This study was conducted to find out the variation in agronomic trait and chemical composition in the collected Perilla frutescens from China and Japan. From the results of growth investigation, the maximum number if branches was 26.7ea in Japan 134 line, followed by 25 nodes number in China 119 line. Among the different lines investigated, maximum number of panicle number (108.8) were observed in China 114 line. 1000 seed weight was maximum (4.12 g) in China 118 line. Flowering time of different collected lines varied significantly with average value of 175.5 days and the average line required for maturation of seedlings was 205.1 days. Plant height was the highest (248.9 ㎝) in China 107 line. Highest number of total picking leaves was 965ea, and the average picked period was 54 days. The major phenol compounds contained in Perilla frutescens showed wide variation for Syringic acid, Benzoic acid, Naringin, o-Coumaric acid, Myricetin, Naringenin and Hesperetin. Japan 139 line showed the highest level of total phenol contents (8254.0 ㎍/g, dry weight).

Objectives The objective of our research was to establish the gene transformation, expression and characterization system in transgenic Codonopsis lanceolata. Materials and methods Agrobacterium tumefaciens strain LBA 4404/ binary vector pYBI121Regeneration of transgenic shoots: MS medium supplemented with 0.1 mg/ℓ NAA and 1 mg/ℓ BAP, 3% sucrose and 0.8% agar at pH 5.8. Agrobacterium cell density OD 600 between 0.8 and 1.0, Infection: 5 minutes DNA isolation and Polymerase chain reaction: DNA was extracted from young leafs excised from kanamycin resistant shoots. Two primers used for PCR amplification of the 700 bp of the npt II gene were N 1 (5′ GAA GCT ATT CGG CGG CTA TGA CTG 3′) as a sense primer and N 2 (5′ ATC GGG AGC GGC GGC GAT ACC CTA 3′) as a anti sense primer. Result and Discussion Adventitious shoots regenerated 3 weeks after Agrobacterium infection on regeneration medium containing 0.1 mg/ℓ NAA and 1 mg/ℓ BAP, 100 mg/ℓ kanamycin 250 mg/ℓ cefatoxime. Numerous adventitious shoot inductions of putative transformants were observed from the cut surface of explants which initially resembled knob like structure and later developed into new plant. PCR analysis of showed the expected bands of npt II gene. PCR analysis was carried out to confirm the insertion of the npt II gene in the genome of transformed plant. The expected amplified npt II fragments of size 700 bp was found in the T0 transformed plants, indicating the integration of npt II gene.