This report is a part of research on pests occurring in grapevine orchards in export complexes (Hwangsung in Gyeonggi, Sangju and Yeongcheon in Gyeongbuk, Namwon in Junbuk and Yeongdong in Chungbuk) from 2010 to 2012. This research was conducted to evaluate the distribution and difference in damage rates depending on management types of grapevine orchards (domestic sale farm vs. export farm). Damage by Arbordia spp. occurred only in 2010 and differed depending on localities and individual farms in the same area. Numbers of orchards damaged by Arbordia spp. were one, two and four in Hwasung, Namwon and Sangju, respectively, and the damage rate was below 6.2%. There was no damage in the orchards in 2011 and 2012, however, Arbordia spp. were collected on sticky traps in the orchards. A. nigrigena and A. kakogawana were the dominant species in Yeongcheon and Yeongdong, respectively, in 2011. A. kakogawana, A. maculifrons and A. nigrigena were collected on sticky traps in 2012. Collected numbers of Arbordia spp. were different depending on localities and management types of the orchards (domestic sale vs. export). A. kakogawana was the dominant species in all the survey sites and the densities were higher in the domestic sale farms than in the export farms.

The black cutworm, Agrotis ipsilon (Hufnagel), damages various cultivated crops and is also occasionally a serious pest of turfgrass, especially on golf courses. Essential oils have potential as alternative control agents for insect pests. Sixteen essential oils (anise, camphor, cinnamon, citronella, clove, fennel, geranium, lavender, lemongrass, linseed, neem, peppermint, pine, thyme, turpentine and tea saponin) and paraffin oil were assessed in the laboratory and the green house for their efficacy against black cutworm larvae. Treatment of potted perennial ryegrass with anise, cinnamon, neem, paraffin or turpentine reduced black cutworm damage in a greenhouse trial. Neem oil (2000 ppm) reduced growth of black cutworms feeding on treated clippings within 3 and 5 days. Treatment of perennial ryegrass in pots resulted in 100, 100 and 46% mortality of black cutworm at 4000, 2000 and 1000 ppm, respectively. Weight of survivors at the 1000 ppm rate was 5 fold less than weight of comparably-aged controls.

The nematicidal activity of Phellodendron amurense rhizome-derived materials (methanol extract) toward Meloidogyne Spp. second-stage juveniles (J2) and these effects on Cucumis sativus and Cucumis melo. Results were compared with these of fosthiazate. J2 was examined using 24-well plate tests, pot bioassays (C. sativa and C. melo) and Field trials (C. melo). In 24-well plate test with J2, methanol extract of P. amurense exhibited 98.7% and 69.8% mortality at 0.25 and 0.125 mg/ml toward J2, respectively, whereas Fosthiazate showed 100% mortality at 1 mg/ml. In pot bioassays with J2, P. amurense rhizome methanol extract gave 79.5% and 57.4% mortality at 2ℓ/m2(1,000x) and 2ℓ/m2(2,000x)/3kg soil from C. sativa and 73.7% and 53.3% mortality at 2ℓ/m2(1,000x) and 2ℓ/m2(2,000x)/3kg soil from C. melo, respectively. In Field test at C. melo in greenhouse showed 55.1% and 26.9% mortality at 2ℓ/m2(1,000x) and 2ℓ/m2(2,000x) applied soil. P. amurense rhizome-derived materials, merit further study as potential root-knot nematode control agents because of their nematicidal activity.

The Black pine bast scale, Matsucoccus thunbergianae is one of the most serious in black pine, Pinus thunbergii forests in Korea. Since this pest was first reported in Goheung, Korea in 1963, which is gradually spread into neighboring regions and now occurs in many regions of the southern and eastern part of the Korean peninsula. The monitoring for distribution of M. thunbergianae was able to observed by naked eye egg sacs and pupa of male on the host until now. Therefore, this monitoring was very difficult in the low density of M. thubergianae. This experiment was conducted to use simple and practical moving cross-shaped flat trap for monitoring of M. thunbergianae. The monitoring of M. thunbergianae using the device was carried out to southern regions of the Korean peninsula. The first emergence of male showed mid. March in Namhae and late march in Busan, Jinju and Pohang. The peak of emergence showed late March in Namhae and early April in the other regions. When the number of M. thunbergianae intermediate nymph showed 58~59, 11~44 and 8~25 on 39.25 ㎠ bark area of the black pine, Pinus thubergii for 1 week, the number of captured its male adult was 58~83, 67~488 and 1~55 on the moving cross-shaped flat trap (10× 13㎝), respectively. The low density of M. thunbergianae was some few the number of capture, but there were no significant difference in its high density. Also, the number of captured its male adult was no significant in the different color (yellow, red, white and blue) of the moving cross-shaped flat trap.

