Following a series of major breakthroughs in artificial intelligence (AI) technology, it is believed that the use of AI technology can fundamentally subvert many industries and business fields, one of which is marketing. For instance, AI is likely to become a key driver of how advertising and marketing activities are conducted (Qin and Jiang, 2019) and thus dramatically change marketing strategies and customer behaviors (Davenport et al., 2020).

With the rapid development of the rural economy and vigorous promotion of energy-saving technologies, it is found through a field investigation that the new rural housing in the northeast of China shows new changes on green, energy-efficiency, health, comfort and beauty. The new rural housing mainly applied the technologies of composite energy-saving wall, double or three layers of glass energy saving windows and the roof benzene board insulation, heated in winter by Kang with the fuel of straw, corn stalks and other bio energy, which brings prominent energy-saving effect and the improved indoor environment significantly. The only or main function of the Kang is no longer the heating facilities in winter, but to play its unique benefits of comfort and health for sleep as a bed. the highlights of cultural traditions and customs in Cold rural area, the changes of the Kang are smaller and more exquisite. In the future, northeast rural housing should integrate the technolgies of residential structures, materials and kang further to promote rural sustainable development of society, economy.

With the rapid development of the rural economy and vigorous promotion of energy-saving technologies, it is found through a field investigation that the new rural housing in the northeast of China shows new changes on green, energy-efficiency, health, comfort and beauty. The new rural housing mainly applied the technologies of composite energy-saving wall, double or three layers of glass energy saving windows and the roof benzene board insulation，heated in winter by Kang with the fuel of straw, corn stalks and other bio energy, which brings prominent energy-saving effect and the improved indoor environment significantly. The only or main function of the Kang is no longer the heating facilities in winter, but to play its unique benefits of comfort and health for sleep as a bed. the highlights of cultural traditions and customs in Cold rural area, the changes of the Kang are smaller and more exquisite. In the future, northeast rural housing should integrate the technolgies of residential structures, materials and kang further to promote rural sustainable development of society, economy.

Bacillus thuringiensis, an entomopathogenic bacterium belonging to the B. cereus group, harbors numerous extra-chromosomal DNA molecules whose sizes range from 2 to 250 kb. In this study, we used a plasmid capture system (PCS) to clone three small plasmids from B. thuringiensis subsp. kurstaki K1 using PCS which were not found in B. thuringiensis subsp. kurstaki HD-1, and determined the complete nucleotide sequence of plasmid pK1S-1 (5.5 kb). Of the six putative open reading frames (ORF2-ORF7) in pK1S-1, ORF2 (MobK1) showed approximately 90% aa identity with the Mob-proteins of pGI2 and pTX14-2, which are rolling circle replicating group VII (RCR group VII) plasmids from B. thuringiensis. In addition, a putative origin of transfer (oriT) showed 95.8% identity with those of pGI2 and pTX14-2. ORF3 (RepK1) showed relatively low aa identity (17.8-25.2%) with the Rep protein coded by RCR plasmids, however. The putative double-strand origin of replication (dso) and single-strand origin of replication (sso) of pK1S-1 exhibited approximately 70% and 64% identities with those of pGI2 and pTX14-2. ORF6 and 7 showed greater than 50% similarities with alkaline serine protease, which belongs to the subtilase family. The other 2 ORFs were identified as hypothetical proteins. To determine the replicon of pK1S-1, seven subclones were contructed in the B. t huringiensis ori-negative pHT1K vector and were electroporated into a plasmid cured B. thuringiensis strain. The 1.6 kb region that included the putative ORF3 (Rep1K), dso and ORF4, exhibited replication ability. These findings identified pK1S-1 as a new RCR group VII plasmid, and determined its replication region.

As we all know Ondol has been the most efficient and comfortable heating system of the rural residence in the Northeast of China for hundreds of years. But recently, through field survey, it was found that some country people replace the Ondol with homemade heating blindly. The result of this replacement is that heating effect of residence in winter is worse. And at the same time, it aggravates air pollution and increases heating payout. According to our real situation of energy sources reserve, environmental pollution and economic income of rural people, it is not only inpractical but also unreasonable, that in the northeast of China Ondol is replaced by homemade heating completely in winter. Ondol has its unique heating and hygienic efficacy. And it stands for cultural tradition and life custom of the country's cold areas. So we should improve Ondol combining resident structure and material. And it can make the Ondol system flourish with vigor and promote sustainable development of the country's traditional Ondol residence.