The growth characterization and enzyme productivity of halotolerant microorganisms isolated from the Nakdong River estuary wetland in Korea were investigated in this study. The isolation of halotolerant microorganisms was carried out through their cultivation at 37oC for several days using a marine agar medium. After a single-colony isolation, 16 pure colonies were obtained, and phylogenetic analysis was done using 16S-based ID service in the EzBioCloud database. The isolated strains were divided into 3 phyla, 5 families, 7 genera, and 13 species. Possible industrial applications of the strains were confirmed upon testing for amylolytic, lipolytic, and proteolytic activity to confirm the possible production of hydrolytic enzymes, growth at a pH range of 5 to 9, and various salt concentrations being tested. Thirteen strains possessed at least one enzyme activity, and 5 strains possessed 2 enzyme activities. Eight strains could tolerate up to 10% NaCl concentration. This result indicated that isolated strains had shown the possible application in food and cosmetic industries. Therefore, this study would contribute to securing domestic biological resources and improving hydrolytic enzyme activity using these strains.

충청북도농업기술원에서는 연한 분홍색의 향기가 있고, 꽃수가 많으며, 개화기간을 오래 지속할 수 있는 속간 교배종 난 ‘핑키뷰티’를 육성하였다. 신품종 ‘핑키뷰티’는 2007년 Phal. pulcherrima × Phal. parishii 교배종을 모본으로, Neof. falcata ‘Hwacheon’을 부본으로, 속간교배하였다. 종자 발아, 재배, 선발, 특성검정은 2008년부터 2014년까지 진행되었다. 생육이 좋고 꽃의 색, 모양이 우수한 계통을 선발 한후, 2015년부터 2017년까지 3년에 걸쳐 국립종자원 신품종 심사기준으로 특성검정 결과, 우수성이 인정되어 2018년 최종 선발하여 “핑키뷰티”로 명명하였다. 주요 특성은 다음과 같다. 화색은 연분홍색으로 Red-purple Group 68D(RHS칼라차트)이며, 향기가 있고 화경색은 암록색이다. 식물체 크기는 12.7cm로 대조품종과 큰 차이를 보이지 않았다. 잎의 폭은 1.6cm로 대조품종인 P. pulcherrima에 비해 좁은 편이다. 화경장은 25.8 cm이며, 소화수는 16.3개로, 전반적으로 화분의 볼륨감이 우수한 편이다. 꽃의 크기는 2.5cm 정도이며, 꽃의 순판 뒤쪽에 풍란과 같은 짧은 거가 있는 것이 특징이다. 개화기는 5월 하순이며, 개화기간은 34일 정도이다.

하늘매발톱꽃(Aguilegia flabellata var. pumila K.) 육묘시 적정 용토종류 및 시비 수준을 구명코자, 용토종류는 원예용 상토, 피트모스, 밭흙, 원예용상토 + 피트모스(1:1, v/v), 원예용상토 + 밭흙(1:1, v/v), 밭흙 + 피트모스(1:1, v/v), 밭흙 + 퇴비(1:1, v/v), 원예용상토 + 피트모스 + 밭흙(1:1:1, v/v/v) 혼합용토를 사용하였으며, 시비 시험은 기준시비량을 질소 – 인산 – 칼리 = 12 – 9 – 12kg/10a(초화류 표준시비량)을 기준으로 하여 기준시비량 대비 0%, 50%, 75%, 100%, 125% 및 150%를 처리하여 시험한 결과, 용토종류는 용토별 화학적 특성은 피트모스가 pH 및 양분함량이 가장 낮았고, 원예용 상토 및 퇴비가 혼합된 용토에서 EC를 비롯한 양분함량이 높은 경향이었다. 발아율은 피트모스 단용 처리에서 파종 30일 및 45일에 각각 48% 및 54%로 가장 낮았고, 밭흙 + 퇴비 혼합용토에서는 86%였고, 그 외 용토에서는 92~98%였다. 파종 후 120일과 150일의 묘생육은 원예용상토에서 가장 좋았다. 시비량별 생육은 무처리에 비하여 시비 처리구에서 생육이 좋았으며, 식재 100일 후 생육에서는 기준 시비량 75%, 100% 및 125%에서 양호한 경향을 보였다. 기준 시비량 150% 처리에서는 오히려 생육이 감소하는 경향이었다. 결과적으로 하늘매발톱꽃 육묘시 육묘용토에서는 원예상토를 이용하고 시비기준은 기준시비량 대비 75%로 관리하는 것이 전반적인 생육이 양호하였다.

An earthquake of ML 5.8 hit the Gyeongju area on September 12, 2016. A sequence of foreshock-mainshockaftershock of 588 events with equal to or greater than magnitude 1.5 occurred for six months in this area. Around ninetynine percentage (98.8%) of the total energy was released intensively within a day, and about 80% of the total events took place within a month after the Gyeongju earthquake. The epicentral distribution of aftershocks of major events (ML 5.1, 5.8, 4.5, and 3.5) were elongated in the direction of N30 o E. They correlate well with the focal mechanism solution. These facts support the inference that the Gyeongju earthquakes occurred on a sub-parallel subsidiary fault of the Yangsan fault zone or on the linking damage zones between Deokcheon and Yangsan fault. During the last six years before the Gyeongju earthquake, there were few events within 10-km radius from the epicenter. This seismic gap area was filled with a sequence of the Gyeongju earthquakes. The b value for aftershock of the Gyeongju earthquakes is 1.09.

This study was conducted to develop gibberellin treatment technique to enhance flower initiation in Aquilegia japonica Nakai & H. Hara. Seedlings were planted in 12㎝-diameter pots on October 2016 and grown in green house. Ambient temperature in the green house was set at minimum 15℃ during day and night to suppress flower initiation at cold temperature condition. Two different types of gibberellin, GA3 and GA4+7, at 4 different concentration levels 100, 200, 400 and 600 ㎎/L, were tested in this study. Gibberellin was sprayed first at planting and secondly at 1-week after planting. Ten to fifteen ㎖ of gibberellin was sprayed for each pot. Plant height and petiole length were elongated by both gibberellin types, flowering was more enhanced by GA3 (91.7∼100%) compared to of GA4+7. However, abnormal flower was less observed in GA3 treatment (0∼16.7%) than GA4+7. Number of flower stalks per plant ranged from 1.9 to 2.5. Number of flowers per plant ranged from 6.8 to 10.3. Differences in flowering characteristics between treatments were statistically significant. Optimal gibberellin treatment to enhance flower initiation in A. japonica Nakai & H. Hara substituting cold treatment was GA3 at the concentration between 400 ㎎/L to 600 ㎎/L.