This study was conducted to evaluate Raphanus sativus extracts to methane reduction in rumen. Five different levels of R. sativus extracts were used to investigate the most effective dosing level for the decrease of methane production in the rumen. The rumen fluid was collected from a cannulated one Hanwoo cow (BW=450±30 kg) consuming 600 g/kg timothy and 400 g/kg concentrate. On fermentation day, rumen fluid was collected at 2 hr postfeeding R. sativus extracts was dosed to achieve final concentration of 0, 1, 3, 5, 7, and 9% respectively, to fermentation bottles containing the mixture of rumen fluid and McDougall’s buffer and 300 mg of timothy was added as a substrate. The fermentation was conducted for 3, 6, 9, 12, 24, 48 and 72 hr incubation time at 39℃ with shaking. In vitro ruminal pH values were measured normal range for ruminal fermentation. Dry matter disappearance was significantly higher (p<0.05) at 3 hr incubation time 1, 3 and 5% doses than that of control. The highest methane reduction was observed in 12 hr incubation time 5, 7 and 9%. The carbon dioxide emission was also significantly (p<0.05) lower than that of control at 12 hr incubation time 5, 7 and 9%. The total volatile fatty acid was no significant difference between control and all doses level at 12 and 24 hr incubation time. At 24 hr incubation time, the result of real-time PCR were indicated that M. archea was significantly lower (p<0.05) at all doses level comparing to that of control. In conclusion, R. sativus extracts were significantly decreased methane emission. R. sativus extracts were significantly lower (p<0.05) than that of control at 12 hr incubation time 5, 7 and 9% and no adversely effect in rumen pH, dry matter disappearance and total VFA.

This study was conducted to evaluate natural plant extracts for methane gas reduction in ruminants. Rumen fluid was collected from cannulated Hanwoo cow (450±30 kg) consuming 400 g/kg concentrate and 600 g/kg timothy. The 15 ml of mixture comparing McDougall’s buffer and rumen fluid in the ratio 2 to 1, was dispensed anaerobically into 50 ml serum bottles. Rumen fluid contents were collected and in vitro fermentation prepared control (timothy, 300 mg), ginseng, balloon flower, yucca plant, camellia, tea plant and ogapi extracts were added at the level of 5% against 300 mg of timothy as a substrate (v/w) and incubated for 3, 6, 9, 12, 24, 48, and 72 h. In vitro pH values range 6.55~7.41, this range include rumen titration. The dry matter digestibility was not differ between all treatments and control. Total gas emission was significantly higher (p<0.05) in ginseng and balloon flower treatments on 24 h than in control. Carbon dioxide emission was not differ all treatments on 9 h than in control and significantly higher (p<0.05) yucca plant, camellia and tea plant treatments on 12 h than control. Methane emission was not differ all treatments on 6 h than in control. The rumen microbial growth rate was significantly higher (p<0.05) in ginseng, balloon flower on 12 h and significantly higher (p<0.05) in ginseng, yucca plant, tea plant and ogapi treatments on 24 h than in control. Total VFA was significantly higher (p<0.05) in tea plant and ogapi treatments on 12 h than in control and significantly higher (p<0.05) in ginseng, balloon flower treatments on 48 h than in control. Acetic acid was significantly lower (p<0.05) in ginseng and balloon flower treatments on 24 h than in control. Propionic acid was significantly higher (p<0.05) in ginseng and balloon flower treatments on 48 h than in control. As a results, sixth natural plant extracts had no significant effect dry matter digestibility and negative on rumen fermentation, but not effect methane reduction.

현존하는 교량의 실제적인 거동에 대한 보다 정확한 예측방법의 개발은 보수보강이 필요한 교량에 예산이 집중될 수 있도록 하여 교량운영의 경제성 및 안전성 측면에서 매우 중요하다. 특히 교량의 형태와 설치 지역의 활하중의 특성을 고려하며 활하중에 교량이 반응하는 실제적인 거동을 파악하여 실제적인 교량의 내하력 평가 이외에도 평가대상 교량의 선정 및 평가의 우선순위를 결정하여 교량의 유지 보수에 사용되는 예산의 보다 효율적인 집행을 가능하게 할 수 있다. 이 연구에서는 교량 현장실험에서 얻어지는 결과를 신뢰성 해석에 반영하여 보다 실제적인 교량 안전성 평가의 방법론을 연구하였다. 17개의 강거더 교량에 대해 기존의 교량 실험 결과를 토대로 교량의 내하력을 평가하기 위하여 2단계의 신뢰성 해석을 수행하였다. 우선 대상교량에 대해 설계에 사용된 계수 및 공칭강도를 이용하여 신뢰성 해석을 수행하였으며 2단계 신뢰성 해석에서는 교량 실험 결과를 신뢰성 해석에 포함하였다. 해석 결과를 비교해 본 결과 교량실험을 통한 각종 구조적 계수의 불확실성 제거를 통해 교량의 안전성을 저해하지 않고도 대상 교량의 신뢰성이 대폭 증가하는 결과를 얻을 수 있었다.