In vivo nicotine is associated with Alzheimer's, Parkinson's and lung cancer. Diagnostic assays of these diseases depend on very low analytical detection limits. In this study, a sensitive analytical method was examined using a voltammetric graphite pencil electrode (GPE) and a modified carbon nanotube paste electrode (CNE). The optimum analytical conditions for both electrodes were compared using square wave anodic stripping voltammetry (SW) and cyclic voltammetry (CV) obtaining 400 sec accumulation time and oxidation peak. Under optimum parameters, the stripping working range of GPE was 5.0-40.0μg/L, CNE: 0.1-0.8 and 5-50μg/L. Quantification limits were 5.0μg/L for GPE and 0.1μg/L for CNE, while detection limits were 0.6μg/L for GPE and 0.07μg/L for CNE. A standard deviation of 10.0μg/L was observed for 0.064 GPE and 0.095 CNE (n = 12) using 400 sec accumulation time. The results obtained can be applied to non.treated urine and ex vivo biological diagnostics.

This study was conducted in order to examine the effects of horticultural activities and flower tea drinking based on reminiscent storytelling on demented elders’ cognitive and emotional functions. For these an purposes, the program was executed 35 elders divided four groups at the elders institutionalized at B Facility through 10 sessions during the period from 10 March to 14 July in 2015. As a result first, in the test of pretreatment homogeneity among the groups, no significant difference was observed among the four groups, so they were considered homogeneous. As to difference between before and after the program, cognitive functions were improved significantly after the treatment in the experimental group with both horticultural activities and flower tea drinking (p=.039). Among the emotional functions, self‐esteem was significantly different in the experimental group with only horticultural activities (p=.040), the experimental group with only flower tea drinking (p=.005), and the experimental group with both horticultural activities and flower tea drinking (p=.024). Life satisfaction was significantly different only in the experimental group with both horticultural activities and flower tea drinking (p=.005). Ego integrity was significantly different in the experimental group with only horticultural activities (p=.011) and the experimental group with both horticultural activities and flower tea drinking (p=.005). Therefore, the experimental group with both horticultural activities and flower tea drinking were improved significant that all the items of cognitive and emotional functions.