The objective of this study was to compare the feeding effects of imported timothy hay, domestic Italian ryegrass silage (IRGS) and IRG dried by hot-air dry system (IRGHDS) under basic total mixed ration (bTMR) on rumination activity, milk production, and milk composition in lactating dairy cows. Eighteen Holstein dairy cows were divided into three groups: control (bTMR + imported timothy hay), treatment 1 (bTMR + IRGS) and treatment 2 (TMR + IRGHDS) groups. The study was conducted over a total period of 24 days, including a 10 days adaptation period and a 14 days main experimental period. The results indicate body weight was not significantly different between the three groups (p>0.05), and rumination time was significantly higher in the treatment 1 group compared with the control group (p<0.001). Milk fat, protein, and lactose were not significantly different between the three groups (p>0.05), however, somatic cell counts were significantly lower in the treatment 1 group compared with the control group (p<0.001). As a result of the economic feasibility analysis, it is expected that feeding TMR using IRGS and IRGHDS will increase profit by 402.8 won/day and 331.4 won/day per cow compared to imported timothy hay. Therefore, IRGS and IRGHDS can be used as substitutes for imported timothy hay in feeding lactating dairy cows.

The objective of this study was to investigate the comparison of feeding a total mixed ration (TMR) containing imported alfalfa hay and TMR containing hot-air dried domestic alfalfa on rumination activity, milk production, and composition in lactating dairy cows. Ten Holstein dairy cows were divided into two groups: control (imported alfalfa hay + TMR) and treatment (hot-air dried domestic alfalfa + TMR) groups. The study was conducted over a total period of 18 days, including a 13-days adaptation period and a 5-days main experimental period. The results indicated no differences in total digestible nutrient and net energy intake between the imported and hot-air dried alfalfa. Body weight was not significantly different between the two groups (p>0.05), however, total feed intake and rumination time were significantly and tendentially higher in the treatment group compared with control group, respectively (p<0.001; p=0.075). Milk yield was not significantly different between the two groups (p>0.05), however, milk fat (kg) and lactose (%) concentration were significantly higher in the treatment group compared with control group (p=0.016; p=0.02). This study confirms that feeding TMR with hot-air dried domestic alfalfa results in no differences of feed intake, rumination activity, and milk productivity. Therefore, it is considered that hot-air dried domestic alfalfa can be used as a substitute for imported alfalfa on lactating dairy cows.

This study was performed to investigate immune changes by comparing the proportion and function of immune cells in the blood under high-temperature period and convalescence temperature period in Holstein dairy cows. The experiment was conducted using Holstein dairy cows of five animals per group (60 ± 20 months old, 175 ± 78 non-day) from the National Institute of Animal Science at high-temperature period (THI: 76 ± 1.2) and convalescence temperature period (THI: 66 ± 1.3). Complete blood count results showed no change in the number of immune cells between groups. In the analysis using Flow Cytometry of PBMCs, no significant differences were observed among B cells, Helper T cells, cytotoxic T cells, and γδ T cells between groups. However, there was an increase in Th17 cells producing IL-17a, while Th1 cells decreased during the convalescence temperature period. The results of gene expression analysis using qRT-PCR in PBMCs revealed an increase in IL-10 during the convalescence temperature period, while a decrease in HSP70 and HSP90 was observed. In conclusion, the increased expression of IL-10 and the decrease in HSP expression suggest the possibility of a weak recovery from heat stress. However, the lack of observed changes in B cells, T cells, and other immune cells indicates incomplete recovery from heat stress during the convalescence temperature period.

