혈액액비 시비가 채소 묘의 생육 및 품질에 미치는 영향을 구명 하기 위해 실험을 수행하였다. 토마토와 고추 그리고 겨자채를 무 비와 유비에 각각 파종하였으며, 무비에 혈액액비를 시비하여 채소 묘의 생육변화를 비교 분석하였다. 유비 육묘에서 토마토의 초장, 생체중, 그리고 엽폭이 각각 20.5±0.6cm, 51.1±2.6g, 6.1±0.2cm 로 가장 높았으며, 엽수는 유비, 무비, 그리고 혈액액비 시비에서 각 각 14.9±0.8, 9.2±0.4, 18.1±0.5 개로 혈액액비를 시비하였을 때 가장 많았다. 고추는 초장(9.1±0.3 cm), 생체중(30.2±1.6 g), 그리 고 엽폭(2.2±0.1 cm) 등은 모두 유비 육묘에서 생육이 가장 좋았으 며, 혈액액비를 시비하였을 때 무처리구 보다 생육이 향상되었다. 고추의 엽수는 유비구 7.9±0.2, 무비구 5.1±0.2개로 혈액액비 시 비구에서 8.2±0.1으로 가장 높았다. 겨자채 묘의 경우 전체적으로 유비 처리구에서 높은 결과를 나타 내었으며, 혈액액비 처리구가 겨자채 묘의 생육에 큰 효과를 보이진 않았다. 전반적으로 유비 육묘에서 생육이 가장 좋았으나, 혈액액비를 시비한 육묘에서도 생육 이 향상되었다. 본 연구 결과 가축의 혈액액비는 도축장의 폐기물 에 의한 환경오염을 줄 일 수 있고, 관행비료를 대체하여 친환경 유 기농 묘 생산에 사용 가능할 것으로 사료된다.

Investigation of flowing time, flower structure, microspore density, microspore vitality and microspore-derived embryo (MDE) formation rate according to the light quality treatment on broccoli donor plant was accomplished. The material was 08-8-3 line yielding high MDE production rate having 4.0 ± 0.5 mm flower bud length. The donor plant was cultivated with light quality treatment of red LED light, red+blue+white LED light and fluorescent light. The light intensity was 50 μ molm-2s-1 and photoperiod was 16/8 hours (light/dark). The flowering time was fastest at red LED light treatment compared to the other light treatment condition. 100.0, 36.4 and 18.2% of flower bud with longer stigma length than floral leaf which reported high MDE production rate were found under red LED light, Red+Blue+White LED light and fluorescent lights respectively. The microspore density and MDE production rate per single flower bud was highest at Red LED light. Suitable flower bud and high MDE production rate could be achieved in a short period if using LED light to broccoli donor plant cultivation. The above result is thought to be very useful for the development of a new cultivar of broccoli and other many crops including Brassica using haploid breeding technology. This journal was supported by the National Research Foundation

Heat shock pretreatment, dark culture period and washing medium could have marked effects on microspore embryogenesis. A heat shock pretreatment of microspores at 32.5°C for 48 hours gave high production rate of microspore-derived embryo (MDE) when compare to shorter and longer period. The yield of MDE increased significantly when microspore cultured for 15 days at 25℃ in dark condition followed by heat shock pretreatment. MDE were browned and lost vitality when dark treatment period extended longer than 15 days. This is caused by an insufficient oxygen and light for growing embryo which already formed during dark treatment period. The vitality of a microspore isolated from flower bud stored at 4℃ become decreased at the very first day and the vitality of microspore stored at 4℃ in the form of flower bud itself become decreased from the 5th day after storage. This shows the possibility of getting a certain period of storage for a suitable flower bud in MDE formation. The yield of MDE was most effective when isolated microspore was had with MS medium compared to B-5 and NLN medium and also showed most effective result with sucrose 130 g􀂂L-1 in additional sucrose concentration. The above result is thought to be very useful for the development of a new cultivar of radish and other many crops including Brassica using haploid breeding technology.