The objective of this study was to compare the feeding effects of imported timothy hay, domestic Italian ryegrass silage (IRGS) and IRG dried by hot-air dry system (IRGHDS) under basic total mixed ration (bTMR) on rumination activity, milk production, and milk composition in lactating dairy cows. Eighteen Holstein dairy cows were divided into three groups: control (bTMR + imported timothy hay), treatment 1 (bTMR + IRGS) and treatment 2 (TMR + IRGHDS) groups. The study was conducted over a total period of 24 days, including a 10 days adaptation period and a 14 days main experimental period. The results indicate body weight was not significantly different between the three groups (p>0.05), and rumination time was significantly higher in the treatment 1 group compared with the control group (p<0.001). Milk fat, protein, and lactose were not significantly different between the three groups (p>0.05), however, somatic cell counts were significantly lower in the treatment 1 group compared with the control group (p<0.001). As a result of the economic feasibility analysis, it is expected that feeding TMR using IRGS and IRGHDS will increase profit by 402.8 won/day and 331.4 won/day per cow compared to imported timothy hay. Therefore, IRGS and IRGHDS can be used as substitutes for imported timothy hay in feeding lactating dairy cows.

The objective of this study was to investigate the comparison of feeding a total mixed ration (TMR) containing imported alfalfa hay and TMR containing hot-air dried domestic alfalfa on rumination activity, milk production, and composition in lactating dairy cows. Ten Holstein dairy cows were divided into two groups: control (imported alfalfa hay + TMR) and treatment (hot-air dried domestic alfalfa + TMR) groups. The study was conducted over a total period of 18 days, including a 13-days adaptation period and a 5-days main experimental period. The results indicated no differences in total digestible nutrient and net energy intake between the imported and hot-air dried alfalfa. Body weight was not significantly different between the two groups (p>0.05), however, total feed intake and rumination time were significantly and tendentially higher in the treatment group compared with control group, respectively (p<0.001; p=0.075). Milk yield was not significantly different between the two groups (p>0.05), however, milk fat (kg) and lactose (%) concentration were significantly higher in the treatment group compared with control group (p=0.016; p=0.02). This study confirms that feeding TMR with hot-air dried domestic alfalfa results in no differences of feed intake, rumination activity, and milk productivity. Therefore, it is considered that hot-air dried domestic alfalfa can be used as a substitute for imported alfalfa on lactating dairy cows.

This study was performed to investigate immune changes by comparing the proportion and function of immune cells in the blood under high-temperature period and convalescence temperature period in Holstein dairy cows. The experiment was conducted using Holstein dairy cows of five animals per group (60 ± 20 months old, 175 ± 78 non-day) from the National Institute of Animal Science at high-temperature period (THI: 76 ± 1.2) and convalescence temperature period (THI: 66 ± 1.3). Complete blood count results showed no change in the number of immune cells between groups. In the analysis using Flow Cytometry of PBMCs, no significant differences were observed among B cells, Helper T cells, cytotoxic T cells, and γδ T cells between groups. However, there was an increase in Th17 cells producing IL-17a, while Th1 cells decreased during the convalescence temperature period. The results of gene expression analysis using qRT-PCR in PBMCs revealed an increase in IL-10 during the convalescence temperature period, while a decrease in HSP70 and HSP90 was observed. In conclusion, the increased expression of IL-10 and the decrease in HSP expression suggest the possibility of a weak recovery from heat stress. However, the lack of observed changes in B cells, T cells, and other immune cells indicates incomplete recovery from heat stress during the convalescence temperature period.

본 연구는 우리나라 Holstein의 번식형질에 대한 유전모수를 추정하기 위하여, 2009년부터 2013년까지 분만한 농협 젖소개량사업소의 검정자료 경산우 144,793두의 211,899개의 번식기록을 이용하였다. 7가지 형질의 유전 및 오차 (공)분산을 WOMBAT 패키지를 이용하여 분석하였다. CTFS, GL, NRR, NS, FSTC, DO 및 CI의 유전력은 각각 0.064, 0.060, 0.005, 0.014, 0.012, 0.021 및 0.021로 추정되었다. 형질들 간의 유전상관은 –0.910에서 0.995로 나타났다. NRR과 NS, NRR과 FSTC 간에는 부의 유전상관이 나타났으나, DC와 CI는 강한 양의 유전상관을 나타냈다. CTFS와 FSTC는 DO 및 CI와 유의하게 양의 유전상관관계를 나타냈다(0.479~0.862). CTFS와 FSTC 사이의 낮은 유전상관은 두 형질이 서로 다른 유전적 측면에 있다는 것을 의미한다. 본 연구는 번식능력 개량을 위한 기초 자료를 제공할 수 있을 것이라 판단한다.

