The Lentinula edodes is the third most widely produced mushroom in the world. It is a rich source of proteins, fats, carbohydrates, fiber, vitamins, minerals and β -glucan. The aim of the this study was to evaluate the physicochemical characteristics changes such as proximate composition, minerals, and contents of β -glucan according to the different product area. And we evaluated the antioxidant properties of L. edodes. Also, we determined the standardization of manufacture process for commercial products using L. edodes. Eleven L. edodes were cultivated in oak, and samples were collected by different product area. And the L. edodes cultivated in sawdust have been producted in Jangheung. All samples are shade dried and those dried samples were used for this test.

Many Wild Mushrooms were collected at Yuchi recreational Forests areas for 1 day in July 2010 and 3 days in August 2010. They were identified and examined with references. According to the result, Species diversities are 1 division, 2 classes, 3 subclasses, 5 orders, 13 familys, 20 genera and 28 species. Dominant famillie are Tricholomataceae. Dominant genus are Amanita. Dominant species are Amanita verna (Bull. : Fr.) Roques and Coriolus versicolor (L. : r.) Quel. Resources of Wild Mushrooms were 2 species in edibility, 3 species in clulture, 12 species in toxine, 5 species in medicine, 2 species in anticancer, 11 species in ectomycorrizahe and 4 species in rotten wood.

Ionizing treatments were applied at 5Gy, 10Gy, 50Gy, 100Gy and 500Gy to mushroom mycelium (Lentinula edodes) in order to assesss the effect of the gamma-ray radiation. We mutated by gamma irradiation, 12 strains were isolated. 9 strains of 12 strains were appeared antagonistic interaction on solid medium. Growth rate of mycelium in JMIR-3 and JMIR-6 strains were similar to their control. But, the growth rate of strain JMIR-1, JMIR-2, JMIR-4, JMIR-5, JMIR-7, JMIR-8, JMIR-9, JMIR-10, JMIR-11 and JMIR-12 had generally different compared to control. In the sequence of ITS region of selected strains, it was revealed that the total length ranged from 696 to 780bp. The reciprocal homologies of the ITS region sequences in 5Gy irradiation group (JMIR-5, JMIR-6, JMIR-7 and JMIR-8) and 10Gy irradiation group (JMIR-9, JMIR-10, JMIR-1 and JMIR-12) indicated 99%. The reciprocal homologies of the ITS region sequences in 500Gy irradiation group (JMIR-1, JMIR-2, JMIR-3 and JMIR-4) indicated from 95 to 99%. It seemed that mycelium growth and ITS sequence could be changed the irradiation dose of the gamma-ray radiation.