Pine wood nematode (PWN), Bursaphelenchus xylophilus is associated with the pine wilt disease and transmitted by pine sawyer, Monochamus alternatus. Because pine sawyer has one-year life cycle, one natural infection of PWN is occurred a year. Therefore, artificial propagation method of PWN is needed to improve experiment associated with PWN. In this study, effect of diameter, paraffin sealing of twig and dosage on pine wood nematode reproduction in Japanese black pine, Pinus thunbergii. PWN reproduction was compared in twigs of P. thunbergii and P. densiflora. Numbers of reproduced PWN were higher with decreasing diameter of twig. Distance (5 and 10 cm) from inoculation site of PWN did not influence reproduction of PWN. Reproduced numbers of PWN were higher in the paraffin-sealing twig than non-sealing twig. Dosage of PWN influenced reproduction of PWN. Reproduction rate was the highest at the rate of 10 IJs (13.7 and 61.1-fold increasing in P. densiflora and P. thunbergii, respectively 30 days later) whereas lowest at the rate of 1000 Ijs (1.1 and 0.7-fold increasing in P. densiflora and P. thunbergii, respectively 30 days later). Numbers of reproduced PWN were more in P. thunbergii than P. densiflora.

Host plants of insect pest in turfgrass and ornamental tree, Popillia flavosellata (Coleoptera: Ruteridae) were investigated in golf courses and forest. Twenty six species in 18 families were observed to be fed on from this research and 22 species in 15 families were recorded newly host plants of P. flavosellata. Thus, total number of host plants of P. flavosellata were 49 species in 23 species. P. flavosellata adult mainly fed on flower of host plants. Seasonal prevalence of P. flavosellata adult was investigated in East Valley Country Club in Gwangju, Gyeonggii, Korea using Japanese beetle pheromone lure and a eugenol feeding attractant. Peak activity of P. flavosellata adult was nearby 20 June. Mean attracted number of P. flavosellata adult at peak day were 197.6 individuals/ trap/day at 2008 and 268.1 individuals at 2010. Occurrence of P. quadriguttata were very lower then P. flavosellata. Mean attracted number of P. quadriguttata adult at peak day were 17.5 individuals/trap/day at 2008 and 2.3 individuals at 2010 in same trap.

Two new species of Tylenchorhynchus Cobb, 1913 from sugar cane fields in Sudan, T. sudanensis and T. elamini are described and illustrated. T. sudanensis is characterized by rather medium body size female, L= 0.692 ㎜ long with rather medium stylet length of 20 – 23 ㎛. While T. elamini is characterized by medium body size female, L = 0.61 – 0.78 ㎜ long, medium stylet length of 16 –19 ㎛. Different characters of two new species are discussed. In addition, vital measurements and brief descriptions of two species are presented.

The most effective methods against pine wilt disease (Bursaphelenchus xylophilus, PWD), black pine bast scale (Matsucoccus thunbergianae), pine needle gall midge (Thecodiplosis japonensis) is the trunk injection of insecticides. The period of trunk injection that was considered with ecology and physiology of pests such as pine wilt disease, black pine bast scale and pine needle gall midge, was applied from Dec. to next Feb. and June, Dec. respectively. And there are differences in quantities of being injected chemicals to pine trees by the period of trunk injection. Thus, we investigated to prevent the effect of insecticides, to estimate the effect under the trunk injection of insecticides for pine trees against pests by the injecting period and time, boring site, opening and shutting of injection site. On Pinuns thunbergii, it was examined to figure out the effect of insecticides by the injecting period, twice a month on a sunny day around 10 AM from January to September. Injecting of insecticides was tested at a dose of 5ml per cm dbh of a pine tree after boring with hand drill at 50 cm high from the ground. As a result, abmectin 1.8% EC and phospamidon 50% SL were injected over 90% of mortalities from the end of January to the middle of March but as time goes by, injecting insecticides tended to decrease. To compare the volume of injecting insecticides in a day, between 10 AM and 2 PM on February 23rd, abamectin 1.8% EC was injected at a dose of 5ml per pine trees with micro pipette after boring with a drill machine at 50 cm high from the ground. As a result, injecting insecticide of a pine which was injected around 10 AM was completely injected and about 90% of the insecticide was injected in case of a pine that was injected around 2 PM. Trunk injection of insecticides was examined to figure out differences of the volume of injected insecticides before and after rain. There was no difference not only the rainfall but also the bored direction.