본 연구는 국화과 추출물 첨가가 in vitro 반추위 발효성상 및 메탄 발생에 미치는 영향을 구명하고자 수행하였다. 반추위액은 cannula 장착된 한우 암소 1두에서 채취하였으며, 공시축의 사양관리는 timothy와 농후사료를 6:4 비율로 하였다. 배양액은 Mcdougall buffer 10mL, 위액 5mL 섞어 50mL serum bottle에 혐기상태로 분주하였다. 티모시 0.3g를 각각 넣고 국화과 추출물인 국화, 민들레, 씀바귀, 제비쑥, 해바라기를 기질의 5%를 첨가한 뒤 발효시간대별(3, 6, 9, 12, 24, 48 및 72시간)로 7처리 3반복 수행하였다. 배양액 pH값은 6.29~7.44으로 반추위 적정 pH범위에 속하였다. 건물 소화율은 발효시간대가 늘어날수록 처리구에서 대조구에 비해 유의적(p<0.05)으로 증가하였다. 총가스 발생량은 발효 48시간대에서 민들레, 씀바귀, 제비쑥, 해바라기에서 대조구에 비해 유의적(p<0.05)으로 높게 측정 되었다. 이산화탄소 발생량은 발효 48시간대에서 민들레, 씀바귀, 제비쑥, 해바라기에서 대조구에 비해 유의적(p<0.05)으로 높게 측정 되었으며, 메탄 발생량도 발효 48시간대에서 민들레, 씀바귀, 제비쑥, 해바라기에서 대조구에 비해 유의적(p<0.05)으로 높게 측정 되었다. 미생물 성장량은 발효 48시간대에서 제비쑥과 해바라기 처리구에서 대조구에 비해 유의적(p<0.05)으로 높게 측정되었다. 발효 48시간대 에서 Acetic acid 생성량이 민들레, 씀바귀, 제비쑥, 해바라기 처리구에서 대조구에 비해 유의적(p<0.05)으로 높았다. 발효 12시간대에서 Propionic acid 생성량은 씀바귀, 제비쑥, 해바라기 처리구에서 대조구에 비해 유의적(p<0.05)으로 높았다. 발효 24시간대에서 A/P ratio는 민들레, 제비쑥, 해바라기 처리구에서 대조구에 비해 유의적(p<0.05)으로 감소하였다. 본 연구의 결과에 의해 국화과 추출물 첨가가 반추위 발효에 부정적인 영향을 미치지 않는 것으로 생각되나 메탄 발생량을 감소시키는데 영향이 없는 것으로 생각된다.

본 연구는 탄닌 함유 식물 추출물을 이용하여 in vitro 반추위 발효성상, 미생물 성장 및 메탄생성에 미치는 영향을 알아보고자 수행하였다. 반추위액은 농후사료와 조사료(timothy)를 40:60의 비율로 급 여한 반추위 cannula가 시술된 한우 암소에서 채취하였다. 본 실험에 사용한 식물 추출물은 상수리나 무(Quercus acutissima), 밤나무(Castanea crenata), 참가시나무(Quercus salicina), 졸참나무 (Quercus serrata) 그리고 떡갈나무(Quercus dentata)를 사용하였으며, 식물 추출물은 한국식물추출물 은행에서 분양받아 사용하였다. 반추위액과 McDougall buffer를 1:2의 비율로 혼합한 배양액을 0.3g timothy와 식물 추출물(기질의 5%)이 담긴 50mL serum bottle에 혐기상태로 15mL를 분주하였다. Serum bottle은 39℃, 120rpm으로 3, 6, 9, 12, 24, 48 및 72시간 동안 배양하였다. 본 실험 결과 배 양 시간이 지날수록 pH는 점점 감소하였으며, 정상범위에 포함되었다. 건물소화율은 대조구에 비해 첨 가구에서 유의적(p<0.05)으로 낮았다. 암모니아, 총 가스 생산량 및 이산화탄소 발생량은 대조구에 비 해 첨가구에서 유의적(p<0.05)으로 차이가 없었으나, 24시간 메탄 발생량은 대조구에 비해 전 첨가구에 서 유의적(p<0.05)으로 낮았다. 미생물 성장량은 첨가구에 따라 각각 다른 양상을 나타내었고, acetic acid 및 propionic acid는 대조구에 비해 첨가구에서 유의적(p<0.05)으로 낮았다. Real-Time PCR에서 섬유소 박테리아(R. albus, R. flavefaciens) 및 메탄 생성균(Ciliate associated methanogen)은 대조 군에 비해 유의적(p<0.05)으로 감소하였다. 결과적으로 본 실험에 사용한 탄닌 함유 식물 추출물 5종 모두 반추위 발효에 영향을 미치지 않으며, 메탄저감 효과를 나타내었다. 특히 상수리나무에서 반추위 발효성상에 악영향을 미치지 않으며 메탄 발생을 저감하는 식물 추출물로 적합하다고 생각된다.

