본 연구에서는 다양한 색상의 염료가 절화 장미 ‘Akito’ 품종의 염색에 미치는 효과를 알아보기 위해 시행되었다. 12가지 색상의 염료를 이용한 염색 결과, 다채로운 색상을 구현해 내고 관상 가치를 높일 수 있었지만 절화수명은 대조구보다 1-3일 단축되었다. 4종류의 blue 염색염료를 비교한 결과 Fantasy blue (Palace Chemical Co., Japan)와 Blue No.1(Vidhi Dyestuff Mfg LTD, India)의 색상이 좋았다. 고농도 (7.5g •L-1와 10.0g •L-1)의 blue 염색의 경우 잎의 착 색이 심하고 불균일 하였으며 꽃잎의 가장자리가 짙어 지는 현상이 나타났다.

Chrysanthemum white rust, caused by Puccinia horiana, is one of the most destructive fungal diseases in chrysanthemum cultivation worldwide. For increasing efficiency of resistant breeding, molecular markers linked to chrysanthemum white rust resistance gene were developed in pseudo F1 cross population between ‘Puma White’ as susceptible and ‘Dancer’ as resistant using bulked segregant analysis (BSA). Of 280 RAPD primers (Operon 10 mer), 18 primers found to be polymorphic. After screening of these primers in 20 individual lines, only OPI-13520 was selected as closely linked marker to white rust disease resistance. Based on correspondence between phenotypic resistant level and marker in 187 segregation population, the genetic distance between white rust resistance gene and OPI-13520 marker assumed to be 3.8 cM. For OPI-13520 marker conversion into sequence characterized amplified region (SCAR) marker, the amplified fragment of OPI-13520 was purified, cloned and sequenced. Based on the DNA sequence of OPI-13520, SCAR maker was generated and verified in 20 individual lines used in BSA-RAPD.The results showed SCAR marker could be used to identify white rust resistance in chrysanthemum.

For developing molecular markers linked to white rust resistance in chrysanthemum, RAPD and AFLP were carried out in ‘Puma White’ x ‘Dancer’ mapping population through Bulked Segregant Analysis (BSA) methods. 10 resistant and 10 susceptible individuals were selected and bulked. And then, these bulks were screened using 280 RAPD primers (10 mer) with two parents. As a result of BSA-RAPD, 25 Dancer/R-bulk specific bands in 21 primers and 22 Puma White/S-bulk specific bands in 18 primers were selected. These resistant or susceptible specific bands were screened in 10 resistant and 10 susceptible individuals. Except OPI-13520, all bands were confirmed as false positive. OPI-13520 band presumed as closely linked marker to white rust disease resistance was tested in whole population. Among 187 progenies, just six off-springs did not correspond with phenotypic data. Based on expected phenotypic segregation ratios in the pseudo F1 progenies, it was assumed that a duplex type of white rust resistance in ‘Dancer’ (RRrrrr) were in combination with a duplex type of OPI-13520 marker. As a result of x2-test of independence between resistance gene and OPI-13520 marker, x2 score is 76.08 and probability is 2.13x10-16. And resistance gene and OPI-13520 marker were assumed to be linked in coupling phase. The value of recombination fraction obtained by successive trials and second derivative of log likelihood was 0.03832±0.0271.