Trunk injection of nematicides is applied to prevent pine wilt disease(PWD). Although the trunk injection of nematicides is effective to PWD prevention, it is not effective to vector insects such as adults of M. alternatus and M. saltuarius. Thus, This study was investigated for the insecticidal activity and the effect of feeding deterrence by injecting to pine trees with acetamiprid. For screening effective nematicides, some insecticides were injected to pines on December 30th, 2008 and their insecticidal activities and feeding areas of M. alternatus and M. saltuarius were investigated in the insects breeding dishes (100× 40mm) where put on 1 to 2-year-old twig which was selected from injected pine trees in early June. As a result, insecticidal activity and feeding deterrence of acetamicprid were most effective. Feeding areas of M. alterantus adults, when tested with 1-year-old twig and 2-year-old twig of healthy pine trees, were 170.3±107.0 mm2 and 258.0±123.2 mm2 respectively. And Feeding areas of M. alterantus adults, when tested with 1-year-old twig and 2-year-old twig of pines injected at a dose of 0.3 ml per cm dbh of a pine tree with acetamiprid, were 57.7±72.3 mm2 and 65.7±51.2 mm2 respectively and their mortalities also were 0%, 28.6%. The feeding areas of M. alterantus adults tested with 1-year-old twig and 2-year-old twig of pine trees injected at a dose of 0.6 ml per cm dbh of a pine tree with acetamiprid, were not fed on it and the mortalities were 28.0%, 57.1% respectively. In case of the feeding areas of M. saltuarius adults, when tested with 1-year-old twig and 2-year-old twig of healthy pine trees, it was 112.±35.1 mm2 and 159.2±65.2 mm2 respectively. And the feeding areas of M. saltuarius adults, when tested with 1-year-old twig and 2-year-old twig of injected pine trees at a dose of 0.3 ml per cm dbh of a pine tree with acetamiprid were 0.61±0.0 mm2 and 8.1±12.4 mm2 and both mortalities were 23.1%. Although M. saltuarius adults, when tested with 1-year-old twig of a pine tree injected at a dose of 0.6 ml per cm dbh of a pine tree with acetamiprid, were not fed on it but M. saltuarius adults tested with 2-year-old twig of the same pine tree were 2.0±3.7 mm2 and their mortalities were 53.8%, 61.5% respectively. We investigated insecticidal activities, the effects of feeding deterrence of acetamiprid for vector insects where fed twigs of injected pines by stop up hole with a cork or not, and the trunk injection to pine trees at a dose of 0.5, 1 ㎖ per ㎝ dbh of a pine tree with acetamiprid on the early June. As a result, all of the vector insects were not fed and the feeding area was within 11 ㎟ and the mortality of M. alternatus was 70%.

창원의 단감 과수원에서 2000년 6월 하순에 해충에 의한 피해 잎에서 총채벌레를 채집하여 분류한 결과, 우리나라에서는 처음으로 보고되는 관총태벌레科(Phlaeothripidae)의 감관총채벌레(신칭)(Ponticulothrips diospyrosi)로 확인되었다. 이 종의 간략한 형태 및 생태학적 특성, 피해증상, 경남 도내에서의 분포현황을 마련하였다

Steinernematid와 heterorhabditid 선충은 쥐며느리에 침입하여 치사시킬수는 있었지만 효과는 없었다. Steinernema carpocapsae 포천 strain과 S. glaseri 동래 strain이 S. carpocapse All strain과 Heterorohabditis bacteriophora보다 효과가 있었으며, 병원성 발현에서 선충의 접종농도가 기주의 밀도보다 중요한 요인이었다.