In this study, surface particulate matter (PM2.5) concentrations were calculated based on empirical equations using measurements of ceilometer backscatter intensities and meteorological variables taken over 19 months. To quantify the importance of meteorological conditions on the calculations of surface PM2.5 concentrations, eight different meteorological conditions were considered. For each meteorological condition, the optimal upper limit height for an integration of ceilometer backscatter intensity and coefficients for the empirical equations were determined using cross-validation processes with and without considering meteorological variables. The results showed that the optimal upper limit heights and coefficients depended heavily on the meteorological conditions, which, in turn, exhibited extensive impacts on the estimated surface PM2.5 concentrations. A comparison with the measurements of surface PM2.5 concentrations showed that the calculated surface PM2.5 concentrations exhibited better results (i.e., higher correlation coefficient and lower root mean square error) when considering meteorological variables for all eight meteorological conditions. Furthermore, applying optimal upper limit heights for different weather conditions revealed better results compared with a constant upper limit height (e.g., 150 m) that was used in previous studies. The impacts of vertical distributions of ceilometer backscatter intensities on the calculations of surface PM2.5 concentrations were also examined.

This study was conducted to evaluate roughage to concentrate ratio on the changes of productivity and metabolic profiling in milk. Six lactating Holstein cows were divided into two groups, T1 group was fed low-concentrate diet (Italian ryegrass to concentrate ratio = 8:2) and T2 group was fed high-concentrate diet (Italian ryegrass to concentrate ratio = 2:8). Milk samples were collected and its components and metabolites were analyzed by 1H-NMR (Nuclear magnetic resonance). The result of milk components such as milk fat, milk protein, solids-not-fat, lactose and somatic cell count were not significantly different between two groups. In carbohydrate metabolites, trehalose and xylose were significantly higher (P<0.05) in T1 group, however lactose was not significantly different between two groups. In amino acid metabolites, glycine was the highest concentration however, there was no signifi-cant difference observed between two groups. Urea and methionine were significantly higher (P<0.05) in the T2 group. In lipid metabolites, carnitine, choline and O-acetylcarnitine there were no significant difference observed between the two groups. In benzoic acid metabolites, tartrate was significantly higher (P<0.05) in T2 group. In organic acid metabolites, acetate was significantly higher (P<0.05) in T1 group and fumarate was significantly higher (P<0.05) in T2 group. In the other metabolites, 3-methylxanthine was only significantly higher (P<0.05) in T2 group and riboflavin was only significantly higher (P<0.05) in T1 group. As a result, milk components were not significantly different between two groups. However, metabolites concentration in the milk was significantly different depends on roughage to concentrate ratio.

This study was conducted to investigate for the natural methane emission inhibitor as a feed additive no adversely effect on rumen fermentation. Five different Control (Wheat barn (0.05 g), MRA(Methane Reduction Additive)-1 (Allium fistulosum L. (0.05 g)), MRA-2 (Sodium Lauryl Sulfate (0.025 g) +Wheat barn (0.025 g) mixed), MRA-3 (Sodium Dodecyl Sulfate (0.025 g) + Wheat barn (0.025 g) mixed), and MRA-4 (Allium fistulosum L. (0.02 g) + Tannic acid (0.02 g) + Wheat barn (0.01 g) mixed) contents were used to perform 3, 6, 9, 12, 24 and 48 h incubation for in vitro fermentation. Ruminal pH values were ranged within normal ruminal microbial fermentation. Dry matter digestibility was not significantly different across the treatments during the whole fermentation time. Also, the result of microbial growth had no adversely effect on during the whole fermentation time. At 24 h, methane emission was significantly lower (P<0.05) than all treatments except to MRA-1. Especially, MRA-4 carbon dioxide emission was significantly lower (P<0.05) than control at 9, 24 and 48 h incubation. In addition MRA-4 propionate concentration was significantly higher (P<0.05) than control at 24 h incubation. The result of RT-PCR Ciliate-associated methanogens were significantly lower (P<0.05) at MRA-1, MRA-3 and MRA-4 than control at 24 h incubation. Based on the present results, MRA-4 could be suggestible methane emission inhibitor as a natural feed additive.

