We have surveyed monthly diversity and seasonal prevalence of Macrolepidoptera in Wol-ak-san [Mt.] for the period of four months from May to August in 2010. Although it is not a part of KLTER/ME and KLPS study in Wol-ak-san conducted in recent few years, this year's data may be comparable with the previous ones as the collection sites and dates are very similar. The three representative vegetations are Quercus mongolica-, Quercus variabilis- and Pinus densiflora-formations, abbreviated as QM, QV and PD, respectively, and we collected exclusively larger moths with bucket-type of light traps for quantified survey. In 2010, as a result, QV showed higher species diversity and specimen number than QM or PD although the differences between QV and QM are very subtle. The result is very similar to the result in 2009. The only difference in 2010 over 2009 is that the number of species, not specimens, has been increased 1.5 times, and this may be caused by the inclusion of July records, which has been usually deleted due to heavy rainy season causing poor collection records. The number of species in Noctuidae was the highest in 2010, about twice more than that in 2009, but the dominant species in 2010 were either Pyralidae (Nacoleia commixta or Bradina geminalis) or Geometridae (Arichanna melanaria). We are still experiencing decline of specimen numbers: 567 specimens collected in 2010 while 651 in 2009 and 874 in 2008, which was already a huge reduction compared to over 2500 specimens in 2007.

With the help of KLTER/ME and KLPS in Woraksan, we have surveyed monthly diversity and seasonal prevalence of Macrolepidoptera in Wol-ak-san [Mt.] for the period of five months from May to September since 2005. Since 2007, to reduce variation, we chose two monitoring points, instead of one, for each of the three representative vegetations: Quercus mongolica-, Quercus variabilis- and Pinus densiflora-formations, abbreviated as QM, QV and PD, respectively, and collected exclusively larger moths with bucket-type of light traps for quantified survey. In 2009, as a result, QV showed higher species diversity and specimen number than QM or PD although the differences between QV and QM are subtle. The number of individuals collected may not be a good indicator, e.g., Idaea biselata collected in QM in June was over 200 in 2007, which is more than twice the number of specimens in all other sites per month, while the number in 2008 was dropped to only 10 specimens. In 2009, they became a dominant species again, showing biennial dominancy trend. It also seems that we are experiencing major decline of specimen numbers: 651 specimens collected in 2009 while 874 specimens collected in 2008, which was already a big reduction compared to over 2500 specimens in 2007

Cloned calves derived from somatic cell nuclear transfer (SCNT) have been frequently lost by sudden death at 1 to 3 month following healthy birth. To address whether placental anomalies are responsible for the sudden death of cloned calves, we compared protein patterns of 2 placentae derived from SCNT of Korean Native calves died suddenly at two months after birth and those of 2 normal placentae obtained from AI fetuses. Placental proteins were separated using 2-Dimensional gel electrophoresis. Approximately 800 spots were detected in placental 2-D gel stained with coomassie-blue. Then, image analysis of Malanie III (Swiss Institute for Bioinformatics) was performed to detect variations in protein spots between normal and SCNT placentae. In the comparison of normal and SCNT samples, 8 spots were identified to be up-regulated proteins and 24 spots to be down-regulated proteins in SCNT placentae, among which proteins were high mobility group protein HMG1, apolipoprotein A-1 precursor, bactenecin 1, tropomyosin beta chain, H+-transporting ATPase, carbonic anhydrase II, peroxiredoxin 2, tyrosine-rich acidic matrix protein, serum albumin precursor and cathepsin D. These results suggested that the sudden death of cloned calves might be related to abnormal protein expression in placenta.