Osteoarthritis is a disorder characterized by a loss of cartilage as common aging-associated disease in humans and animals. However, unlike human clinical trials, investigational studies in pet animals are constrained by a lack of interest and funds. In addition, pet owners would often prefer the lowest cost method to treat arthritis of pet animals. Here, we report the outstanding and inexpensive way to prepare chondrocytes for cartilage repair using rabbit adipose derived mesenchymal stem cells (MSCs). This study focused on the development and enhancement of pre-chondrogenic condensation under external electric fields even without additional growth factors. We found that highly compact structures were formed within 3 days in micromass cultures of rabbit MSCs under electrical stimulation (ES), showing increased COL2A1 gene expression compared with their control 3D micromass cultures and 2D monolayer cultures. We further found that ES enhanced the production of proteoglycan, a highly produced extracellular matrix component in chondrocytes. Collectively, these results provide the commercial potential of electrical stimulation driving chondrogenesis of mesenchymal stem cells for repair of cartilage, which is a budget-friendly regimen.

하천수사용 관리를 적정하게 관리하기 위해서는 객관적인 하천수사용량 자료가 수집되어야 한다. 그러나, 하천수 사용자의 취수여건을 고려하여 유량계 외에 수문조작 등의 간접적인 계측방법을 인정하고 있고, 사용자의 자발적인 보고에 의존함에 따라 자료의 객관화가 어려운 실정이다. 이에 한강홍수통제소에서는 농업용수 사용 비중이 커 하천유량 파악이 어려운 만경강의 고산~봉동 수위관측소 구간에 위치한 어우보 취수로에 V-ADCP를 이용한 계측시설을 설치 및 운영하고 있다. 본 연구에서는 V-ADCP로 측정된 유속을 이용하여 실시간 하천수사용량 산정을 위한 유속분포법의 적 용성을 평가하였다. 이를 위해 Chiu의 2차원 유속분포식의 매개변수 민감도를 분석하고, 실측유량 자료에 기초한 최적 매개변수를 산정하였다. 또한 수위-유량관계법, 지표유속법과 비교 평가하여 유속분포법의 특성을 분석하였다.

The purpose of this study was to improve the functionality of a healthy drink with examining the possibility of manufacturing different enzymes (alpha-, beta-, glucose-amylase) in barley malts (BM) produced in various malting periods. The study showed that enzyme treatment increased significantly total polyphenol content (TPC), DPPH radical scavenging activity and hydroxly radical scavenging activity in malted liquid samples (MLS) which obtained from various malting periods. The highest of TPC were found in Gluco-24M with 1.981 ㎎TAE/㎖, followed by Beta-24M and Alpha-72M with 1.878 ㎎TAE/㎖ and 1.845 ㎎TAE/㎖, respectively. The DPPH result revealed that percent of inhibition increased by 71-75% compared to the control. No statistical difference was found between MLS obtained by 24 hr of malting (24 M) and 72 hr of malting (72 M) after enzyme treatment. In addition, an increasing of hydroxyl radical was in the same trend to the TPC and DPPH. The hydroxyl radical scavenging activity of enzyme treated samples was 1,5 times higher than the control. These results suggest the possibility of enzyme application to barley malts obtained in various germination periods for improving quality and functionality of barley malts.