This study was conducted to evaluate Raphanus sativus extracts to methane reduction in rumen. Five different levels of R. sativus extracts were used to investigate the most effective dosing level for the decrease of methane production in the rumen. The rumen fluid was collected from a cannulated one Hanwoo cow (BW=450±30 kg) consuming 600 g/kg timothy and 400 g/kg concentrate. On fermentation day, rumen fluid was collected at 2 hr postfeeding R. sativus extracts was dosed to achieve final concentration of 0, 1, 3, 5, 7, and 9% respectively, to fermentation bottles containing the mixture of rumen fluid and McDougall’s buffer and 300 mg of timothy was added as a substrate. The fermentation was conducted for 3, 6, 9, 12, 24, 48 and 72 hr incubation time at 39℃ with shaking. In vitro ruminal pH values were measured normal range for ruminal fermentation. Dry matter disappearance was significantly higher (p<0.05) at 3 hr incubation time 1, 3 and 5% doses than that of control. The highest methane reduction was observed in 12 hr incubation time 5, 7 and 9%. The carbon dioxide emission was also significantly (p<0.05) lower than that of control at 12 hr incubation time 5, 7 and 9%. The total volatile fatty acid was no significant difference between control and all doses level at 12 and 24 hr incubation time. At 24 hr incubation time, the result of real-time PCR were indicated that M. archea was significantly lower (p<0.05) at all doses level comparing to that of control. In conclusion, R. sativus extracts were significantly decreased methane emission. R. sativus extracts were significantly lower (p<0.05) than that of control at 12 hr incubation time 5, 7 and 9% and no adversely effect in rumen pH, dry matter disappearance and total VFA.

This study was conducted to evaluate natural plant extracts for methane gas reduction in ruminants. Rumen fluid was collected from cannulated Hanwoo cow (450±30 kg) consuming 400 g/kg concentrate and 600 g/kg timothy. The 15 ml of mixture comparing McDougall’s buffer and rumen fluid in the ratio 2 to 1, was dispensed anaerobically into 50 ml serum bottles. Rumen fluid contents were collected and in vitro fermentation prepared control (timothy, 300 mg), ginseng, balloon flower, yucca plant, camellia, tea plant and ogapi extracts were added at the level of 5% against 300 mg of timothy as a substrate (v/w) and incubated for 3, 6, 9, 12, 24, 48, and 72 h. In vitro pH values range 6.55~7.41, this range include rumen titration. The dry matter digestibility was not differ between all treatments and control. Total gas emission was significantly higher (p<0.05) in ginseng and balloon flower treatments on 24 h than in control. Carbon dioxide emission was not differ all treatments on 9 h than in control and significantly higher (p<0.05) yucca plant, camellia and tea plant treatments on 12 h than control. Methane emission was not differ all treatments on 6 h than in control. The rumen microbial growth rate was significantly higher (p<0.05) in ginseng, balloon flower on 12 h and significantly higher (p<0.05) in ginseng, yucca plant, tea plant and ogapi treatments on 24 h than in control. Total VFA was significantly higher (p<0.05) in tea plant and ogapi treatments on 12 h than in control and significantly higher (p<0.05) in ginseng, balloon flower treatments on 48 h than in control. Acetic acid was significantly lower (p<0.05) in ginseng and balloon flower treatments on 24 h than in control. Propionic acid was significantly higher (p<0.05) in ginseng and balloon flower treatments on 48 h than in control. As a results, sixth natural plant extracts had no significant effect dry matter digestibility and negative on rumen fermentation, but not effect methane reduction.

이 연구에서는 중소지간 합성형 강거더교량에 대한 신뢰성 해석을 위해 강거더와 콘크리트슬래브의 강성을 토대로 처짐을 고려한 한계함수를 구축하여 신뢰성해석을 수행하였다. 확률적 하중과 저항모델을 통해 처짐을 예측하기 위해 계산에 필요한 변수들을 확률변수로 고려하였다. 강재의 부식에 의한 단면의 감소, 그리고 콘크리트의 크리프는 합성형교의 처짐에 많은 영향을 미친다. 따라서 이 연구에서는AASHTO LRFD 기준으로 설계된 교량에 대해 시간에 따른 변수를 고려하여 강재단면의 감소와 크리프의 영향을 통계적 모델에 반영하기 위해 몬테-카를로 시뮬레이션 기법을 이용하였으며, 처짐과 사용성을 고려하여 다양한 지간과 거더간격을 가진 교량에 신뢰성 해석을수행하였다. 그 결과, 장지간 교량의 경우, 단지간 교량과 비교해 보았을 때 상대적으로 크리프와 강재단면감소의 처짐에 대한 영향이 작았으며, 이에 반해 단지간 거더 교량의 경우 크리프의 진행에 따라 처짐에 많은 영향을 미친다는 것을 알 수 있었다.