This study was performed to investigate the effect of heat-stressed environment on rumen microbial diversity in Holstein cows. Rectal temperature and respiration rate were measured and rumen fluid was collected under normal environment (NE; Temperature humidity index (THI)=64.6) and heat-stressed environment (HE; THI=87.2) from 10 Holstein cows (60±17.7 months, 717±64.4 kg) fed on the basis of dairy feeding management in National Institute of Animal Science. The rumen bacteria diversity was analyzed by using the Illumina HiSeq™ 4000 platform. The rectal temperature and respiratory rate were increased by 1.5ﾟC and 53 breaths/min in HE compared to that in NE, respectively. In this study, HE exposure induced significant changes of ruminal microbe. At phylum level, Fibrobacteres were increased in HE. At genus level, Ruminococcaceae bacterium P7 and YAD3003, Butyrivibrio sp. AE2032, Erysipelotrichaceae bacterium NK3D112, Bifidobacterium pseudolongum, Lachnospiraceae bacterium FE2018, XBB2008, and AC2029, Eubacterium celulosolvens, Clostridium hathewayi, and Butyrivibrio hungatei were decreased in HE, while Choristoneura murinana nucleopolyhedrovirus, Calothrix parasitica, Nostoc sp. KVJ20, Anabaena sp. ATCC 33047, Fibrobacter sp. UWB13 and sp. UWB5, Lachnospiraceae bacterium G41, and Xanthomonas arboricola were increased in HE. In conclusion, HE might have an effect to change the rumen microbial community in Holstein cows.

본 연구는 우리나라의 Holstein 암소의 주요 번식형질과 305 일 산유량과의 상관관계를 알아보고자 실시했다. NRR 56d 와 305 일 산유량과의 상관관계를 알아보기 위해 2003 년부터 2013 년사이에 분만한 농협중앙회 젖소개량사업소 검정자료 경산우 479,331 두를 이용하였으며, 분만계절, 산차 및 유량그룹을 고정효과로 하는 Yijkl= u + seasoni + parityj + yieldgroupk + eijkl 선형모형을 적용하여 분석을 실시했다. NRR 과 305 일 산유량의 단순상관 및 오차상관은 각각 –0.089 및 –0.018 로 분석되어 산유량이 증가할수록 NRR 은 감소하는 것으로 나타났다. 이는 고능력우의 저수태 발생율이 높다는 주장을 뒷받침 한다. 다음은 최근 인공수정의 횟수가 증가하고 있는 현상이 유생산과 어떠한 관계가 있는지 알아보기 위해 수정횟수와 305 일 산유량과의 관계를 분석했다. 자료는 젖소개량사업소의 동일한 자료를 이용했다. 305 일 유량이 6000kg 미만, 6,000~7,000kg, 7,000~8,000kg, 8,000~9,000kg, 9,000~10,000kg, 10,000~11,000kg, 11,000~12,000kg, 12,000kg 이상, 8 개 그룹으로 나누어 305 일 유량에 따른 수정횟수를 분석했다. 각 그룹별로 평균 수정횟수는 각각 1.57, 1.59, 1.62, 1.66, 1.72, 1.78, 1.83, 1.91 회로 나타났으며 수정횟수 3 회 및 4 회 이상의 저수태우 발생비율 역시 산유량이 증가함에 따라 증가하는 것으로 나타났다. 다른 주요 번식형질인 분만간격과 305 일 산유량과의 상관관계를 알아보기 위해 젖소개량사업소 검정자료인 2010 년부터 2013 년 사이에 분만한 2 산차 이상 경산우 42,550 두를 데이터 분석에 이용했다. 분만간격은 361 일 미만, 361~400 일, 401~450 일, 451~500 일, 501~550 일, 551~600 일, 601~650 일 및 651 일 이상, 총 8 개 그룹으로 나누어 분석을 하였다. 305 일 추정유량은 분만간격이 길어짐에 따라 유의적으로 증가하는 것을 나타났다. 다음으로는 초산차 홀스타인 젖소에서 분만계절과 305 일 유량의 관계를 분석했다. 조사에 이용된 데이터는 2009 년부터 2011 년까지 분만한 1 산차 14,189 두의 농협중앙회 검정자료를 이용했다. 305 일 추정유량은 봄, 여름, 가을, 겨울이 각각 9,684, 9,413, 9,805, 10,084 kg 으로 여름철 분만이 겨울철 분만우보다 약 6.3% 낮은 것으로 나타났다. 유량 위주의 개량은 젖소의 번식형질을 저하시킨다는 연구가 다수 있었지만, 낙농 선진국과는 달리 국내에서는 아직 번식형질에 대한 유전평가는 실시하지 않고 있다. 이러한 국내 기초자료를 근거로 우리나라도 산유량 위주의 유전평가가 아닌 번식형질에 대한 유전평가도 필요한 시점이라 생각한다.

