Coccidiosis is caused by infection of Eimeria species and an significant parasitic disease in poultry. Various kinds of natural products have been studied to find alternative treatments for coccidiosis in chickens, but the effect of Houttuynia cordata on Eimeria infection has not been investigated. The aim of this study is to study the anticoccidial effect of H. cordata extract (HCE) in chickens after oral infection by Eimeria tenella. Anticoccidial effects of the HCE was evaluated in chickens after oral infection with E. tenella. This study was performed on threeday- old chicks (n = 30). These animals were divided into 3 groups; HCE 0.2% treated/infected (n = 10), HCE untreated/infected (n = 10) and non-infected control (n = 10). The effect of HCE on E. tenella infection was assessed by two parameters; fecal oocysts shedding and body weights gain. the chicks fed HCE significantly reduced fecal oocysts when compared to the E. tenella-infected group fed standard diets (p<0.05). Furthermore, the HCE-based diet improved weight loss due to E. tenella infection. Our data shows that HCE had significant antiprotozoal activity against E. tenella. These findings may have implications for the development of anticoccidial drugs.

Toxoplasmosis, caused by Toxoplasma gondii (Tg), is one of the most prevalent zoonotic diseases worldwide. Currently, safe and efficient therapeutic options for this disease are still being developed, and are urgently needed. Agrimonia pilosa (A. pilosa) is a rosaceous plant, and widely distributed in Asia. The bioactive compounds in A. pilosa Ledeb include flavonoid, triterpenoids, and isocoumarin, and have been reported to show many pharmacological effects including anti-inflammatory, antioxidant, and antibacterial activities.The aim of this study was to determine Anti-protozoal efficacy of Agrimonia pilosa ethanol extract (APE) against Toxoplasma gondii (Tg). In this study, APE was examined and screened for anti-Tg activity. Seventy percent ethanol was used for herbal extraction. For anti-Tg activity screening, in vitro study with APE using tachyzoit of Tg RH strain infected HeLa cells were performed. Also, in vivo anti- Tg study using the mouse infection model was conducted. As the results, the selectivity of APE was 3.35, which was higher than Sulfadiazine selectivity (2.08). Also, we performed in vivo animal study to evaluate the anti-Tg activity of APE with mouse model. As the results, Anti-toxoplasma activity of the APE was as high as sulfadiazine. These results demonstrate that it can be successfully cured against Tg infection with APE. It could be a promising native herb treatment for the prevention of Tg infection.

Helicobacter (H.) pylori is one of the most common pathogenic bacteria in the human gut, and is also one of the most important factors that cause digestive disorders such as chronic inflammation, gastric ulcers, and even gastric cancer. Since the use of various antibiotics to treat H. pylori infection is associated with the development of resistance in this bacterium, the aim of this study was to determine the anti-H. pylori effects of Peucedanum japonicum extracts (PJE) in the stomach tissue of C57BL/6 mice. In this experimental study, 50 C57BL/6 mice in five groups were evaluated. After induction of H. pylori infection in mice with the standard strain of H. pylori (ATCC 43504), the infected mice were treated with 3 different doses of PJE in different groups. Then, the anti-H. pylori effects of PJE were evaluated by rapid urease test (CLO), histopathological exam and blood interleukin (IL)-8 ELISA analysis. Based on CLO results, H. pylori eradication effects of PJE was observed. H. pylori infection of C57BL/6 mice showed significant increase of histopathological lesion scores. However, the treatment of PJE showed significant decrease of histopathological lesion scores. The treatment of PJE decreased gastric inflammation and mucosal lesions in the stomach of H. pylori-infected mice. Blood IL-8 levels were elevated 4 weeks after H. pylori inoculation. However, the treatment of PJE showed significant decrease of IL-8 level. According to the results of this study, PJE could be useful in the reduction of H. pylori infection in the mouse model.

The most frequent occurring and the serious marine casualties in fishing vessels are the collision in statistics from Korea Maritime Safety Tribunal (2008-2015). Collisions are is given a great deal of weight in all marine casualties, and the main cause of collisions is the negligence of watching. From this point of view, this study deals with the collision and its immediate cause, the negligence of watching which are main factors on the indirect causes. Basic analyzing data was gathered from the questionnaire made by experts of sea service part, and analyzed by using the fault tree analysis (FTA). From the result of the study, the occurrent probability of the negligence of watching in the collision due to the indirect causes occupied 64.9%, and its probability caused by the man factors was less than the other factors; i.e. the media factors and the management factors. For the reduction of the negligence of watching in the collision from this study, it needs an effort to remove not only the man factors, but also the media factors and management factors.