Cryopreservation of miniature pig sperm is essential because of high demand of organ transplant in mass production. However, miniature pig sperm are vulnerable to oxidative stress more than other mammals. Erythritol is a naturally occurring sugar alcohol with powerful antioxidant property. Thus, the aim of our study is to verify if erythritol could reduce lipid peroxide and enhance viability of frozen thawed miniature pig sperm. Ejaculated semen samples were frozen with cryoprotectant subjected to erythritol treatment (0, 10, 100, 500 mM). After frozen thawed, spematozoa viability were examined using the computer assisted sperm analysis (CASA) system. The product of lipid peroxidation, malondialdehyde (MDA) were quantified using spectrophotometer with DPPH and ABTS assays as ROS scavenger markers. Our result showed that erythritol enhanced sperm viability (p<0.05), reduced lipid peroxides significantly (p<0.05), proving the concentration of 100 mM erythritol to be an effective for lowing oxidative damage. Data from our study suggest that erythritol exhibits significant lipid peroxidation scavenging characteristics which may prevent oxidative damage, enhance viability of frozen thawed sperm and thus could be a effective additive as cryoprotectant.

The purpose of this study analyzed the overview of corrugated cardboasrd manufacturing industry and then provide direction for improvement. The BREF (BAT reference document) is an important reference for licensees and officer, including the best available techniques for the industry and achievable environmental performance, technical characteristics, and economic information. In the corrugated cardboard manufacturing process, wastewater pollutants are generated throughout the production process, and water is used in the dissociation and aging process. Atmospheric emissions are mostly generated by steam production from boilers and incinerators for the dry process. SO2, NOx, CO2, CO, HCl, dust, VOC, and odor were common. In the EU-BREF (European union BAT reference documents) BAT for wastewater have taken up a relatively large proportion. Items of water pollutants in wastewater were common in COD, BOD, N, P, SS, and however EU-BREF had different pollutants such as AOX and salt compared to K-BREF. In order to improve the quality of the K-BREF, it is necessary to devise basic data research method and data acqusitiom method. Consideration should be given to additional environmental management techniques that reflect the emissions characteristics of the corrugated cardboard manufacturing process. In addition, further research is needed to develop methodologies for selecting BATs considering environmental and economic feasibility.

The integrated permit system is applied to class 1 and 2 atmospheric and water pollutant discharge facilities in 19 sectors. The fertilizer sector should receive a permit for a period of four years, from 2019 to 2023. The purpose of this study is to investigate the differences between the Korean Best Available Techniques (BAT) reference document (K-BREF) and the European Union BAT reference document (EU-BREF) in terms of the process and emission characteristics of fertilizer manufacture. K-BREF is written by focusing on products, whereas EU-BREF is produced on focusing on manufacture process. There are five types of BATs(best available techniques economically achievable) in Korea. These BATs selected mainly to recover and reuse raw meterials and save energy. The number of BATs and BAT-AELs(BAT associated emission level) in K-BREF is smaller than that in EU-BREF. We suggest that BATs and those environmental management parameters in Korea need to further reflect the emission characteristics in the fertilizer sector.