Oleanolic acid is a natural triterpenoid that exists widely in foods and some medicinal herbs. The purpose of this study was to determine the antimicrobial activity of oleanolic acid against Streptococcus mutans strains isolated from a Korean population. Antimicrobial activity against these bacteria was evaluated by minimal inhibitory concentration (MIC) and time kill curves. The tolerance of human gingival fibroblasts and human periodontal ligaments to oleanolic acid was tested using a methyl thiazolyl tetrazolium (MTT) assay. The MIC90 value of oleanolic acid for both S. mutans and S. sobrinus isolated from Koreans was 8μg/mℓ. Oleanolic acid showed bactericidal effects against S. mutans ATCC 25175T and S. sobrinus ATCC 33478T at 1 × MIC(8μg/mℓ) and had no cytotoxic effects against KB cells at this dose. The results suggest that oleanolic acid could be useful in the future development of oral hygiene products for the prevention of dental caries.

목적: 이 연구는 중학생 선수들을 대상으로 케어링 분위기와 삶의 만족도 관계에서 라이프스킬 전이의 매개효과 를 분석하였다. 방법: 연구 참여자는 중학교 운동선수 221명(남자=155명, 여자=66명)으로 평균 15.94세였다. 측정 도구로는 케어링 분위기 척도(CCS), 라이프스킬 전이 척도(KLSTS), 그리고 삶의 만족도 척도(SWLS)가 사용되었 으며, 수집된 자료는 Stata 9.2를 이용하여 기술통계, 상관관계, 구조모형 분석을 실시했다. 결과: 첫째, 케어링 분 위기는 라이프스킬 전이에 유의한 영향을 미치는 것으로 나타났다. 둘째, 라이프스킬 전이는 삶의 만족도에 유의 한 영향을 미치는 것으로 나타났다. 셋째, 케어링 분위기는 삶의 만족도에 유의한 영향을 미치지 않았다. 넷째, 케어링 분위기와 삶의 만족도 관계에서 라이프스킬 전이는 매개효과가 있는 것으로 나타났다. 결론: 지도자를 포 함한 팀의 구성원들이 서로를 존중하고 격려하는 분위기는 스포츠에서 개발된 라이프스킬을 다양한 환경으로 전 이하는 데 보탬이 된다. 나아가 전이된 라이프스킬은 삶에 대한 선수들의 전반적인 만족감을 높이므로 지도자는 스포츠 환경 내에서 긍정적인 분위기를 형성할 필요가 있다.

Melatonin has several known physiological functions, the main one being synchronization of daily and seasonal rhythms. In addition, melatonin has been reported to influence reproduction and behavioral rhythms with varying results depending on the species. To date, it remains unknown how this rhythm in locomotor activity is controlled endogenously, although there must be coordination of chemical and molecular drivers. However, the species is poorly characterized at molecular level with little sequence information available in public databases. The aim of study was to clarify involvement of endogenous melatonin rhythms and locomotor activity in day-night activity of the eel, Anguilla japonica which is an economically important but endangered species. The levels during daytime (zeitgeber time; ZT 6) were significantly (P<0.05) lower than those during nighttime (ZT 18). A similar pattern was persisted under DD conditions, whereas it disappeared under LL conditions and ocular melatonin levels remained low. Therefore, it is likely that ocular melatonin levels of the nocturnal eel reared under LD and DD conditions fluctuate in a daily/circadian manner and night-related physiological processes are dependent on eel locomotor activities which is a nocturnal species. We found that similar number of genes were differentially expressed between day (ZT6) and nighttime (ZT18), suggesting that during the nighttime also important in differential gene expression with daytime. This work also provides essential information for further studies investigating the molecular basis of daily/circadian system in this species.