymph of Riptortus in vitro and in vivo. We also examined the susceptibility of symbiont Burkholderia using purified antimicrobial peptides (AMP), pyrrhocoricin-like, thanatin-like and defensin-like AMPs. Finally, we tested inducing ability for AMPs by systemic infection of symbiotic Burkholderia. Gene expression of purified AMPs was not different after systemic infection of both symbiont and in vitro cultured Burkholderia. Surprisingly, in vitro cultured Burkholderia resisted on bacteria injected hemolymph and purified AMPs but symbiont Burkholderia were highly susceptible in bacteria injected hemolymph and purified AMP. These results suggest that symbiont Burkholderia can't survive in the hemolymph after escaping symbiotic organ. Moreover, humoral immunity of host Riptortus is important to prevent spreading and migrating symbiont Burkholderia into the other host tissue or organ from symbiotic organ.

The purpose of this study was to investigate the relationship between bone mineral density(BMD), body composition and life styles of female college students. The subjects were 334 students with a mean age of 21.0 years. BMDs of forearm and calcaneus were measured by dual energy X-ray absorptiometry, and body composition was measured by bioelectrical impedence analysis. The means for weight, height, and body mass index(BMI) of the subjects were 55.7 ㎏, 161.0 ㎝, 21.5 ㎏/m2, respectively. Grouping by the BMI, 13.2% of the subjects were classified as overweight, and 11.1% of the subjects as obese group. BMD in the forearm and calcaneus were 0.390 g/cm2, 0.514 g/cm2. In the forearm, 58.1% and 9.6% of the subjects were classified as osteopenia and osteoporosis, respectively, and in the calcaneus, 12.0% and 0.3% of the subjects were classified as osteopenia and osteoporosis, respectively. Body weight, skeletal muscle mass, lean body mass, fat mass and BMI were positively correlated with BMDs in the forearm and calcaneus(r=0.180~0.495, p<0.01~0.001), and height, % body fat and waist-hip ratio(WHR) were positively correlated with BMDs in the calcaneus(r=0.213~0.239, p<0.001), but not in the forearm. The factors such as beginning age of cigarette smoking and alcohol drinking and exercise had significant influence on BMD(p<0.05; p<0.001), whereas sleeping hours, activity level, frequency of exercise, meal times, frequency of snack and fried food intake, breakfast skipping and amount of meal had not significant influence on BMD. Therefore, the acquisition of balanced body composition is necessary through the increase of fat free mass and muscle mass, not through the increase of fat mass among female college students.

The purpose of this study was to investigate foodservice management practices in the child care centers and kindergartens. Interviews were held from May to July 2008 to understand the current situation concerning foodservices in Seoul and Gyeonggi provinces. Surveys were sent out from August 2008 to April 2009 to 1, 478 child care centers and 299 kindergartens via the postal service. Among them, 203 child care centers(13.7%) and 64 kindergartens(21.4%) responded. One of the largest concerns while preparing the food was nutrition(68.7%, 69.8%, respectively) followed by sanitation(24.4%, 27.0%, respectively). The most frequently referred sources for planning the menu were the Seoul child care information center(55.4%) for child care centers and kindergarten related internet sites(39.0%) for the kindergartens. In general, the child care center principal was in charge of planning the menu(40.1%). Child care centers and kindergartens purchased ingredients mostly from large retailers(55.3% and 44.7%, respectively), whereas 46.0% and 56.3% of those did through foodservice suppliers. Dietitians were rarely employed at these facilities, and this may cause unprofessional foodservice management.

This studyevaluates the effectiveness ofthe “ Seoul Sanitation Grading System Evaluation Index" devel0ped earlier and to analyze sanitation management practlces in restaurants in Seoul, Korea. The categories evaluated were the food management standard, facilities/equipment standard, and essential checking items specified in the law. These items were graded and classified into A (100~90)， B (89~80)， C (79~ 70) and Score (less than 69) based on the criteria set by the present researchers. We randomly selected 56 restaurants in five l0cal cities (Jung-gu, Seocho-gu, Jongno-gu, Songpa-gu and YIeongdeungpo-gu) and investigated each by actually visiting the site of business. The achievement rate for food management standard was 80.8%; as for the specific items in the category, it was the highest in food ingredients at 77.1 % and the lowest in food storage at 62.1 %. For the facilities/equipment standard, the achievement rate was 77.8%; as for the specific items in the category， it was the highest for vermin at 88 .1 % and the lowest for operation at 70.8%. The achievement rate for overall individual sanitary management was 70.7% and in the category, the lowest score was seen in hand washing at 57.1 %. The overall average score of sanitation management practices using the Seoul Sanitation Grading System Evaluation Index in restaurants in Seoul was 73.7, which fell into the C category. As for the number of restaurants in each grade category, there were 10 (17.9%) in each category of A (100~90), B (89~80) and C (79~70) with 30 (53.6%) scoring higher than 70, whereas those scoring less than 69 included 26 (46.4%). The average scores for those restaurants designated by local governments (exemplary restaurants, general restaurants, best Korean restaurants in Seoul) were not significantly diffierent; however, they were higher in franchises than those small restaurants ran by individuals.