본 연구에서는 강거더 연속교에 대해 국내외 설계기준에 규정되어 있는 활하중으로 인한 충격계수를 실험을 통해 검증하였다. 대부분의 도로교의 설계기준에서는 도로교의 경우 충격계수를 약 0.3 정도의 값으로 규정하고 있으나, 연속교의 경우에는 명확한 규정 및 명시가 없는 상황이다. 그러므로 단순교에 적용되는 충격계수를 연속교에 동일하게 적용하는 것이 일반적이다. 이 연구에서는 현장실험을 통해 연속교의 충격계수가 단순교에 적용되는 경우와 같이 사용될 수 있는지를 검증하였다. 현장실험 결과 얻어진 충격계수는 2차선 교량에서 1대의 만재트럭이 통과했을 경우 가장 하중이 많이 작용한 거더에서 0.2 이내의 충격계수가 계측되었으며, 2개의 차선을 통시에 만재트럭이 통과한 경우 그 충격계수는 0.05 이내의 값을 얻었다.

The paper is focused on evaluation of the effect of quality of concrete and construction workmanship on the reliability of beams. The reliability analysis is performed for moments and shear forces in steel beams. The basic resisance parameters considered in this study are bias factor and coefficient of variation. The calculated reliability indices indicate that bias factor is the most critical parameter that determines the structural performance.

The objective of this research is to verify the code–specified girder distribution factors (GDF) for continuous steel girder bridges by field testing. it is found that the analysis results are not conservative, and in some instance, the analytical results underestimate the actual GDF’s, which can lead to a groundless notion of safety. As a result, it is found that GDF’s specified in AASHTO LRFD should be used with careful reservation.

이 연구의 목적은 재하 실험을 통해 교량설계기준에 규정되어 있는 횡방향 활하중 분배계수에 대해 연속교에 대해 적응이 가능한지를 검증하는 것이다. 기존의 유한요소해석을 이용한 해석적인 연구에서 설계기준에 규정되어 있는 횡방향 분배계수가 매우 보수적이며 정모멘트구역과 부모멘트 구역에서 활하중의 분배 형태는 유사하다는 결론을 얻었다, 재하실험을 통해 변형율을 계측하고 유한요소해석의 결과와 비교해 본 결과, 대부분의 경우 실험에서 얻어진 변형율이 해석 결과보다 작았다. 특히 이상적인 조건을 가정하고 행해진 구조해석 결과는 상대적으로 외측 거더에 작용한 휨모멘트를 과다 평가하였으며 따라서 구조해석 결과에서 얻어진 횡방향분배계수는 계측결과와 비교해 볼 때 보수적이지 않다는 것을 알 수 있었다. 실험 결과 또한 1개의 교량의 부모멘트구역에서 AASHTO LRFD에서 규정한 값을 초과하는 횡방향분배계수가 계측되었다. 따라서 AASHTO LRFD를 이용하여 연속거더교의 각 거더에 작용하는 활하중 모멘트 산정시에는 주의가 필요하다고 판단할 수 있다. 이에 반해 AASHTO Standard 식은 재하실험 결과 예외 없이 매우 보수적임을 검증하였다.

현존하는 교량의 실제적인 거동에 대한 보다 정확한 예측방법의 개발은 보수보강이 필요한 교량에 예산이 집중될 수 있도록 하여 교량운영의 경제성 및 안전성 측면에서 매우 중요하다. 특히 교량의 형태와 설치 지역의 활하중의 특성을 고려하며 활하중에 교량이 반응하는 실제적인 거동을 파악하여 실제적인 교량의 내하력 평가 이외에도 평가대상 교량의 선정 및 평가의 우선순위를 결정하여 교량의 유지 보수에 사용되는 예산의 보다 효율적인 집행을 가능하게 할 수 있다. 이 연구에서는 교량 현장실험에서 얻어지는 결과를 신뢰성 해석에 반영하여 보다 실제적인 교량 안전성 평가의 방법론을 연구하였다. 17개의 강거더 교량에 대해 기존의 교량 실험 결과를 토대로 교량의 내하력을 평가하기 위하여 2단계의 신뢰성 해석을 수행하였다. 우선 대상교량에 대해 설계에 사용된 계수 및 공칭강도를 이용하여 신뢰성 해석을 수행하였으며 2단계 신뢰성 해석에서는 교량 실험 결과를 신뢰성 해석에 포함하였다. 해석 결과를 비교해 본 결과 교량실험을 통한 각종 구조적 계수의 불확실성 제거를 통해 교량의 안전성을 저해하지 않고도 대상 교량의 신뢰성이 대폭 증가하는 결과를 얻을 수 있었다.