Nueva Ecija is a landlocked province in Central Luzon region that is composed of 27 municipalities and 5 cities. It is a province that is rich with biodiversity on its north and east borders, which exhibit a shared ecosystem with the Caraballo mountain range in the north and the Sierra Madre mountain range in the east. Its main economic activity is farming where rice is the main crop. Part of the Sierra Madre mountain range traverses its towns of Bongabon, Laur, Rizal and Pantabangan where most of the locals perform agro – forestry activities as a means of generating income for their families. Due to the accumulation of farm residues as a result of intensive agro-forestry activities, mushroom pharming becomes a very viable agribusiness undertaking in the area. Our research team initiated the establishment of database by tracking the mushroom production activities in the province. Similarly, the prevailing climatic condition in the geographical area and the type of agro-forestry wastes have been tracked. Wastes as a result of intensive agro – forestry activities are being generated which the locals oftentimes burned in the field that posed hazard to the environment. In order to discourage this practice, our research team generated technologies on mushroom pharming using rice straw as the main substrate. The promotion of mushroom pharming is based on the suitability of mushroom species to grow in climatic conditions that prevail in the specific geographical area in the province. As a result of its collaborative research undertakings, the Center for Tropical Mushroom Research and Development of the Central Luzon State University in collaboration with the Laboratory of Forest Products Chemistry of Tokyo University of Agriculture and the Nikken Sohonsha Corporation encouraged the adoption of medicinal mushroom production technologies on rice straw – based formulation. With oyster mushroom (Pleurotus spp) as the most widely cultivated mushroom among the mushroom growers in the province, other ligninolytic mushrooms such as lacquered mushroom (Ganoderma) and wood ear mushroom (Auricularia spp.) are grown by the locals. Similarly, leaf – litter degrading mushrooms such as paddy straw mushroom (Volvariella volvacea) are also being cultivated in the province. However, some mushroom growers in the province adopt sawdust – based mushroom production technology which is not economically appropriate in the province due to the scarcity of its availability compared to rice straw. Majority of the mushroom growers in the province adopt the rice straw – based mushroom technology. Moreover, high value mushroom – based products other than the fresh mushrooms are already in the local market.

Collybia reinakeana (P.) Henn used to be a wild endemic edible mushroom not until it was discovered in the mountainous area of Puncan, Carranglan, Nueva Ecija, Philippines after the great earthquake that jolted Central Luzon in 1990. It is characterized to having big fruiting bodies (about 1 foot tall) that grow in cluster. Its mycelia were rescued from the wild and the first domestication studies were conducted which lead to the generation of its production technology. It is a nutraceutical mushroom that exhibits antibacterial and anti-hypertensive properties aside from its appreciable content of both standard as well as non- standard amino acids. It also exhibits mycoparasitic habit against species of Aspergillus, Cladosporium and Fusarium. Recently, emergence of this mushroom in the different areas in the Philippines have been reported which prompted our research team to conduct geo - tracking in order to rescue its secondary mycelia in our desire to conserve this endemic species of mushroom. With our conservation efforts, we have tracked the areas where it was found growing and subsequently isolated the secondary mycelia. At present, our research team were able to collect 9 different strains from the different areas namely Bataan (1), Science City of Munoz (2), Urdaneta City (1), San Manuel, Pangasinan (1), Rosales, Pangasinan (1), Bicos, Rizal, Nueva Ecija (1), Dipaculao, Aurora (1) and Bamban, Tarlac(1).

Collybia reinakeana (P.) Henn used to be a wild endemic edible mushroom not until it was discovered in the mountainous area of Puncan, Carranglan, Nueva Ecija, Philippines after the great earthquake that jolted Central Luzon in 1990. It is characterized to having big fruiting bodies (about 1 foot tall) that grow in cluster. Its mycelia were rescued from the wild and the first domestication studies were conducted which lead to the generation of its production technology. It is a nutraceutical mushroom that exhibits antibacterial and anti-hypertensive properties aside from its appreciable content of both standard as well as non- standard amino acids. It also exhibits mycoparasitic habit against species of Aspergillus, Cladosporium and Fusarium. Recently, emergence of this mushroom in the different areas in the Philippines have been reported which prompted our research team to conduct geo – tracking in order to rescue its secondary mycelia in our desire to conserve this endemic species of mushroom. With our conservation efforts, we have tracked the areas where it was found growing and subsequently isolated the secondary mycelia. At present, our research team were able to collect 9 different strains from the different areas namely Bataan (1), Science City of Munoz (2), Urdaneta City (1), San Manuel, Pangasinan (1), Rosales, Pangasinan (1), Bicos, Rizal, Nueva Ecija (1), Dipaculao, Aurora (1) and Bamban, Tarlac(1).

[Introduction] Mushroom constituents have been found to be highly effective in the prevention and treatment of lifestyle diseases such as lipidosis, high blood pressure, and diabetes, which are closely linked to eating habits, and several varieties of functional foods have been developed from these constituents. As a result, doctors of Western medicine in particular, who in the past have been dismissive of herbal or Oriental medicine, are now tending to take a more proactive stance toward adopting the better aspects of alternative, complementary or traditional medicine. Natural remedies and folk medicines have been incorporated into the treatment of cancer, for example, and in the same way it has become common to incorporate mushrooms into treatment regimes before and after surgical interventions and alongside therapies such as chemotherapy, radiation therapy, and hormone therapy. Mushroom chitosan, which is investigated in this study, is a novel functional ingredient made from the mushroom Flammulina velutipes (Curt.:Fr.) Sing., which is the most common edible mushroom to be artificially cultivated in Japan and has long been part of the Japanese diet. Unlike chitosans of crustacean origin, mushroom chitosan is rich in the main structural component β-glucan, and this dietary fiber is expected to have positive functions within the body. In particular, there are hopes that mushroom chitosan will inhibit cholesterol and fat absorption in the small intestine, and suppress total cholesterol and neutral fat levels in the serum. Previous human trials have confirmed the anti-metabolic syndrome efficacy of supplements containing mushroom chitosan. Here we report the ameliorative effects of mushroom chitosan in an animal model of genetic obesity and lipidosis. [Methods] Mushroom chitosan (RSK2, Ricom Corporation) was administered at different doses to Crj:(ZUC)-fa/fa rats an animal model of obesity and hyperlipidemia continuously for 10 weeks. The rats were kept at a temperature of 22±1ºC and humidity of 60±10%, and illuminated with fluorescent lamps for 12 h/day (07:00~19:00). Body weight, food consumption, body condition, and hematological and blood biochemical blood parameters were measured, and pathological examination (pathological analysis of hepatic lipid droplets) was performed using HE staining. [Results and discussion] Mushroom chitosan (RSK2) showed high efficacy in suppressing weight gain in Crj:(ZUC)-fa/fa rats presenting obesity due to genetic lipidosis. The smallest effective dose was 3 mg/kg. In addition, values for neutral fat, β-lipoproteins and total lipid due to ingestion improved to the normal values. Moreover, the pathological study of the liver revealed a decrease in lipid droplets appearing in the central zone of the hepatic lobule and a decrease in fat deposition in the liver in the group that ingested mushroom chitosan. The results also suggested that serum lipid levels were improved through egestion of excess fat with the feces. Mushroom chitosan (RSK2) was shown to be effective in controlling increase in serum lipids as it has lipase-inhibiting activity, and was shown to control fat deposition in internal organs through egestion of excess blood lipids with the feces. Mushroom chitosan is thus a functional food that is effective in preventing and treating contemporary lifestyle diseases.