3The goal of this study was to develop high value mushroom foods possessing functionality. The effect of the Pholiota adiposa on the alcohol fermentation and its antihypertensive angiotensin I-converting(ACE) inhibitory activity of Korean traditional rice wine, Yakju was investigated. The ACE inhibitory activity of traditional rice wine was increased about 8% by the addition of 0.1% of the edible mushroom Pholiota adiposa ASI 24012 fruiting bodies into the mash containing 1% Lycii fructus, cooked rice, koji and antihypertensive S. cerevisiae and its Pholiota adiposa PAD-022 which showed anticholesteromia HMGCoA reductase inhibitory activity. Its quality characteristics and stability were investigated during the storage of room temperature and 40℃ for 3 months. It showed very high acceptability and also was very stable at 40℃ for 3 months[This study was supported by a grant from ARPC, 2005-2007].

3The goal of this study was to develop high value mushroom foods possessing functionality. The effect of the Pholiota adiposa on the alcohol fermentation and its antihypertensive angiotensin I-converting(ACE) inhibitory activity of Korean traditional rice wine, Yakju was investigated. The ACE inhibitory activity of traditional rice wine was increased about 8% by the addition of 0.1% of the edible mushroom Pholiota adiposa ASI 24012 fruiting bodies into the mash containing 1% Lycii fructus, cooked rice, koji and antihypertensive S. cerevisiae and its Pholiota adiposa PAD-022 which showed anticholesteromia HMG-CoA reductase inhibitory activity. Its quality characteristics and stability were investigated during the storage of room temperature and 40℃ for 3 months. It showed very high acceptability and also was very stable at 40℃ for 3 months[This study was supported by a grant from ARPC, 2005-2007].

β-Hydroxy-β-methylglutaryl coenzyme A reductase(HMG-CoA reductase, E.C. 1.1.1.34) is known to be rate-limiting enzyme in cholesterol biosynthesis. HMG-CoA reductase inhibitor is very useful in the remedy or precaution of hyperlipidemia. The methanol extracts of Pholiota adiposa ASI 24018 showed the highest HMG-CoA reudctase inhibitory activity of 55.8%. The HMG-CoA reductase inhibitor of Pholiota adiposa ASI 24018 was maximally extracted when it was treated with methanol at 30℃ for 12hrs. After purified the HMG-CoA reductase inhibitor by systematic solvent extraction, LH-20 gel column chromatography and RP-HPLC, finally obtained the HMG-CoA reductase inhibitor with an activity of IC50 6.8ug. Molecular weight of the purified HMG-CoA reductase inhibitor was 765.4Da by LC-Mass, NMR, FT-IR, UV spectrometry.