Soybean mosaic virus (SMV) resistance of Korean recommended soybeans was evaluated naturally and by mechanical inoculation in Suwon. Based on the differential reaction of forty-four soybean genotypes tested to nine different SMV strains, soybeans were classified into twenty-four groups. Myeongjunamulkong and Ilpumgeom-jeongkong showed a high degree of resistance to nine SMV strains, having no symptom. The other cultivars produced various reactions according to inoculation of each SMV strain: symptomless, mosaic or systemic necrosis. Only five cultivars such as Kwangankong, Eunhakong, Tawonkong, Namhaekong, Sobaegnamulkong were totally susceptible to every strain. There was variation in disease incidence. Soybeans, having the highest levels of resistance to G5H and G7H in the greenhouse, showed the lowest levels of SMV incidence in the field of Suwon. Myeong-junamulkong, Ilpumgeomjeongkong, Soyangkong, Pungsannamulkong, Sodamkong, Jangmikong, Geomjeong-kong2, Pureunkong, Sinpaldalkong2, Duyoukong, and Geumgangkong were fairly resistant to SMV. And SMV incidence of Taekwangkong, Saealkong and Baegunkong was over 45% with symptom of bud necrosis. And soybeans, highly resistant to SMV in the field and the greenhouse, were mainly derived from Jangyeobkong and Hwang-keumkong resistant to G1-G7.

Winter green manure crops including legume increase grain yield of subsequent crop and substitute N fertilizer requirement with organic-No Hairy vetch grows vigorously and can provide N-rich green manure for corn with its soil incorporation after wintering. But, grain yield of corn as succeeding crop would be reduced if its planting time is delayed until late spring. This experiment was carried out to find the proper incorporation time of hairy vetch green manure and planting time of subsequent corn in cropping system with winter hairy vetch(green manure)-summer corn. Hairy vetch was incorporated into soil at a ten-day interval between April 10 and May 10 and corn was planted at 5 days after each hairy vetch incorporation. Soil nitrate concentration on April 10 and 20 in hairy vetch plot was slightly lower than that at winter fallow. Above-ground dry matter and organic-N of hairy vetch increased linearly with delayed hairy vetch incorporation time from April 10 to May 10. Average dry matter and organic-N produced by hairy vetch were 5.7 ton/ha and 248 kgN/ha, respectively. Corn growth and yield decreased as delayed corn planting time after May in spite of increasing dry matter and N-yield of hairy vetch. Nitrogen concentration of corn grain, stalk and whole plant at harvest were the highest in May 5 planting, but total N-uptake of May 5 planting were not different from that of April 25 planting because of lower grain yield. It was concluded that the proper incorporation time of hairy vetch and corn planting time were April 20 and April 25, respectively, because grain yield was the highest and corn could use hairy vetch-N effectively to produce dry matter.

Reverse transcription and polymerase chain reaction (RT-PCR) assay was used to detect SMV strains. A pair of oligonucleotide primers were designed to include the cylindrical inclusion (CI) coding region between 4,176 to 5,560 nt. Amplification from the total RNA extracted from infected plants with SMV yielded a 1,385 bp DNA fragment. RT-PCR was shown to be 103 times more sensitive than the ELISA assay and it could detect a virus in 10-6 dilution. Restriction enzyme analysis of RT- PCR products using EcoR I showed that SMV isolates were classified into six groups according to the patterns of restriction fragments.