본 연구는 Holstein 암소 34두로부터 조사된 체고 측정 기록에 대해 세 가지 비선형 성장곡선을 적용하여 성장모형을 추정하고, 추정된 성장모형의 모수를 이용하여 Holstein 암소의 성장특성에 대한 기초자료를 제공하고자 실시하였다. Gompertz, von Bertalanffy 및 Logistic 모형으로 추정된 성장곡선 함수식은 각각 Ht=457.2e-1.705e-0.02552t, Ht=465.4(1-0.417e-0.0.01724t)3 및 Ht=1079.1(1+12.682e-0.04275t)-1이였다. 최대성장시기를 나타내는 변곡점( )은 Gompertz, von Bertalanffy 및 Logistic에 대해 각각 616.039, 419.253 및 1759.728일로 추정되었다. 변곡점에서의 체고 증가속도 추정치는 Gompertz, von Bertalanffy 및 Logistic에 대해 각각 0.138, 0.109 및 0.366cm으로 나타났으며, 변곡점에서의 체고는 각각 176.5, 137.9 및 539.5cm으로 추정되었다. 오차 평균 제곱합의 결과를 보면 Logistic, von Bertalanffy 그리고 Gompertz 모형 순으로 적합도가 좋은 것으로 판단된다.

This study was conducted to investigate the relationship between changes of rumen microflora and bloat in Jersey cow. Jersey cows (control age: 42 months, control weight: 558kg; treatment age: 29 months, treatment weight 507kg) were fed on the basis of dairy feeding management at dairy science division in National Institute of Animal Science. The change of microbial population in rumen was analyzed by using next generation sequencing (NGS) technologies due to metabolic disease. The diversity of Ruminococcus bromii, Bifidobacterium pseudolongum, Bifidobacterium merycicum and Butyrivibrio fibrisolvens known as major starch fermenting bacteria was increased more than 36-fold in bloated Jersey, while cellulolytic bacteria community such as Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens was increased more than 12-fold in non-bloated Jersey. The proportion of bacteroidetes and firmicutes was 33.4% and 39.6% in non-bloated Jersey’s rumen, while bacteroidetes and firmicutes were 24.9% and 55.1% in bloated Jersey’s. In conclusion, the change of rumen microbial community, in particular the increase in starch fermenting bacteria, might have an effect to occur the bloat in Jersey cow.

This study was conducted to estimate the growth parameters of Holstein cows and to compare their growth characteristics based on weight-age data of Holstein cows of 293 heads from National Institute of Animal Science in Korea. Three models(Gompertz, von Bertalanffy and Logistic model) were considered for analyses. The estimated growth functions for Gompertz, von Bertalanffy and Logistic models were Wt=781.0e−2900e−0.09521t,Wt=943.3(1−0.650e−0.06231t)3 and Wt=665.7(1+13.475e−0.18260t)−1 , respectively. The estimated average infection points(ti) showing maximum growth rate were 352.034, 338.181 and 430.987 day of age, respectively, using on Gompertz, von Bertalanffy and Logistic model. Average gain at inflection for Gompertz, von Bertalanffy and Logistic model were estimated 0.878, 0.833 and 0.986 kg/day, respectively. The estimated weight at inflection for Gompertz, von Bertalanffy and Logistic model were 301.5, 279.5 and 332.9 kg, respectively. In the study, the von Bertalanffy model having a variable point of inflection fitted the best for data of Holstein cows.