중금속이 곤충에 미치는 영향을 조사하기 위하여 빨간집모기 (Culex pipiens pallens) 금속용액 (Cu, Cd, Hg, Pb, Zn)에서 사육한 후 50% 치사농도 (LC50) 용화시기, 총 지질함량, 지질조성, 총 단백질 함량변화를 측정하였다. 50% 치사농도 (LC50)는 3령 유충으로 24시간 처리한 Hg 처리군에서 0.45 mg kg-1으로 나타나 다른 금속에 비해 독성이 강한 것으로 나타났고, 용화 시기는 처리군이 대조군 (129시간)에 비해 지연되었으며 Cd가 처리군 중 273시간으로 가장 늦게 용화되었다. 중금속에 노출된 모기시료에서 총 여섯 종류의 지질 band가 분리되었으며, 이 중 5종의 지질이 동정되었다 (phospholipid, cholesterol, fatty acid, triglyceride, cholesterol ester). 모든 처리군의 총 지질함량은 대조군에 비해 감소하였다. 또한, 대조군의 단백질 함량 (0.51 mg ind.-1)이 처리군의 단백질 함량에 비해 높게 나타났으며, 처리군 중 Hg과 Cd을 처리한 유충의 단백질 함량이 매우 낮게 나타났다. 결론적으로 중금속이 처리된 용액에서 사육된 모기유충은 오염된 환경에 의한 먹이섭취의 장애 및 중금속의 독성으로 인해 단백질 함량과 지질의 감소가 일어나 발육이 느려지고 용화시기가 길어지는 것으로 생각되며 이는 중금속에 의한 체내 물질대사의 변화를 의미한다. 이러한 결과는 중금속 오염이 모기의 생존과 성장을 억제시켜 모기 개체군 크기에 영향을 줄 것을 나타내며, 추후 연구를 통해 모기의 생육정도를 이용한 오염도 측정에 활용이 가능할 것으로 기대된다

To investigate the oviposition preference and development of azuki bean weevil (Callosobruchus chinensis L.), the following six different leguminous seeds were used in this study: red bean (Vigna angularis (Willd.) Ohwi & Ohashi), black soybean (Glycine max (L.) Merr.), soybean (Glycine max (L.) Merr.), seoritae (Glycine max (L.) Merr.), small black bean (Rhynchosia nulubilis) and kidney bean (Phaseolus vulgaris var. humilis Alef.). In the study of oviposition preference, the numbers of eggs per leguminous seed on red bean, black soybean, soybean, seoritae and small black bean were 1.23, 0.61, 0.69, 1.05 and 1.13, respectively. The maximum daily number of eggs was observed at 48 hours and the minimum was at 96 hours. According to each host leguminous seed, developmental time for each host seed was different. The shortest adult emergence time was on red bean (25.27 days). The other five leguminous seeds increased or doubled the adult emergence time. Adult emergence rates feeding on red bean, seoritae, black soybean, soybean, small black bean were 83.33%, 28.23%, 27.87%, 20.44%, and 11.59%, respectively. Emergence rate on red bean was four times higher than the rate on other seeds. The longevity of emerged female adults was almost all longer than that of males. The male adults weighed the lowest of feeding on small black bean. Female adults weighed the lowest of feeding on soybean. Adult weights were the heaviest for both males and females feeding on red bean. As a result, hosts of azuki bean weevil could decrease oviposition rate, emergence rate, adult longevity, and adult weight but increase emergence time. Especially in kidney bean, adult was not completely emerged. No eggs were laid. These results suggest that there might be emergence inhibitors in kidney bean. These imformation might be used to control damages caused by azuki bean weevils.

In the present study, lichen(Parmelia sp.) extract showed insecticidal activity against Aedes albopictus, and the effect of growth inhibiting activity was investigated. Acetone and methanol extracts of the lichen against larvae of Aedes albopictus were showed high insecticidal activities in low lethal concentration. 50% lethal concentration of the acetone extractof the lichen is 0.13% and the 50% lethal concentration of methanol extract of lichen is 0.15% respectively. This experiment that used acetone and methanol extracts of the lichen were observed for 24, 48 and 72 hours. As a result, the higher concentration and the longer exposure time is increased mortality against Aedes albopictus. Pupation time took more time as the higher concentration of acetone extract of lichen. Consequently, the lichen extract is effect in inhibiting the growth of Aedes albopictus larvae. In this experiment indicates that lichen extract has activity against Aedes albopictus and is available as the natural insecticide.

The purpose of this study was to evaluate the effect of pesticides on insect pest control of Rhophalosiphum nymphaeae, growth characteristies and dry root yield from the cultivated after early maturing rice cropping. All pesticides treated had no effect on the growh period, flowering rate of Alisma plantago. The major pesticides were methomyl-Lf, 24.1 %, imidacloprid-Wp, 10%, carbosulfan-Wp, 20%, and methomyl-Wp, 45%. Dry yield of root were increased largely with imidacloprid-Wp, 10% (10g/20~ell ), pesticide than the other pesticides and control. All pesticides were had no injury with recommended concentration. On the other hand all pesticides were slightly harmful in the double dosage level for the Alisma plantago.